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1.
Inflamm Res ; 67(7): 609-616, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-29605872

RESUMO

The pathophysiology of psoriasis is very complex and involves an interplay between immune cells and keratinocytes. The keratinocyte production of calprotectin (S100A8/A9), induced by the inflammatory psoriatic milieu, may be involved in initiating immune cell invasion, as well as in propagating inflammation. However, the exact role of calprotectin in psoriasis remains unclear. Therapeutic approaches utilizing adalimumab, etanercept and ustekinumab are widely used in psoriatic treatment, but their anti-inflammatory mechanisms are not fully understood. The aim of this study was to investigate, by immunohistochemical analysis, the expression of the heterocomplex S100A8/A9 in lesional skin from psoriatic patients undergoing biological therapy with adalimumab, etanercept or ustekinumab. Our results showed that S100A8/A9, absent or present at very low level in skin biopsies from healthy subjects, is dramatically upregulated in each epidermal layer from psoriatic patients. Interestingly, calprotectin was mainly localized in keratinocyte nuclei from psoriatic patients, suggesting a role of S100A8/A9 in keratinocyte nuclear function. Furthermore, we have shown that the biological treatment induced a drastic reduction of S100A8/A9 expression in skin biopsies from treated patients, correlating with PASI reduction. Our results suggest that calprotectin may play a crucial role as a significant marker of inflammation in psoriasis, and that its reduction of expression may be considered a favourable prognostic marker in psoriasis.


Assuntos
Adalimumab , Anti-Inflamatórios não Esteroides , Calgranulina A/imunologia , Calgranulina B/imunologia , Fármacos Dermatológicos , Etanercepte , Psoríase/imunologia , Ustekinumab , Adalimumab/farmacologia , Adalimumab/uso terapêutico , Adulto , Anti-Inflamatórios não Esteroides/farmacologia , Anti-Inflamatórios não Esteroides/uso terapêutico , Terapia Biológica , Fármacos Dermatológicos/farmacologia , Fármacos Dermatológicos/uso terapêutico , Regulação para Baixo , Etanercepte/farmacologia , Etanercepte/uso terapêutico , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Psoríase/tratamento farmacológico , Pele/efeitos dos fármacos , Pele/imunologia , Ustekinumab/farmacologia , Ustekinumab/uso terapêutico
2.
J Microsc ; 230(Pt 1): 9-15, 2008 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-18387034

RESUMO

Gold particles labelling on ultrathin sections is extensively used for antigen localization in transmission electron microscopy. In establishing absolute or relative counts in tissue sections, it would be expedient to use stereologically based unbiased estimates for quantitative results. Nowadays, quantitative immunoelectron microscopy has achieved good and satisfactory results to test whether the gold labelling follows a non-random or a random pattern and then to draw statistical comparisons between cell subcompartments within a sample of cells or between experimental groups of cells. This brief informal review of literature focuses on the relative quantitative determinations of gold labelling of antigens as well as on the statistical distribution comparisons in transmission electron microscopy.


Assuntos
Antígenos/análise , Células/química , Imuno-Histoquímica/métodos , Microscopia Imunoeletrônica/métodos , Animais , Humanos
3.
Eur J Histochem ; 45(2): 169-75, 2001.
Artigo em Inglês | MEDLINE | ID: mdl-11512638

RESUMO

The events involved in the maturation process of acinar secretory granules of rat parotid gland were investigated ultrastructurally and cytochemically by using a battery of four lectins [Triticum vulgaris agglutinin (WGA), Ulex europaeus agglutinin I (UEA-I), Glycine max agglutinin (SBA), Arachys hypogaea agglutinin (PNA)]. In order to facilitate the study, parotid glands were chronically stimulated with isoproterenol to induce secretion. Specimens were embedded in the Lowicryl K4M resin. The trans-Golgi network (TGN) derived secretory granules, which we refer to as immature secretory granules, were found to be intermediate structures in the biogenesis process of the secretory granules in the rat parotid acinar cell. These early structures do not seem to be the immediate precursor of the mature secretory granules: in fact, a subsequent interaction process between these early immature granule forms and TGN elements seems to occur, leading, finally, to the mature granules. These findings could explain the origin of the polymorphic subpopulations of the secretory granules in the normal acinar cells of the rat parotid gland. The lectin staining patterns were characteristic of each lectin. Immature and mature secretory granules were labelled with WGA, SBA, PNA, and lightly with UEA-I. Cis and intermediate cisternae of the Golgi apparatus were labelled with WGA, and trans cisternae with WGA and SBA.


Assuntos
Agonistas Adrenérgicos beta/farmacologia , Isoproterenol/farmacologia , Glândula Parótida/efeitos dos fármacos , Lectinas de Plantas , Vesículas Secretórias/efeitos dos fármacos , Proteínas de Soja , Acetilgalactosamina/metabolismo , Acetilglucosamina/metabolismo , Animais , Fucose/metabolismo , Complexo de Golgi/efeitos dos fármacos , Complexo de Golgi/metabolismo , Complexo de Golgi/ultraestrutura , Lectinas/metabolismo , Masculino , Ácido N-Acetilneuramínico/metabolismo , Glândula Parótida/citologia , Glândula Parótida/metabolismo , Aglutinina de Amendoim/metabolismo , Ratos , Ratos Wistar , Vesículas Secretórias/metabolismo , Vesículas Secretórias/ultraestrutura , Aglutininas do Germe de Trigo/metabolismo
4.
J Submicrosc Cytol Pathol ; 31(1): 115-21, 1999 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-10363359

RESUMO

Lectin staining patterns in secretory granules of rat parotid gland acinar cell of untreated and isoproterenol-injected animals were examined by electron microscopy. We used four lectin-gold complexes: Ulex europaeus agglutinin I (UEA-I), Helix pomatia agglutinin (HPA), wheat germ agglutinin (WGA), Glycine max agglutinin (SBA). Specimens were low temperature embedded in the hydrophilic Lowicryl K4M resin. The normal acinar cells produced glycoconjugates which were positive for all of the lectins used and with a characteristic topographic distribution in relation to the morphological type of granule. The cells of isoproterenol-treated rat showed marked ultrastructural changes in the size and structure of granules; significant changes in lectin binding sites in the granules were also observed.


Assuntos
Carboidratos/análise , Grânulos Citoplasmáticos/efeitos dos fármacos , Isoproterenol/farmacologia , Oligossacarídeos/análise , Glândula Parótida/efeitos dos fármacos , Animais , Sítios de Ligação , Sequência de Carboidratos , Grânulos Citoplasmáticos/ultraestrutura , Lectinas , Masculino , Dados de Sequência Molecular , Oligossacarídeos/química , Glândula Parótida/ultraestrutura , Ratos , Ratos Wistar , Valores de Referência , Sensibilidade e Especificidade
5.
FEMS Microbiol Lett ; 64(2-3): 179-82, 1991 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-1884978

RESUMO

The dihydrofolate reductase gene from Candida albicans has been cloned and partially characterized. A genomic bank from C. albicans strain 10127/5 was constructed in Escherichia coli and screened for trimethoprim resistance. A plasmid pMF1, carrying the resistance marker was isolated and characterized by restriction mapping and Southern blotting. Cells harbouring pMF1 were as sensitive as the parental cells to a wide spectrum of antibacterial agents, except for trimethoprim; the dihydrofolate reductase activity from these cells was trimethoprim resistant.


Assuntos
Candida albicans/genética , Clonagem Molecular , Tetra-Hidrofolato Desidrogenase/genética , Resistência Microbiana a Medicamentos/genética , Plasmídeos , Trimetoprima/farmacologia
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