The genome sequence of a hoverfly, Merodon equestris (Fabricius, 1794).

We present a genome assembly from an individual female Merodon equestris (hoverfly; Arthropoda; Insecta; Diptera; Syrphidae). The genome sequence is 873.0 megabases in span. Most of the assembly is scaffolded into 6 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 15.95 kilobases in length.

The genome sequence of a hoverfly, Syrphus vitripennis (Meigen, 1822).

We present a genome assembly from an individual male Syrphus vitripennis (a hoverfly; Arthropoda; Insecta; Diptera; Syrphidae). The genome sequence is 388.8 megabases in span. Most of the assembly is scaffolded into 5 chromosomal pseudomolecules, including the XY sex chromosome. The mitochondrial genome has also been assembled and is 18.33 kilobases in length.

The genome sequence of a hoverfly Eristalinus aeneus (Scopoli, 1763).

We present a genome assembly from an individual female Eristalinus aeneus (a hoverfly; Arthropoda; Insecta; Diptera; Syrphidae). The genome sequence is 495.4 megabases in span. Most of the assembly is scaffolded into 6 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 15.97 kilobases in length.

The genome sequence of an ichneumonid wasp, Amblyteles armatorius (Forster, 1771).

We present a genome assembly from an individual male Amblyteles armatorius (an ichneumonid wasp; Arthropoda; Insecta; Hymenoptera; Ichneumonidae). The genome sequence is 216 megabases in span. Most of the assembly is scaffolded into 12 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 16.6 kilobases in length.

The genome sequence of the Early Bumblebee, Bombus pratorum (Linnaeus, 1761).

We present a genome assembly from an individual female Bombus pratorum (the Early Bumblebee; Arthropoda; Insecta; Hymenoptera; Apidae). The genome sequence is 285.1 megabases in span. Most of the assembly is scaffolded into 18 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 21.5 kilobases in length. Gene annotation of this assembly on Ensembl identified 13,746 protein coding genes.

The genome sequence of a snail-killing fly, Pherbina coryleti (Scopoli, 1763).

We present a genome assembly from an individual male Pherbina coryleti (snail-killing fly; Arthropoda; Insecta; Diptera; Sciomyzidae). The genome sequence is 863 megabases in span. Most of the assembly is scaffolded into six chromosomal pseudomolecules, including the assembled X sex chromosome. The mitochondrial genome has also been assembled and is 20.9 kilobases in length. Gene annotation of this assembly on Ensembl identified 32,619 protein coding genes.

The genome sequence of the Figwort Cheilosia, Cheilosia variabilis (Panzer, 1798).

We present a genome assembly from an individual male Cheilosia variabilis (the Figwort Cheilosia; Arthropoda; Insecta; Diptera; Syrphidae). The genome sequence is 414.7 megabases in span. Most of the assembly is scaffolded into 7 chromosomal pseudomolecules, including the X and Y sex chromosomes. The mitochondrial genome has also been assembled and is 16.77 kilobases in length.

The genome sequence of the Vagrant Hoverfly, Eupeodes corollae (Fabricius, 1794).

We present a genome assembly from an individual female Eupeodes corollae (the Vagrant Hoverfly; Arthropoda; Insecta; Diptera; Syrphidae). The genome sequence is 648.2 megabases in span. Most of the assembly is scaffolded into four chromosomal pseudomolecules, including with the X sex chromosome. The mitochondrial genome has also been assembled and is 18.3 kilobases in length.

The genome sequence of a bird blowfly, Protocalliphora azurea (Fallén,1817).

We present a genome assembly from an individual male Protocalliphora azurea (a bird blowfly; Arthropoda; Insecta; Diptera; Calliphoridae). The genome sequence is 874 megabases in span. Most of the assembly is scaffolded into seven chromosomal pseudomolecules, including the assembled X and Y sex chromosomes. The mitochondrial genome has also been assembled and is 15.7 kilobases in length. Gene annotation of this assembly on Ensembl identified 11,936 protein coding genes.

The genome sequence of the Common Carder Bee, Bombus pascuorum (Scopoli, 1763).

We present a genome assembly from an individual female Bombus pascuorum (the Common Carder Bee; Arthropoda; Insecta; Hymenoptera; Apidae). The genome sequence is 307.5 megabases in span. Most of the assembly is scaffolded into 17 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 21.9 kilobases in length. Gene annotation of this assembly on Ensembl identified 12,999 protein coding genes.

A sampling strategy for genome sequencing the British terrestrial arthropod fauna.

The Darwin Tree of Life (DToL) project aims to sequence and assemble high-quality genomes from all eukaryote species in Britain and Ireland, with the first phase of the project concentrating on family-level coverage plus species of particular ecological, biomedical or evolutionary interest. We summarise the processes involved in (1) assessing the UK arthropod fauna and the status of individual species on UK lists; (2) prioritising and collecting species for initial genome sequencing; (3) handling methods to ensure that high-quality genomic DNA is preserved; and (4) compiling standard operating procedures for processing specimens for genome sequencing, identification verification and voucher specimen curation. We briefly explore some lessons learned from the pilot phase of DToL and the impact of the Covid-19 pandemic.

