RESUMO
Potentiometric detection of copper ion over chemically interactive and electrically responsive chitin grafted polyaniline (Chit-g-PANI) electrode has been demonstrated for natural as well as artificial samples under optimized conditions using a portable sensing setup. The physical properties and chemical structure of developed electrode were evaluated by different Infrared spectrometry, X-ray diffraction, scanning electron microscope, thermal gravimetric analyzer and ASTM methods. The result indicates the formation of electrically responsive, chemically interactive, stable grafted hybrid matrix for durable and reproducible potentiometric electrode for a self-designed portable potentiometric cell. The potentiometric response of electrode toward Cu2+ ions follows the nernst relation in the range from 1 to 103 ppm with detection limit of 13.77 ppm and negligible interference for different co-existing cations and anions. Further, on the basis of interaction between cupric ion and Chit-g-PANI matrix, the sensing mechanism and ion to electron transduction has been discussed along with future prospects for on-site applications.
Assuntos
Compostos de Anilina/química , Quitina/química , Cobre/análise , Potenciometria/métodos , Eletrodos , Água Subterrânea/química , Concentração de Íons de Hidrogênio , Íons , Espectroscopia de Infravermelho com Transformada de Fourier , Termogravimetria , Águas Residuárias/química , Poluentes Químicos da Água/análise , Difração de Raios XRESUMO
[6]-Shogaol (1) was isolated from Zingiber officinale. Twelve novel compounds have been synthesized and evaluated for their Brugia malayi thymidylate kinase (BmTMK) inhibition activity, which plays important role for the DNA synthesis in parasite. [6]-Shogaol (1) and shogaol with thymine head group (2), 5-bromouracil head group (3), adenine head group (4) and 2-amino-3-methylpyridine head group (5) showed potential inhibitory effect on BmTMK activity. Further molecular docking studies were carried out to explore the putative binding mode of compounds 1-5.
Assuntos
Antinematódeos/síntese química , Brugia Malayi/enzimologia , Catecóis/síntese química , Desenho de Fármacos , Inibidores Enzimáticos/síntese química , Núcleosídeo-Fosfato Quinase/antagonistas & inibidores , Animais , Antinematódeos/química , Antinematódeos/isolamento & purificação , Antinematódeos/farmacologia , Brugia Malayi/efeitos dos fármacos , Catecóis/química , Catecóis/isolamento & purificação , Catecóis/farmacologia , Inibidores Enzimáticos/química , Inibidores Enzimáticos/isolamento & purificação , Inibidores Enzimáticos/farmacologia , Zingiber officinale/química , Simulação de Acoplamento Molecular , Estrutura Molecular , Núcleosídeo-Fosfato Quinase/genética , Proteínas RecombinantesRESUMO
In continuation of our drug discovery programme on Indian medicinal plants, we isolated an unusual amino acid, i.e. 2-amino-5-hydroxyhexanoic acid (1) from the seeds of Crotalaria juncea. The 2-amino-5-hydroxyhexanoic acid (1) showed dose dependent lipid lowering activity in the in vivo experiments and also showed good in vitro antioxidant activity. The cyclized compound, 3-amino-6-methyltetrahydro-2H-pyran-2-one (2) showed better lipid lowering and antioxidant profile than the parent compound 1.
