RESUMO
We developed and tested a method for fabrication of a biosensor chip based on native human whole blood lymphocyte and a titanium tipless cantilever. The biosensor can be used for measuring intermolecular adhesion forces in the cell-cell system by using atomic force spectroscopy. The developed biosensor chip was applied for measuring adhesion force between lymphocyte and granulocyte and between lymphocyte and erythrocyte in healthy individuals and in patients with acute lymphoblastic leukemia (before treatment, during standard treatment, and during relapse). It was found that adhesion force between lymphocyte and granulocyte and between lymphocyte and erythrocyte increased almost twice during relapse, which is an important diagnostic marker of early cytological abnormalities indicating progression of the disease.
Assuntos
Técnicas Biossensoriais/instrumentação , Eritrócitos/metabolismo , Granulócitos/metabolismo , Linfócitos/metabolismo , Microscopia de Força Atômica/métodos , Leucemia-Linfoma Linfoblástico de Células Precursoras/diagnóstico , Antineoplásicos/uso terapêutico , Biomarcadores/metabolismo , Estudos de Casos e Controles , Adesão Celular , Progressão da Doença , Eritrócitos/patologia , Granulócitos/patologia , Humanos , Dispositivos Lab-On-A-Chip , Linfócitos/patologia , Microscopia de Força Atômica/instrumentação , Leucemia-Linfoma Linfoblástico de Células Precursoras/tratamento farmacológico , Leucemia-Linfoma Linfoblástico de Células Precursoras/metabolismo , Leucemia-Linfoma Linfoblástico de Células Precursoras/patologia , Recidiva , Indução de Remissão , Análise Espectral/instrumentação , Análise Espectral/métodos , TitânioRESUMO
The feasibilities of the Kelvin probe mode were shown to estimate a surface potential of lymphocytes from the patients with leukemia. Having compared the values of the surface potential obtained by the traditional method of microelectrophoresis and by the Kelvin probe mode, it was established that the use of the atomic force microscope made it possible to obtain data which were of specified diagnostic importance while studying lymphocyte functional status at different stages in leucopoiesis and indicated progression of the pathological process.
Assuntos
Leucemia/metabolismo , Linfócitos/metabolismo , Potenciais da Membrana , Estudos de Casos e Controles , Eletroforese/métodos , Humanos , Leucemia/diagnóstico , Linfócitos/fisiologia , Microscopia de Força Atômica/métodosRESUMO
The effects of nanodispersed iron forms on the morphology and function of the blood system were studied. Maghemite and lepidocrocite caused a leukocytic shift towards segmented neutrophil forms, reduction of lymphocyte rigidity, and stimulated their compactization. In addition, the counts of small hyperchromatic erythrocytes with high rigidity increased in the blood flow. The results indicated that a single dose of nanodispersed iron-containing drugs improved the blood respiratory function and its microrheology.
Assuntos
Eritrócitos/efeitos dos fármacos , Compostos Férricos/toxicidade , Nanopartículas/toxicidade , Neutrófilos/efeitos dos fármacos , Animais , Animais não Endogâmicos , Forma Celular/efeitos dos fármacos , Contagem de Eritrócitos , Eritrócitos/fisiologia , Contagem de Leucócitos , Masculino , Neutrófilos/fisiologia , RatosRESUMO
Tapping mode-atomic force microscopy and force spectroscopy were used for studies of the topography of the cell surface and elastic properties of lymphocytes from healthy donors and patients with chronic lymphoblastic leukemia. It was demonstrated that the decrease in lymphocytes stiffness in patients with chronic lymphoblastic leukemia by 51.4% (p<0.05) was accompanied by spatial modification of the cell surface, in particular, increase in the number of globular protrusions and depressions by 247 and 122%, respectively (p<0.05), in comparison with normal lymphocytes.
Assuntos
Membrana Celular/ultraestrutura , Leucemia Linfocítica Crônica de Células B/sangue , Linfócitos/citologia , Linfócitos/fisiologia , Membrana Celular/fisiologia , Extensões da Superfície Celular/ultraestrutura , Elasticidade , Humanos , Linfócitos/patologia , Microscopia de Força Atômica , NanoestruturasRESUMO
We propose and tested a method for studies of native blood cells by atomic-force microscopy in a humid chamber preserving viability, size, and shape of biological objects. The method has some advantages over scanning in a liquid cell: it allows studying non-fixed blood samples in the form of suspension of live cells and excludes mechanical and chemical influences on the cells.
