RESUMO
This paper describes physiochemical and biological properties of 3 immunologically active compounds extracted by acetone from porcine skin. High pressure gel chromatography confirmed their heterogeneity. RP-HPLC of compound 2 (C2 or K-activin) also characterizes it as a heterogeneous entity. It was shown to inhibit proliferation of cultured fibroblasts from human fetuses and exhibit weak activity in general anaphylactic reaction (anaphylactic shock) and skin anaphylactic reaction. A summary of data on immunologically active compounds are presented with special emphasis laid on acetone-extractable K-activin.
Assuntos
Fatores Biológicos/isolamento & purificação , Pele/química , Acetona , Anafilaxia/imunologia , Animais , Fatores Biológicos/imunologia , Fatores Biológicos/farmacologia , Proliferação de Células/efeitos dos fármacos , Fibroblastos/citologia , Fibroblastos/efeitos dos fármacos , Cobaias , Humanos , Pele/efeitos dos fármacos , Pele/imunologia , Solventes , SuínosRESUMO
The influence of myelopid (MP) on the protective activity of polycomponent vaccine VP-4 prepared from the antigens of opportunistic bacteria was studied on experimental infections of mice, caused by Klebsiella pneumoniae, Salmonella typhimurium and Staphylococcus aureus. In staphylococcal and Klebsiella infections the joint administration of vaccine VP-4 and MP produced more pronounced protective effect than each of these preparations, introduced alone. The protective action of vaccine VP-4 was specially enforced by MP in cases of local staphylococcal infection. Recommendations on the joint use of two or more immunomodulating agents are possible only on the basis of the experimental substantiation of their effect in definite infections.
Assuntos
Adjuvantes Imunológicos/administração & dosagem , Infecções Bacterianas/prevenção & controle , Vacinas Bacterianas/administração & dosagem , Oligopeptídeos/administração & dosagem , Animais , Masculino , Camundongos , Modelos Animais , Vacinas Combinadas/administração & dosagemRESUMO
The effect of B-activin secreted by bone-marrow cells in response to the expression of antigen-recognizing receptors at the peak of immune response to SRBC was studied. A negative correlation between the number of immune RFC and antibody-producing cells under the influence of B-activin was established. It was shown that the decrease in the number of immune RFC was the result of precommitted B-lymphocyte maturation into plasmatic cells.