Meta-analysis of functional expression and mutational analysis of c-Met in various cancers.

Comprehensive genomic profiling is expected to revolutionize cancer therapy. c-Met signaling is responsible for tumorigenesis in various cancers. In this prospective, we present the prevalence of c-Met mutations and copy number alterations across various solid tumors. We used major databases like cBioportal, PubMed, and COSMIC for c-Met mutation and amplification data collection from various cancers. Our result shows complete details about c-Met mutation and its clinical data of various cancers. Hotspot mutation of human c-Met protein reveals that repeatedly and most mutated regions and these hotspots may be a diagnostic tool for cancer confirmation. Amino acid and nucleotide changes and their prevalence were reported in a number of individual cancers. However, we collectively present the amino acid and nucleotide changes in various cancers in this review. Our collection of data for c-Met mutation and its distribution in different cancer tissue is showing that the missense mutation is the major one in all type of cancers. Copy number variation data showing amplification and deletion of human c-Met from various tumor types, lung and central nervous system tumors showing high amplification comparatively other types.

Nanoparticles from Actinobacteria: A Potential Target to Antimicrobial Therapy.

Nanoparticles have gained significant importance in the past two decades, due to their multifaceted applications in the field of nanomedicine. As our ecosystems and habitats are changing due to global warming, many new diseases are emerging continuously. Treating these costs a lot of money and mostly ends up in failure. In addition, frequent use of antibiotics to control the emerging diseases has led the pathogens to develop resistance to antibiotics. Hence, the nanoparticles are targeted to treat such diseases instead of the costly antibiotics. In particular, the biosynthesized nanoparticles have received considerable attention due to their simple, eco-friendly and promising activity. To highlight, microbial mediated nanoparticles have been found to possess higher activity and thus have a promising role in antimicrobial therapy to fight against the emerging drug-resistant pathogens. In this context, this review article is aimed at highlight the role of nanoparticles in the field of nanomedicine and importance of actinobacteria in the nanoparticle synthesis and their need in antimicrobial therapy. This is a comprehensive review, focusing on the potential of actinobacteria-mediated nanoparticles in the field of nanomedicine.

Bioremediation of wastewater through a quorum sensing triggered MFC: A sustainable measure for waste to energy concept.

A mission for fast advancement has constrained us to unpredictably tap various natural assets. The reckless utilisation of fossil fuels led unmanageable wastes which have greatly affected our health and environment. Endeavours to address these difficulties have conveyed to the frontal area certain creative natural solutions particularly the utilisation of microbial digestion systems. In the previous two decades, the microbial fuel cell (MFC) innovation has caught the consideration of the researchers. The MFCs is a kind of bio-electrochemical framework with novel highlights, for example, power production, wastewater treatment, and biosensor applications. Lately, dynamic patterns in MFC inquire about on its synthetic, electrochemical, and microbiological perspectives have brought about its observable applications. The MFCs have begun as a logical interest, and in numerous regards, these remaining parts to be the situation. This is especially a result of the multidimensional uses of this eco-accommodating innovation. The innovation relies upon the electroactive microorganisms, prominently known as exoelectrogens. In the first place, it is the main innovation that can create energy out of waste, without the contribution of outer/extra energy. Modification of electrodes with nanomaterials, for example, gold nanoparticles and iron oxide nanoparticles or pretreatment techniques, for example, sonication and autoclave disinfection have indicated promising outcomes in improving MFC execution for power generation and wastewater treatment. The MFC innovation has been likewise explored for the remediation of different heavy metals and hazardous components, and to recognize the poisonous components in wastewater. What's more, the MFCs can be adjusted into microbial electrolysis cells to produce hydrogen energy from different natural sources. This article gives a thorough and cutting-edge appraisal of the novel magnitudes of the MFC.

Chemical structure and biological properties of a polysaccharide isolated from Pleurotus sajor-caju.

Herein, a polysaccharide obtained from Pleurotus sajor-caju was fractionated via anion-exchange column chromatography and purified using gel permeation column chromatography. The chemical characterization of the polysaccharide indicated that it contained 90.16% total carbohydrate, 0% protein, 12.7% ash and 5.2% moisture; on the other hand, the carbon, hydrogen and nitrogen contents were found to be 31.53, 4.28 and 3.01%, respectively. The polysaccharide has the molecular weight of 79 kDa; the chemical structure of the polysaccharide is →6)α-d-Glciv(1→6)α-d-Glciii(1→6)ß-d-Glcii(1→6)α-d-Glci(1→units. The polysaccharide exhibited the DPPH radical scavenging activity of 21.67-68.35% at 10-160 µg ml-1, ABTS radical scavenging activity of 16.01-70.09% at 25-125 µg ml-1, superoxide radical scavenging activity of 24.31-73.64% at 50-250 µg ml-1, hydroxyl radical scavenging activity of 16.64-63.51% at 25-125 µg ml-1 and reducing power of 0.366-1.678% at 10-120 µg ml; further evaluation of the polysaccharide revealed its anticancer activity of 18.61-63.21% at 100-500 µg ml-1 concentration against the AGS human gastric carcinoma cell line. The active principle of the polysaccharide may be used in the food and pharmacological industry in the future.

Characterization, antimicrobial and antioxidant property of exopolysaccharide mediated silver nanoparticles synthesized by Streptomyces violaceus MM72.

Green synthesis of silver nanoparticle (SNPs) has gained considerable attention in nano-biotechnology and nano-medicine owing their potency and eco-friendliness. In this, we account the exopolysaccharide and its mediated synthesis of SNPs and their biological activities. Exopolysaccharide of the Streptomyces violaceus composed of total carbohydrate (61.4%), ash (16.1%), moisture content (1.8%) and NMR confirmed their structural composition. SNPs synthesized by the exopolysaccharide, confirmed using UV-vis spectral analysis and characterized by TEM and XRD analyses. Further, the SNPs evaluated for its antibacterial activity against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis using disc diffusion method. The SNPs has shown promising antibacterial activity were evaluated for MIC. Furthermore, the SNPs were tested for antioxidant activities and was found to have promising antioxidant activity over the standards. The above results prove that SNPs can be considered as a potent antibacterial and antioxidant drug in future.