A genomic Neolithic time transect of hunter-farmer admixture in central Poland.

Ancient DNA genome-wide analyses of Neolithic individuals from central and southern Europe indicate an overall population turnover pattern in which migrating farmers from Anatolia and the Near East largely replaced autochthonous Mesolithic hunter-gatherers. However, the genetic history of the Neolithic transition in areas lying north of the European Neolithic core region involved different levels of admixture with hunter-gatherers. Here we analyse genome-wide data of 17 individuals spanning from the Middle Neolithic to the Early Bronze Age (4300-1900 BCE) in order to assess the Neolithic transition in north-central Poland, and the local impacts of hunter-farmer contacts and Late Neolithic steppe migrations. We evaluate the influence of these on local populations and assess if and how they change through time, reporting evidence of recurrent hunter-farmer admixture over three millennia, and the co-existence of unadmixed hunter-gatherers as late as 4300 BCE. During the Late Neolithic we report the appearance of steppe ancestry, but on a lesser scale than previously described for other central European regions, with evidence of stronger affinities to hunter-gatherers than to steppe pastoralists. These results help understand the Neolithic palaeogenomics of another central European area, Kuyavia, and highlight the complexity of population interactions during those times.

The genetics of an early Neolithic pastoralist from the Zagros, Iran.

The agricultural transition profoundly changed human societies. We sequenced and analysed the first genome (1.39x) of an early Neolithic woman from Ganj Dareh, in the Zagros Mountains of Iran, a site with early evidence for an economy based on goat herding, ca. 10,000 BP. We show that Western Iran was inhabited by a population genetically most similar to hunter-gatherers from the Caucasus, but distinct from the Neolithic Anatolian people who later brought food production into Europe. The inhabitants of Ganj Dareh made little direct genetic contribution to modern European populations, suggesting those of the Central Zagros were somewhat isolated from other populations of the Fertile Crescent. Runs of homozygosity are of a similar length to those from Neolithic farmers, and shorter than those of Caucasus and Western Hunter-Gatherers, suggesting that the inhabitants of Ganj Dareh did not undergo the large population bottleneck suffered by their northern neighbours. While some degree of cultural diffusion between Anatolia, Western Iran and other neighbouring regions is possible, the genetic dissimilarity between early Anatolian farmers and the inhabitants of Ganj Dareh supports a model in which Neolithic societies in these areas were distinct.

Ancient Ethiopian genome reveals extensive Eurasian admixture throughout the African continent.

Characterizing genetic diversity in Africa is a crucial step for most analyses reconstructing the evolutionary history of anatomically modern humans. However, historic migrations from Eurasia into Africa have affected many contemporary populations, confounding inferences. Here, we present a 12.5× coverage ancient genome of an Ethiopian male ("Mota") who lived approximately 4500 years ago. We use this genome to demonstrate that the Eurasian backflow into Africa came from a population closely related to Early Neolithic farmers, who had colonized Europe 4000 years earlier. The extent of this backflow was much greater than previously reported, reaching all the way to Central, West, and Southern Africa, affecting even populations such as Yoruba and Mbuti, previously thought to be relatively unadmixed, who harbor 6 to 7% Eurasian ancestry.

[Posterior capsule opacification in long-term follow-up of patients after implantation of hydrophilic/hydrophobic intraocular lens Acri.Smart]. / Opacifikácia zadného puzdra pri dlhodobom sledovaní pacientov po implantácii hydrofilnej/hydrofóbnej sosovky Acri.Smart.

