1.
Chem Commun (Camb)
; 55(67): 9979-9982, 2019 Aug 15.
Artigo
em Inglês
| MEDLINE
| ID: mdl-31367719
RESUMO
A chemical tag enhances peptide detection by multiple orders in mass spectrometry. The substantial improvement in the peptide mapping along with simplified and enhanced fragmentation pattern enables the unambiguous sequencing of a protein and antibody. The chemoselective sensitivity booster provides a tool for remarkably improved analysis of protein bioconjugates.
Assuntos
Peptídeos/análise , Proteínas/análise , Citocromos c/análise , Lisina/química , Mapeamento de Peptídeos , Sensibilidade e Especificidade , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Espectrometria de Massas em Tandem
2.
Chem Commun (Camb)
; 51(3): 473-6, 2015 Jan 11.
Artigo
em Inglês
| MEDLINE
| ID: mdl-25429646
RESUMO
This work outlines the first phthalimidation protocol suitable for protein labeling and performed in aqueous media at room temperature and neutral pH with no catalyst or co-reagent required. The methodology is suitable for a range of amines and its efficiency was determined with chemoselective and site-selective protein labeling.