The airborne transmission of viruses causes tight transmission bottlenecks.

The transmission bottleneck describes the number of viral particles that initiate an infection in a new host. Previous studies have used genome sequence data to suggest that transmission bottlenecks for influenza and SARS-CoV-2 involve few viral particles, but the general principles of virus transmission are not fully understood. Here we show that, across a broad range of circumstances, tight transmission bottlenecks are a simple consequence of the physical process of airborne viral transmission. We use mathematical modelling to describe the physical process of the emission and inhalation of infectious particles, deriving the result that that the great majority of transmission bottlenecks involve few viral particles. While exceptions to this rule exist, the circumstances needed to create these exceptions are likely very rare. We thus provide a physical explanation for previous inferences of bottleneck size, while predicting that tight transmission bottlenecks prevail more generally in respiratory virus transmission.

Model for Quorum-Sensing Mediated Stochastic Biofilm Nucleation.

Surface-attached bacterial biofilms cause disease and industrial biofouling, as well as being widespread in the natural environment. Density-dependent quorum sensing is one of the mechanisms implicated in biofilm initiation. Here we present and analyze a model for quorum-sensing triggered biofilm initiation. In our model, individual, planktonic bacteria adhere to a surface, proliferate, and undergo a collective transition to a biofilm phenotype. This model predicts a stochastic transition between a loosely attached, finite layer of bacteria near the surface and a growing biofilm. The transition is governed by two key parameters: the collective transition density relative to the carrying capacity and the immigration rate relative to the detachment rate. Biofilm initiation is complex, but our model suggests that stochastic nucleation phenomena may be relevant.

A computational model for microbial colonization of an antifouling surface.

Biofouling of marine surfaces such as ship hulls is a major industrial problem. Antifouling (AF) paints delay the onset of biofouling by releasing biocidal chemicals. We present a computational model for microbial colonization of a biocide-releasing AF surface. Our model accounts for random arrival from the ocean of microorganisms with different biocide resistance levels, biocide-dependent proliferation or killing, and a transition to a biofilm state. Our computer simulations support a picture in which biocide-resistant microorganisms initially form a loosely attached layer that eventually transitions to a growing biofilm. Once the growing biofilm is established, immigrating microorganisms are shielded from the biocide, allowing more biocide-susceptible strains to proliferate. In our model, colonization of the AF surface is highly stochastic. The waiting time before the biofilm establishes is exponentially distributed, suggesting a Poisson process. The waiting time depends exponentially on both the concentration of biocide at the surface and the rate of arrival of resistant microorganisms from the ocean. Taken together our results suggest that biofouling of AF surfaces may be intrinsically stochastic and hence unpredictable, but immigration of more biocide-resistant species, as well as the biological transition to biofilm physiology, may be important factors controlling the time to biofilm establishment.

Documenting Patient Interaction: Emergency Medical Services Report Reader Expectations and Improvements for Providers.

INTRODUCTION: Patient care reports contain critical elements related to interventions rendered and medical decision-making. Yet, little consensus exists around reader expectations, leaving emergency medical services (EMS) providers unaware of critical content. OBJECTIVES: This 2-phase study aimed to answer the questions "What do EMS providers know about report readers?" and "What do report readers expect from reports?" through surveys and interviews. In doing so, this study gauged EMS providers' audience awareness of report readership and determined what readers expected from reports. METHODS: A prospective survey was conducted with 57 EMS providers to gauge their level of audience awareness or how often they thought of specific report reader groups when writing reports. Interviews were conducted with 14 report readers following retrospective think-aloud protocol, where participants verbalized their questions, comments, and concerns about reports while reading. RESULTS: Surveys indicate participants lacked a full, accurate sense of audience awareness. When writing reports, they thought of audiences, such as patients, who do not regularly read reports, while reporting not thinking of actual report readers-such as billing specialists-often or at all. Interview analysis indicated that report readers looked for 21 elements in high-quality, effective report narratives. CONCLUSIONS: These data formalize and reinforce what a high-quality narrative should include, with "high-quality" meaning the narrative allows readers to do their jobs without follow-up or an amendment needed to the report.

Growth-dependent drug susceptibility can prevent or enhance spatial expansion of a bacterial population.

As a population wave expands, organisms at the tip typically experience plentiful nutrients while those behind the front become nutrient-depleted. If the environment also contains a gradient of some inhibitor (e.g. a toxic drug), a tradeoff exists: the nutrient-rich tip is more exposed to the inhibitor, while the nutrient-starved region behind the front is less exposed. Here we show that this can lead to complex dynamics when the organism's response to the inhibitory substance is coupled to nutrient availability. We model a bacterial population which expands in a spatial gradient of antibiotic, under conditions where either fast-growing bacteria at the wave's tip, or slow-growing, resource-limited bacteria behind the front are more susceptible to the antibiotic. We find that growth-rate dependent susceptibility can have strong effects on the dynamics of the expanding population. If slow-growing bacteria are more susceptible, the population wave advances far into the inhibitory zone, leaving a trail of dead bacteria in its wake. In contrast, if fast-growing bacteria are more susceptible, the wave is blocked at a much lower concentration of antibiotic, but a large population of live bacteria remains behind the front. Our results may contribute to understanding the efficacy of different antimicrobials for spatially structured microbial populations such as biofilms, as well as the dynamics of ecological population expansions more generally.

Parasite control practices and public perception of parasitic diseases: A survey of dog and cat owners.

Drugs used in the control of internal and external parasites in companion animals play a crucial role in Animal and Public Health. To ensure continuing protection, these drugs should be administered regularly and in intervals, as suggested by the manufacturers. To assess parasite control practices and other related factors, including the degree of public awareness on the topic, 312 dog and cat owners were surveyed while attending the Small Animal Hospital, Faculty of Veterinary Medicine, Lisbon University. Results showed that 89.7% of the dogs were currently being treated with endoparasitic drugs. Of these, 74.3% were dewormed every four months or longer and merely 11.8% with the recommended treatment regimen (minimum quarterly). In cats, 63.6% were being treated with endoparasitic drugs and 85.7% of these were irregularly dewormed every four months or longer and merely 5.5% with the recommended treatment regimen (minimum quarterly). Combinations of praziquantel, pyrantel embonate and febantel were the most commonly used drugs in dogs, whereas macrocyclic lactones were more frequently used in cats. Regarding external parasitic control, 92.2% of the dogs were being treated, 50.5% of these at monthly intervals (all-year round or seasonally). The most common ectoparasitic drug formulation used on dogs was the spot-on imidacloprid+permethrin (89%). Only 28.4% of the dogs were uninterruptedly protected throughout the year from the main canine vector borne diseases transmitted by fleas, ticks, sandflies and mosquitoes. Merely 63.6% of the cats were being controlled with ectoparasitic drugs, most at infrequent drug intervals and imidacloprid was the most frequently used drug on cats (44.4%). Additionally, 85% of the respondents had never heard of the word "zoonosis" and 37% of them did not collect their dog's faeces in all public places. Scabies, toxoplasmosis and leishmaniasis were the most frequent parasitic diseases identified by the public in this survey. Although the majority of pet owners give antiparasitic drugs, our results show that most of them do not follow the manufacturers recommendations, deworming at irregular and consequently ineffective intervals. Therefore, it is of utmost importance for the veterinarians to educate pet owners regarding parasite cycles, methods of prevention and transmission mechanisms, as well as to follow the drug recommendations, in order, respectively, to increase their awareness and thereby improve the effectiveness of the available control measures.