A sampling strategy for genome sequencing the British terrestrial arthropod fauna.

The Darwin Tree of Life (DToL) project aims to sequence and assemble high-quality genomes from all eukaryote species in Britain and Ireland, with the first phase of the project concentrating on family-level coverage plus species of particular ecological, biomedical or evolutionary interest. We summarise the processes involved in (1) assessing the UK arthropod fauna and the status of individual species on UK lists; (2) prioritising and collecting species for initial genome sequencing; (3) handling methods to ensure that high-quality genomic DNA is preserved; and (4) compiling standard operating procedures for processing specimens for genome sequencing, identification verification and voucher specimen curation. We briefly explore some lessons learned from the pilot phase of DToL and the impact of the Covid-19 pandemic.

The genome sequence of the small elephant hawk moth, Deilephila porcellus (Linnaeus, 1758).

We present a genome assembly from an individual male Deilephila porcellus (the small elephant hawk moth; Arthropoda; Insecta; Lepidoptera; Sphingidae). The genome sequence is 402 megabases in span. The majority of the assembly (99.99%) is scaffolded into 29 chromosomal pseudomolecules, with the Z sex chromosome assembled.