Societal perceptions of dentofacial appearances of patients with malocclusion: a systematic review / Percepção da sociedade sobre a aparência dento-facial de pacientes com má oclusão: revisão sistemática

Aim: This systematic review aimed to evaluate whether or not there is evidence enough to support the hypothesis that society promotes judgments on the facial aesthetics of individuals with malocclusion. Methods: Searches were conducted in the PubMed, Bireme, BBO, LILACS, Web of Science, EMBASE, Cochrane Library, and SciELO databases, supplemented by an additional manual search. Results: The present study included all articles that appeared in each of these databases between January 1965 and February 2015. Inclusion criteria were based on the articles whose primary focus was the societal perception of dentofacial appearances, written in English; observational and experimental epidemiological studies (Cross-sectional, Longitudinal, Cohort, Randomized Clinical Trial, Case-Control); and systematic reviews. Review articles, clinical case reports, laboratorial experiment studies, and abstracts were excluded. This search identified 2,530 articles, of which four fulfilled the inclusion criteria. Of these, only one study showed a high level of scientific evidence. The main flaws found included blind assessment of the measurement, validity of the measurement methods, error analysis of the method, and confounding factors not reported in all articles. Conclusion: According to this systematic review, it could be concluded that there is a need for further studies with more efficient methodological qualities.(AU)

Objetivo: Avaliar se existem evidências que suportam a hipótese de que a sociedade promove julgamentos considerando a estética facial de indivíduos com má oclusão. Material e Métodos: Foram realizadas buscas nas bases de dados PubMed, Bireme, BBO, LILACS, Web of Science, EMBASE, Cochrane Library e Scielo, complementando por uma busca manual. Resultados: Foram incluídos todos os artigos que apareceram em cada uma destas bases de dados de janeiro de 1965 a fevereiro de 2015. Critérios de inclusão foram os artigos cujo foco principal era a percepção da sociedade em relação à aparência dento-facial, publicados em inglês, estudos epidemiológicos observacionais e experimentais (Transversal, Longitudinal, Coorte, Ensaio Clínico Randomizado e Caso-Controle) e revisão sistemática. Artigos de revisão, relatos de caso clínico, estudos laboratoriais e resumos foram excluídos. A busca bibliográfica identificou 2530 artigos e 4 preencheram os critérios de inclusão. Destes somente um estudo apresentou elevado grau de evidência científica. A avaliação cega da medição, a validade dos métodos de medição, a análise de erro de método e os fatores de confusão não declarados em todos os artigos, foram as principais falhas encontradas. Conclusão: De acordo com esta revisão sistemática, concluiu-se que há a necessidade de estudos com qualidades metodológicas mais eficientes.(AU)

Modulation of expression and activity of cytochrome P450s and alteration of praziquantel kinetics during murine schistosomiasis.

In this study, we investigated the expression and activity of liver cytochrome P450s (CYPs) and praziquantel (PZQ) kinetics in mice infected with Schistosoma mansoni. Swiss Webster (SW) mice of both genders were infected (100 cercariae) on postnatal day 10 and killed on post-infection days (PIDs) 30 or 55. Non-infected mice of the same age and sex served as controls. Regardless of mouse sex, infection depressed the activities of CYP1A [ethoxy/methoxy-resorufin-O-dealkylases (EROD/MROD)], 2B9/10 [pentoxy/benzyloxy-resorufin-O-dealkylases (PROD, BROD)], 2E1 [p-nitrophenol-hydroxylase (PNPH)] and 3A11 [erythromycin N-demethylase (END)] on PID 55 but not on PID 30. On PID 55, infection decreased liver CYP mRNA levels (real-time reverse transcription-polymerase chain reaction). On PID 30, whereas mRNA levels remained unaltered in males, they were depressed in females. Plasma PZQ (200 and 400 mg/kg body weight intraperitoneally) levels were measured (high-performance liquid chromatography) at different post-treatment intervals. In males and females, infection delayed the PZQ clearance on PID 55, but not on PID 30. Therefore, it can be concluded that schistosomiasis down-modulated CYP expression and activity and delayed PZQ clearance on PID 55, when a great number of parasite eggs were lodged in the liver. On PID 30, when egg-laying was initiated by the worms, no change of CYP expression and activity was found, except for a depression of CYP1A2 and 3A11 mRNAs in female mice.