The genome sequence of a Tiger Cranefly, Nephrotoma flavescens (Linnaeus, 1758).

We present a genome assembly from an individual male Nephrotoma flavescens (a Tiger Cranefly; Arthropoda; Insecta; Diptera; Tipulidae). The genome sequence is 1,051.3 megabases in span. Most of the assembly is scaffolded into four chromosomal pseudomolecules, including a partial X sex chromosome. The mitochondrial genome has also been assembled and is 18.9 kilobases in length. Gene annotation of this assembly on Ensembl identified 11,276 protein coding genes.

The genome sequence of a soldierfly, Nemotelus nigrinus (Fallén, 1817).

We present a genome assembly from an individual female Nemotelus nigrinus (a soldierfly; Arthropoda; Insecta; Diptera; Stratiomyidae). The genome sequence is 722.2 megabases in span. Most of the assembly is scaffolded into 5 chromosomal pseudomolecules, including the X sex chromosome. The mitochondrial genome has also been assembled and is 18.94 kilobases in length.

The genome sequence of the Buff-tailed Bumblebee, Bombus terrestris (Linnaeus, 1758).

We present a genome assembly from an individual female Bombus terrestris (the Buff-tailed Bumblebee; Arthropoda; Insecta; Hymenoptera; Apidae). The genome sequence is 393.0 megabases in span. Most of the assembly is scaffolded into 18 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 24.7 kilobases in length. Gene annotation of this assembly on Ensembl identified 14,435 protein coding genes.

The genome sequence of a bluebottle, Calliphora vomitoria (Linnaeus, 1758).

We present a genome assembly from an individual male Calliphora vomitoria (a bluebottle; Arthropoda; Insecta; Diptera; Calliphoridae). The genome sequence is 708 megabases in span. Most of the assembly is scaffolded into six chromosomal pseudomolecules, including the assembled X sex chromosome. The mitochondrial genome has also been assembled and is 16.2 kilobases in length. Gene annotation of this assembly on Ensembl identified 12,917 protein coding genes.

The genome sequence of a leaf beetle, Cryptocephalus moraei (Linnaeus, 1758).

We present a genome assembly from an individual female Cryptocephalus moraei (a leaf beetle; Arthropoda; Insecta; Coleoptera; Chrysomelidae). The genome sequence is 500.5 megabases in span. Most of the assembly is scaffolded into 15 chromosomal pseudomolecules, including the X sex chromosome. The mitochondrial genome has also been assembled and is 15.68 kilobases in length.

The genome sequence of the Locust Fly, Stomorhina lunata (Fabricius, 1805).

We present a genome assembly from an individual female Stomorhina lunata (the Locust Fly; Arthropoda; Insecta; Diptera; Rhiniidae). The genome sequence is 728.1 megabases in span. Most of the assembly is scaffolded into 6 chromosomal pseudomolecules, including the X sex chromosome. The mitochondrial genome has also been assembled and is 16.49 kilobases in length. Gene annotation of this assembly on Ensembl identified 18,358 protein coding genes.

The genome sequence of a carabid beetle, Nebria salina (Fairmaire & Laboulbène, 1854).

We present a genome assembly from an individual female Nebria salina (a carabid beetle; Arthropoda; Insecta; Coleoptera; Carabidae). The genome sequence is 256.7 megabases in span. Most of the assembly is scaffolded into 21 chromosomal pseudomolecules, including the assembled X sex chromosome. The mitochondrial genome has also been assembled and is 24.7 kilobases in length. Gene annotation of this assembly on Ensembl identified 10,671 protein coding genes.

The genome sequence of a soldier beetle, Cantharis rufa (Linnaeus, 1758).

We present a genome assembly from an individual male Cantharis rufa (soldier beetle; Arthropoda; Insecta; Coleoptera; Cantharidae). The genome sequence is 355.3 megabases in span. Most of the assembly is scaffolded into 7 chromosomal pseudomolecules, including the X sex chromosome. The mitochondrial genome has also been assembled and is 17.34 kilobases in length.

The genome sequence of a darkling beetle, Lagria hirta (Linnaeus, 1758).

We present a genome assembly from an individual male Lagria hirta (darkling beetle; Arthropoda; Insecta; Coleoptera; Tenebrionidae). The genome sequence is 336.8 megabases in span. Most of the assembly is scaffolded into 12 chromosomal pseudomolecules, including the X and Y sex chromosomes. The mitochondrial genome has also been assembled and is 16.12 kilobases in length. Gene annotation of this assembly on Ensembl identified 12,850protein coding genes.