RESUMO
This study is an attempt for comprehensive, combining experimental data with advanced analytical techniques and machine learning for a thorough understanding of the factors influencing the wear and cutting performance of multi-blade diamond disc cutters on granite blocks. A series of sawing experiments were performed to evaluate the wear and cutting performance of multi blade diamond disc cutters with varying diameters in the processing of large-sized granite blocks. The multi-layer diamond segments comprising the Iron (Fe) based metal matrix were brazed on the sawing blades. The segment's wear was studied through micrographs and data obtained from the Field Emission Scanning Electron Microscopy (FESEM) and Energy Dispersive X-ray (EDS). Granite rock samples of nine varieties were tested in the laboratory to determine the quantitative rock parameters. The contribution of individual rock parameters and their combined effects on wear and cutting performance of multi blade saw were correlated using statistical machine learning methods. Moreover, predictive models were developed to estimate the wear and cutting rate based on the most significant rock properties. The point load strength index, uniaxial compressive strength, and deformability, Cerchar abrasivity index, and Cerchar hardness index were found to be the significant variables affecting the sawing performance.
RESUMO
BACKGROUND: Coccinia grandis (ivy gourd), is a dioecious member of Cucurbitaceae having heteromorphic sex chromosomes. Chromosome constitution of male and female plants of C. grandis is 22A + XY and 22A + XX respectively. Earlier we showed that a unique gynomonoecious form of C. grandis (22A + XX) also exists in nature bearing morphologically hermaphrodite flowers (GyM-H). Additionally, application of silver nitrate (AgNO3) on female plants induces stamen development leading to the formation of morphologically hermaphrodite flowers (Ag-H) despite the absence of Y-chromosome. Due to the unavailability of genome sequence and the slow pace at which sex-linked genes are identified, sex expression and modification in C. grandis are not well understood. RESULTS: We have carried out a comprehensive RNA-Seq study from early-staged male, female, GyM-H, and Ag-H as well as middle-staged male and GyM-H flower buds. A de novo transcriptome was assembled using Trinity and annotated by BLAST2GO and Trinotate pipelines. The assembled transcriptome consisted of 467,233 'Trinity Transcripts' clustering into 378,860 'Trinity Genes'. Female_Early_vs_Male_Early, Ag_Early_vs_Female_Early, and GyM-H_Middle_vs_Male_Middle comparisons exhibited 35,694, 3574, and 14,954 differentially expressed transcripts respectively. Further, qRT-PCR analysis of selected candidate genes validated digital gene expression profiling results. Interestingly, ethylene response-related genes were found to be upregulated in female buds compared to male buds. Also, we observed that AgNO3 treatment suppressed ethylene responses in Ag-H flowers by downregulation of ethylene-responsive transcription factors leading to stamen development. Further, GO terms related to stamen development were enriched in early-staged male, GyM-H, and Ag-H buds compared to female buds supporting the fact that stamen growth gets arrested in female flowers. CONCLUSIONS: Suppression of ethylene responses in both male and Ag-H compared to female buds suggests a probable role of ethylene in stamen suppression similar to monoecious cucurbits such as melon and cucumber. Also, pollen fertility associated GO terms were depleted in middle-staged GyM-H buds compared to male buds indicating the necessity of Y-chromosome for pollen fertility. Overall, this study would enable identification of new sex-biased genes for further investigation of stamen arrest, pollen fertility, and AgNO3-mediated sex modification.
