RESUMO
The study of water soluble proteins of Trichinella spiralis has discovered for the first time some protein fractions having neiraminedase, haemagglutinative and cytotoxic activities. The presence of hyaluronidase was confirmed and its activity both for water soluble and cuticle proteins of the trichinella was examined. The haemagglutinin and cytotoxin of the trichinella have an antigen specificity. The presence of active molecules is considered as the factors of pathogenicity. These pathogenic factors were discovered in the invading larvae and adult Trichinella, while they were not found in young larvae.
Assuntos
Trichinella spiralis/patogenicidade , Animais , Hemaglutininas/metabolismo , Larva/crescimento & desenvolvimento , Larva/imunologia , Larva/metabolismo , Larva/patogenicidade , Neuraminidase/metabolismo , Proteínas/metabolismo , Ratos , Solubilidade , Trichinella spiralis/crescimento & desenvolvimento , Trichinella spiralis/imunologia , Trichinella spiralis/metabolismo , Triquinelose/etiologia , Triquinelose/parasitologiaRESUMO
Reproductive activity of mature Trichinella spiralis was studied during its cultivation in three nutrient media. Most active hatching of Trichinella larvae was observed in Erl's saline, less active in Eagle's medium and in the lactalbumin hydrolyzate medium. In Erl's medium the hatching of larvae accounted for 80% of their total mass for the first four hours of the experiment and the absolute number of larvae was 2.5 to 10 times greater than that in the two other media. The use of Erl's medium makes it possible to obtain a considerable number of Trichinella larvae in 4 to 6 hours of cultivation.