The effectiveness of divalent cation addition for highly saline activated sludge cultures: Influence of monovalent/divalent ratio and specific cations.

Saline wastewaters are prevalent in various industries and pose challenges to stable biological treatment. Increasing monovalent cation concentrations are commonly reported to deteriorate treatment and settling performance, while divalent cations can enhance flocculation and settling. However, many previous studies were performed at relatively low salinities and reports conflict on whether concentrations of monovalent cations, divalent cations, or their ratio (M/D) are most critical. This study investigates whether addition of divalent cations shows the same benefits at high salinity (∼40 g NaCl.L-1) and whether divalent ion concentration or M/D is a better predictor of enhancement. Nine sequencing batch reactors were operated at 0.8 M NaCl or KCl monovalent salt concentration, and the concentration of divalent cations (Ca2+ and Mg2+) was varied. M/D was found to be the critical factor that consistently influenced sludge characteristics. It was particularly important in describing hydrophobicity, sludge volume index (SVI) and specific oxygen uptake rate (SOUR), with rpartial of -0.879, 0.971 and 0.966 respectively in models that had an r2adj greater than 0.93. Lower M/D also increased biomass concentrations and reduced extracellular polysaccharides, the latter which in turn correlated strongly with many shape and surface charge measures. The specific monovalent salt (Na+ or K+) influenced treatment performance, biomass concentrations, hydrophobicity, SOUR, extracellular protein and SVI. The specific divalent cation was only important in describing SVI, where Mg2+ was beneficial. Overall, this study shows that addition of divalent cations can greatly benefit high salinity activated sludge systems by improving the sludge structure, settling and organic removal.

Shock effects of monovalent cationic salts on seawater cultivated granular sludge.

Saline wastewater is commonly encountered in various industries, posing challenges to biological treatments. The application of seawater as a seed source provides a media of diverse halophilic organisms for rapid startup. However, effects of transitioning from a mixed salt source to monovalent salt solutions prevalent in industry remains unexplored. Hence, seed sludge was cultivated using seawater and later granulated under a mixed-salt synthetic medium comprising a mixture of NaCl, KCl and Na2SO4 at a combined concentration of 0.8 M (0.27 M each). The stable, acclimated granules were then tested against single salt media of 0.8 M NaCl, KCl, or Na2SO4. Shift to single salt media resulted in granule disaggregation, poor settling, sludge washout and development of fluffy or slimy flocs. Changes in exopolysaccharides composition after the single salt shift was the predominant reason for the large changes in sludge morphology. The impacts of KCl and Na2SO4 were more significant than the shift to NaCl. The resulting impacts also had a major influence on the treatment performance. A complex mechanism involving monovalent cation stimulation of proteins; ionic strength impacts on exopolysaccharides and morphology; solution density influence on sludge density and settling; and tonicity impacts on cell viability and treatment is described.