[Anaphylactogenic, antigeni and avid properties of the antibotulin serum from the blood of various animal species]. / Anafilaktogennye, antigennye i avidnye svoistva protivobotulinicheskoi syvorotki iz krovi razlichnykh vidov zhivotnykh.

It was shown that purified and concentrated by the "Diaferm" method antibotulin sera from horse and cattle blood failed to differ by anaphylactogenic properties; at the same time in sensitization of the organism to protein of one animal species the use of the sera of another species provided a lesser reactogenicity of the preparation. The antigenic activity of the purified and concentrated sera from the blood or horses and cows in testing on rabbits was identical, but in response to cow alpha-globulin the animals responsed by a more intensive production of precipitins. The activity of cow and horse antibotulin serum (determined by the rate and stability of their association with the corresponding toxin) proved to be identical.

Histochemical investigation of some mitochondrial and microsomal enzymes in the kidneys of rabbits immunized with type B botulinus anatoxin.

Immunization of rabbits with botulinus anatoxin containing a number of proteins of bacterial origin causes a statistically significant increase in the activity of succinate dehydrogenase, NAD diaphorase and NADP diaphorase as early as after 24 hours. After 5-7 days, the activity of all mitochondrial enzymes drops below the control level and returns to normal by the 14th day. The activity of glucose 6-phosphatase decreases significantly already 24 hours after immunization and returns to normal by the end of the 7th day. The mechanism of excretion of foreign protein in the kidneys of immunized animals is discussed.

[Histological and histochemical changes in the kidneys of rabbits immunized with Cl. botulinum toxoid type B in combination with blood loss]. / Gistologicheskie i gistokhimicheskie izmeneniia v pochkakh krolikov pri immunizatsii anatoksinom Cl. botulinum tipa B v sochetanii s krovopoterei

Immunization of rabbits with increasing doses of Cl. botulinum toxoid, type B, led to the development in the kidneys of a focal intracapillary productive glomerulonephritis, and also of productive endo- and perivasculites. Blood letting (in the amount of 1% of body weight) aggravated the morphological picture of the affection on account of supervention of the alternative and exudative components. At the same time blood letting led to reduction of the NAD-diaphorase, succinic dehydrogenase and glucose-6-phosphatase activity in the epithelium of the proximal portions of the nephrons.