Effect of Antiobiotic-Induced Disbiosis and Its Correction with Probiotics on Myocardial Tolerance to Ischemia-Reperfusion Injury in SPF Rats.

Changes in morphofunctional parameters of the isolated heart subjected to global ischemia-reperfusion were studied in SPF Wistar rats with antibiotic-induced dysbiosis (AID) treated with lyophilized yeast Saccharomyces boulardii and inactivated probiotic bacteria Lactobacillus reuteri KR2017. In contrast to S. boulardii, correction of dysbiosis with L. reuteri KR2017 against the background of gastric hypersecretion and standard antimicrobial therapy led to an increase in fat content and a decrease in free and bound water in tissues and to a significant reduction in myocardial infarct size caused by ischemia/ reperfusion injury.

[Immunotropic effects of probiotic complex bactistatin used simultaneously with antibiotics].

The immunotropic effects of the probiotic complex Bactistatin (the complex contains metabolites of Bacillus subtilis) used simultaneously with oral doxycycline during different periods of time (1, 5, 10 and 20 days) were estimated. The dynamics of the changes in the nonspecific immunity factors under the effect of the above mentioned products or their combination (bactistatin + doxycycline) during the treatment of different duration was demonstrated. It was found that the oral administration of the metabolic probiotic bactistatin to experimental animals during 1-10 days increased the activity of the nonspecific resistance factors. The immunotropic effects of the product were evident at the level of the cells of the blood phagocytic system and the processes related to synthesis of such enzymes as lysozyme and myeloperoxidase and their release into the blood stream. Comparatively rapid increase of the functional activity of the factors was registered even after a single administration of the product. When the combination of the products (bactistatin + doxycycline) was administered to the animals during a fixed period of time (10 days) significant activation of the nonspecific immunity factors was observed. In perspective the findings will allow to optimize the basic regimens of antibacterial therapy and urgent (postcontact) prevention of infectious diseases.

[Bacillus subtilis metabolites as a novel promising probiotic preparations].

Characteristics of Bacillus subtilis metabolites contained in supernatants of its broth cultures are described. Metabolites contained bacterium-produced biologically active components ensuring cells growth and propagation. These components had bacteriostatic and bactericidal effect on gram-positive and gram-negative pathogenic and conditionally pathogenic microflora of gastrointestinal tract of human and animals. Enzymes produced by the bacterium (amylase, protease, cellulose-decomposing enzyme, lipase, pectinase) increased antagonistic properties of preparation and promote its probiotic effect.

[Improvement of regimens for emergency prevention and treatment of infectious diseases].

Approaches to increasing the efficacy of the regimens for emergency prevention and treatment of infectious diseases with combined use of antibiotics, immunomodulators and probiotic metabolites are examined. On the basis of multi-factorial statistical analysis optimization of the use of such agents is discussed.

[Correction of human microbiocenosis disturbances with the help of probiotics].

The article is a review of data, revealing scientifically proven approaches to correction of human microbiocenosis disturbances with the help of microbe probiotics, produced on the basis of synergic components and properties of B. subtilis culture 3.