[SUSTENTOCYTE NUMBERS IN THE NEONATAL PERIOD IN THE OFFSPRING OF FEMALE RATS WITH EXPERIMENTAL LIVER DAMAGE].

On serial histological sections of the testes, stained with hematoxylin-eosin, using a morphometric device, the total numbers of spermatogenic cells and sustentocytes (Sertoli cells) were measured in the convoluted seminiferous tubules of neonatal rat pups. Experimental groups consisted of rats born from females with experimental liver damage of various origins--autoimmune (n = 33), toxic (n = 32), alcoholic (n = 12), and medicinal (n = 27). The control group included pups born from normal female rats (n = 14). In experimental rats both increase and decrease of the total number of sustentocytes was detected. In the animals of most of the experimental groups, sustentocyte cell index reflecting the ratio of the number of spermatogenic cells and sustentocytes, was decreased.

[Characteristics of Leydig cells in the newborn posterity of female rats with chronic injury of the hepatobiliary system of various genesis].

Morphological and functional features of interstitial endocrine cells (Leydig cells) in the posterity of female rats with experimental liver injury of various genesis in the neonatal period were analized. Found that in experimental rats are a reduction in the number of Leydig cells, the ratio between active and inactive endocrinocytes and as a consequence, reduction of its cell activity index.

Characteristic of sperm motility in mature offspring of females with experimental liver diseases of various origins.

We studied the effect of experimental liver injury of various origins (toxic, autoimmune, alcoholic, and mesenchymal) in the females on motility of spermatozoa in the offspring. The number of infertile (jerking and immotile) spermatozoa increased, whereas the number of fertile spermatozoa (progressive motile and slow motile) was reduced. Changes in the ratio of fertile and infertile germ cells resulted in a significant decrease in sperm motility index.

[Characteristics of the spermatogenic epithelium in the testis of newborn rats--the offspring of female rats with chronic liver injury of various genesis].

The effect of chronic injury of hepatobiliary system of various genesis in females rats on the formation of spermatogenic epithelium in the testis of their newborn offspring was studied. The models of toxic (CCl4) and medicinal (tetracycline, paracetamol) liver injury were used. The state of the seminiferous epithelium was assessed on the serial histological sections of the testis with the use of the morphometric device. In experimental animals, a reduction of the diameter and the area of the seminiferous tubules (ST) was detected, associated with the decline of the total number of spermatogenic cells, spermatogonia of various generations, total number of spermatocytes and Sertoli cells. At the same time, in the newborn rats of all the experimental groups, the number of ST with the desquamated epithelium, and that one of the multinucleated spermatogenic cells with fragmented and pyknotic nuclei were increased.

[Morphological characteristics of spermatogenesis in the offspring of female rats with chronic alcohol intoxication].

Using general histological and morphometric methods, the peculiarities of spermatogenic epithelium were studied in the offspring of female rats with chronic alcohol intoxication, which was created before the onset of pregnancy by substitution of the drinking water by 15% solution of ethyl alcohol for the period of 3 months. Total number of animals was equal to 62 rat pups which were studied at postnatal days 15, 30 and 45, including 32 rats of the intact group (10 litters) and 30 pups of the experimental group (8 litters). It was found that in the offspring of female rats with chronic alcohol intoxication, the inhibition of the processes of spermatogenesis took place, as reflected by the reduction in the area of the convoluted seminiferous tubules (CST), decrease in the number of spermatogenic cells of the seminiferous layer, increase in the proportion of CST with desquamated epithelium and giant spermatogenic cells, as well as by the reduction of spermatogenic index, which reflects the average number of layers of spermatogenic cells in each CST.

The role of experimental maternal liver pathology in the development of physiological immaturity in the offspring.

We compared the parameters of physiological maturity in the offspring of albino Wistar rats with experimental chronic liver disease of various genesis (toxic, autoimmune, and alcoholic). It was found that chronic liver disease in female rats can be a cause of physiological immaturity of the offspring (increased stillbirth rate, reduced viability, and delayed disappearance of immaturity).