Modulation of expression and activity of cytochrome P450s and alteration of praziquantel kinetics during murine schistosomiasis

In this study, we investigated the expression and activity of liver cytochrome P450s (CYPs) and praziquantel (PZQ) kinetics in mice infected with Schistosoma mansoni. Swiss Webster (SW) mice of both genders were infected (100 cercariae) on postnatal day 10 and killed on post-infection days (PIDs) 30 or 55. Non-infected mice of the same age and sex served as controls. Regardless of mouse sex, infection depressed the activities of CYP1A [ethoxy/methoxy-resorufin-O-dealkylases (EROD/MROD)], 2B9/10 [pentoxy/benzyloxy-resorufin-O-dealkylases (PROD, BROD)], 2E1 [p-nitrophenol-hydroxylase (PNPH)] and 3A11 [erythromycin N-demethylase (END)] on PID 55 but not on PID 30. On PID 55, infection decreased liver CYP mRNA levels (real-time reverse transcription-polymerase chain reaction). On PID 30, whereas mRNA levels remained unaltered in males, they were depressed in females. Plasma PZQ (200 and 400 mg/kg body weight intraperitoneally) levels were measured (high-performance liquid chromatography) at different post-treatment intervals. In males and females, infection delayed the PZQ clearance on PID 55, but not on PID 30. Therefore, it can be concluded that schistosomiasis down-modulated CYP expression and activity and delayed PZQ clearance on PID 55, when a great number of parasite eggs were lodged in the liver. On PID 30, when egg-laying was initiated by the worms, no change of CYP expression and activity was found, except for a depression of CYP1A2 and 3A11 mRNAs in female mice.

Up- and down-modulation of liver cytochrome P450 activities and associated events in two murine malaria models.

BACKGROUND: The mechanisms by which malaria up and down-regulates CYP activities are not understood yet. It is also unclear whether CYP activities are modulated during non-lethal malaria infections. This study was undertaken to evaluate the time course of CYP alterations in lethal (Plasmodium berghei ANKA) and non-lethal (Plasmodium chabaudi chabaudi) murine malaria. Additionally, hypotheses on the association of CYP depression with enhanced nitric oxide (NO) production, and of CYP2a5 induction with endoplasmic reticulum dysfunction, enhanced haem metabolism and oxidative stress were examined as well. METHODS: Female DBA-2 and C57BL/6 mice were infected with P.berghei ANKA or P. chabaudi and killed at different post-infection days. Infection was monitored by parasitaemia rates and clinical signs. NO levels were measured in the serum. Activities of CYP1a (ethoxyresorufin-O-deethylase), 2b (benzyloxyresorufin-O-debenzylase), 2a5 (coumarin-7-hydroxylase) and uridine-diphosphoglucuronyl-transferase (UGT) were determined in liver microsomes. Glutathione-S-transferase (GST) activity and concentrations of gluthatione (GSH) and thiobarbituric acid-reactive substances (TBARS) were determined in the liver. Levels of glucose-regulated protein 78 (GRP78) were evaluated by immunoblotting, while mRNAs of haemoxygenase-1 (HO-1) and inducible nitric oxide synthase (iNOS) were determined by quantitative RT-PCR. RESULTS: Plasmodium berghei depressed CYP1a and 2b and induced 2a5 in DBA-2 mice. In P.berghei-infected C57BL/6 mice CYP activities remained unaltered. In both strains, GST and UGT were not affected by P.berghei. Plasmodium c. chabaudi depressed CYP1a and 2b and induced 2a5 activities on the day of peak parasitaemia or near this day. CYP2a5 induction was associated with over-expression of HO-1 and enhanced oxidative stress, but it was not associated with GRP78 induction, a marker of endoplasmic reticulum stress. Plasmodium chabaudi increased serum NO on days near the parasitaemia peak in both strains. Although not elevating serum NO, P.berghei enhanced iNOS mRNA expression in the liver. CONCLUSION: Down-regulation of CYP1a and 2b and induction of 2a5 occurred in lethal and non-lethal infections when parasitaemia rates were high. A contribution of NO for depression of CYP2b cannot be ruled out. Results were consistent with the view that CYP2a5 and HO-1 are concurrently up-regulated and suggested that CYP2a5 induction may occur in the absence of enhanced endoplasmic reticulum stress.