RESUMO
To evaluate the possible toxicity of the aqueous extract of Echinodorus grandiflorus in pregnant rats, animals were distributed in groups treated with 250, 500 and 1,000 mg/kg/day, by gavage, and a control group received saline solution. The treatment was carried out for 15 consecutive days, remaining during mating and until the 14(th) day of gestation. On the 15(th )day, pregnant animals were euthanized by exsanguination under anesthesia. A blood sample was destined to the hematological and biochemical analysis. The ovaries, liver, kidneys, spleen, and adrenal glands were removed and weighed. Liver, kidneys and spleen were processed for histopathological analysis. The number mated, cohabitated and pregnant rats were counted as well as the corpora lutea, implants, resorptions, and live and dead fetuses. Fetus body weight and placenta were measured. Treatment with 1,000 mg of extract caused anemia, leukocytosis, and an increase in AST and in cholesterol. The liver of animals treated with the two higher doses exhibited discrete inflammatory reaction, located mainly at the stroma which supports the portal space; in the kidneys of animals of T-500 and T-1000 groups there was an expressive decrease in the capsular space, and focal areas of vasodilatation and congestion, as well as a discrete hyalinization, and in the spleen of T-1000 group the red pulp presented excessive pigmentation suggestive of hemosiderin. There were no alterations in reproductive parameters, in fetus external morphology or in placenta weight. In conclusion, the extract causes maternal toxicity, though it does not alter the reproductive performance.
Assuntos
Alismataceae/toxicidade , Exposição Materna/efeitos adversos , Extratos Vegetais/toxicidade , Reprodução/efeitos dos fármacos , Alismataceae/química , Animais , Peso Corporal/efeitos dos fármacos , Relação Dose-Resposta a Droga , Feminino , Desenvolvimento Fetal/efeitos dos fármacos , Idade Gestacional , Tamanho do Órgão/efeitos dos fármacos , Placenta/efeitos dos fármacos , Placenta/patologia , Extratos Vegetais/isolamento & purificação , Gravidez , Ratos , Ratos Wistar , Testes de ToxicidadeRESUMO
Trichodactylus fluviatilis (Latreille, 1828) (Decapoda, Trichodactylidae) is a crab species that lives in mountainous rivers and streams at altitudes up to 500m. Its distribution ranges from South America from the Basins of eastern coast of Brazil to basins of the Upper Paraná River and Argentina. The main objective of this study was to register a new occurrence of T. fluviatilis at a Conservation Unit in Juiz de Fora, Minas Gerais and provide a new altitudinal record for this species in Brazil. The samples were taken between October 2004 and July 2005. We collected three live T. fluviatilis individuals two males and one female. We also analyzed the stomach contents of 16 Hydromedusa maximiliani specimens, of which in six were found Trichodactylidae crab body parts. Most species of Brazilian Trichodactylidae live at elevations until to 500m above sea level. In this study, T. fluviatilis was collected at elevation of 770m above sea level, expanding the range of altitudinal distribution of this species in Brazil. Prior to this study, there were no reports of T. fluviatilis in Juiz de Fora; therefore this study is a new record of this trichodatcylid in Minas Gerais State.
Trichodactylus fluviatilis (Latreille, 1828) (Decapoda, Trichodactylidae) é uma espécie de caranguejo que habita rios e riachos montanhosos em altitudes até aproximadamente 500 m. Sua distribuição ocorre na América do Sul, desde as bacias costeiras da faixa leste do Brasil até a bacia do alto Paraná e na Argentina. O objetivo do presente trabalho foi registrar a ocorrência de T. fluviatilis em uma Unidade de Conservação de Juiz de Fora, Minas Gerais, e fornecer um novo registro altitudinal dessa espécie no Brasil. As amostras foram obtidas de outubro de 2004 a julho de 2005. Foram coletados três indivíduos vivos, dois machos e uma fêmea da espécie. Também foram analisados 16 regurgitos estomacais do cágado Hydromedusa maximiliani, dos quais, em seis foram encontrados partes corporais deste crustáceo. Muitas espécies de caranguejos do Brasil da família Trichodactylidae vivem em regiões acima de 500m do nível do mar. No presente estudo, T. fluviatilis foi coletado a 770 m de altitude, ampliando assim a faixa de distribuição altitudinal dessa espécie no Brasil. Até o presente estudo não existiam relatos de T. fluviatilis no município de Juiz de Fora, portanto este estudo também se constitui em um novo registro desse tricodatilídeo em Minas Gerais.