AIM: The aim of the retrospective study was to establish the frequency of the posterior lens capsule opacification after the cataract surgery and artificial intraocular lens (IOL) Acri.Smart 46S (Acri.Tech Zeiss) implantation. MATERIAL AND METHODS: The study group consisted of 72 eyes (30 men and 28 women), their average age was 65.4 +/- 8.4 years, with performed cataract surgery and implanted IOL Acri.Smart 46S (Acri.Tech Zeiss). After the average period of 32.4 +/- 7.3 months after the surgery, the patients underwent the examination with refractive error measurement, uncorrected and best-corrected visual acuity for for, and posterior lens capsule transparency examination with dilated pupils and with retroillumination photography taken by NIDEK EAS-100 device. RESULTS: After the average follow-up period of 32.4 +/- 7.3 months after the surgery, the refractive error was -0.32 +/- 0.8 diopters and average cylindrical error -0.8 +/- 0.6 Dcyl. The uncorrected visual acuity (UCVA) was 0.72 +/- 0.3, and the best-corrected visual acuity (BCVA) 0.95 +/- 0.2. The average diameter of the examined posterior capsule was 5.89 +/- 2.1 mm, and the transparency of the posterior capsule was 98.41 +/- 8.5%. In two eyes (2.7%) only, the YAG capsulotomy was performed. CONCLUSION: The IOL Acri.Smart is safe, easy to implant IOL, which meets the requirements of the microincision surgery. The IOL had very low incidence of posterior lens capsule opacification, and in two eyes (2.7%) only the YAG capsulotomy was performed.

[Correlation of long-term clinical and radiological results after meniscectomies]. / Korelace dlouhodobých klinických a radiologických výsledku po meniskektomiích.

PURPOSE OF THE STUDY: Although surgical treatment of meniscus injuries has made great progress, meniscectomy remains the most frequently indicated intervention. The aim of this retrospective study was to evaluate the long-term radiological results of isolated subtotal and total meniscectomies and compare them with the clinical results. MATERIAL: The assessed group included 22 men and 8 women at an average age of 35 years; nine and 21 knees, respectively, were treated by isolated subtotal and total meniscectomy in the period from 1987 to 1989. The lateral meniscus was operated on in six knees and the medial meniscus in 24 knees. Patients with other injuries to the knee joint or with a systemic disease were not evaluated. The average follow-up was 16 years. The results were compared with the other, non-treated knee. METHODS: The results were assessed by means of the Lysholm scoring system which takes into account limping, support requirement, walking distances, instability, edema, stair ascent and descent, ability to squat and pain. Radiographic osteoarthritis of the knee was classified by the Kellgren and Lawrence system. A grade greater or equal to 2 was taken for a clear sign of arthritis. In addition, the anatomical axis of the lower extremity was evaluated. Anteroposterior projection of both knees under load was made in a standing position on long films and lateral projection was obtained separately on short films. RESULTS: The average Lysholm scores were 87 points (range, 41-100) and 91 points (range, 67-100) in the treated and non-treated knees, respectively. The outcome of meniscectomy was excellent in 14 (47 %), good in eight (27 %), satisfactory in five (16 %) and poor in three (10 %) knees. In 12 patients (40 %) the state of the treated knee was evaluated as being equal to that of the non-treated knee and in eight patients (27 %) it was even better. Osteoarthritis of the affected compartment was found in eight treated knees and that of the corresponding compartment on the opposite side in four knees. Grade 2 Kellgren- Lawrence radiographic changes were present in four, grade 3 in two and grade 4 in two knees treated by meniscectomy. The anatomic femoro-tibial angle differed between the treated and non-treated side, by 4 degrees on average (range, 2 degrees to 10 degrees ), in 13 (43 %) patients. DISCUSSION: Johnson et al. found at follow-up for about 17 years that osteoarthritis developed more often in the treated than in healthy contralateral knees (40 % as against 6 %). Tapper and Hoover reported that 45 % of men and 10 % of women were without subjective complaints at 10 to 30 years of follow up. In the relevant Czech literature, only Pasa et al. have recently paid attention to the development of lesions in knees treated by meniscectomy, but they have largely evaluated changes occurring after partial arthroscopic meniscectomies. CONCLUSIONS: The present study showed that osteoarthritis after subtotal or total meniscectomy developed in 27 % of the patients at an average follow-up of 16 years. In less than half of the patients, a deviation of the anatomical axis occurred in the treated lower extremity, as compared with the other healthy side. Lysholm scores worse in the treated than the non-treated knee were found in one third of the patients.However, a deviated knee joint axis and/or osteoarthritic lesions did not always correlate with deteriorated clinical findings.