[Clinical use of artificial heart valve PLANIKS]. / Klinicheskoe primenenie iskusstvenogo klapana serdtsa PLANIKS.

The first clinical results of a new repeated-disc artificial heart valve PLANIKS application were summarized. In prosthesis the useful hydrodynamic parameters, durability, reliability, convenience and easiness while implantation conduction are combined harmoniously, the sterility is guaranteed. PLANIKS permits to correct adequately the hemodynamics disorders in the early postoperative period and may be recommended for the clinical practice use.

[The laboratory diagnosis of atherogenic disorders in servicemen]. / Laboratornaia diagnostika aterogennykh narushenii u voennosluzhashchikh.

In order to calculate the atherogenic coefficient the authors have made a comparative estimation of methods for determining alfa-cholesterin in serum applying various temperature regimes during sedimentation of beta and pre-beta-lipoproteids. It was found out that the complete sedimentation of beta and pre-beta-lipoproteids could be obtained after incubation of specimen into the ice bath for 30 min. (t 0-4 degrees C) with further centrifugation at room temperature. No differences were disclosed in comparison with the reference method based on refrigerated centrifugation at 2-4 degrees C.

[Xanthine oxidase of the cerebrospinal fluid in patients with craniocerebral trauma]. / Ksantinoksidaza spinnomozgovoi zhidkosti u bol'nykh s cherepno-mozgovoi travmoi.

The authors studied the activity of xanthine oxidase (XO) and xanthine dehydrogenase (XDG) in the cerebrospinal fluid (CSF) of patients with craniocerebral trauma (CCT). XO and XDG activity in CSF appeared in moderate and severe CCT. XO in CSF was predominantly of cerebral origin. The possible role of XO in activation of lipid peroxidation in CCT is discussed. It is suggested that determination of XO and XDG activity in CSF should be used for evaluating the severity of the trauma.

[Cyclic adenosine monophosphate in the cerebrospinal fluid of patients with brain trauma and diseases]. / Tsiklicheskii adenozinmonofosfat v spinnomozgovoi zhidkosti u bol'nykh s travmami i zabolevaniiami golovnogo mozga.

The authors studied adenosine 3,5-cyclic monophosphate (cAMP) in the cerebrospinal fluid (csf) of patients with craniocerebral trauma (CCT), ischemic stroke, and brain tumors. A statistically significant increase of cAMP in csf of patients with moderate and severe CCT was revealed. In unfavourable outcomes of severe CCT the increase of cAMP continued to the time of death. The authors suggest using this index as a diagnostic criterion of the depth of the metabolic disorders in the brain tissue, as a prognostic criterion, and for purposes of differential diagnosis. The possible mechanism of lipid peroxidation activation in CCT is discussed.

[Creatine phosphokinase in the cerebrospinal fluid of patients with acute meningitis]. / Kreatinfosfokinaza v spinnomozgovoi zhidkosti u bol'nykh ostrymi meningitami.

A study is presented of the general activity and isoenzymatic spectrum of creatine phosphokinase (CPK) in the cerebrospinal fluid (CSF) in patients with acute meningitis of different etiology. The enzymatic activity proved to be increased due to the cerebral (CPK-BB) isoform. Liberation of CPK-BB from the brain tissue and its penetration into the intercellular fluid and then into the CSF was related to the functional-structural changes in the cellular membranes of the brain in meningitis.

[Determination of sialic acids in blood serum]. / Opredelenie sialovykh kislot v syvorotke krovi.

The blood serum sialic acid concentrations are recommended to be measured according to G. Sydow (1985) with the estimation of the results from the mean molar (E) and molar (E) extinction coefficients without using N-acetylneuraminic acid as the reference value. The E value for devices emitting polychromatic light (FEK-56M, KFK, KFK-2, SPECOL 211) is 132 sm-1.M-1, that for devices emitting monochromatic light (SF-4A), E = 138 sm-1.M-1. The results obtained by this method correlate with those of Hess' method in conditional units. The method is simple, no acetic acid is necessary for it, the stained complex formed is stable.

[Creatine kinase isoenzymes in the diagnosis of acute cranio-cerebral trauma]. / Izofermenty kreatinkinazy v diagnostike ostroi cherepno-mozgovoi travmy.

The cerebrospinal fluid (CSF) creatine kinase (CK) activity and isozymic spectrum (EC 2.7.3.2) have been examined in patients with craniocerebral injuries of varying severity. The CK activity has been elevated in all the patients. Three isoforms have been detected: CK-BB, CK-MB, and CK-MM. CK-BB has been detected in all the patients in the presence of the total CK activity; this is explained by the isozyme release from the brain tissue during the injury and as a result of functional and structural impairment of the cellular membranes in intensification of lipid peroxidation. The CK-MM activity is due to blood admixture in the CSF and to impaired hematoencephalic barrier during the injury. The presence of CK-MB in the CSF of patients without cardiac symptoms probably results from a recombination of CK-BB and CK-MM isoforms and is of no diagnostic significance. Measurements of the total and isozymic CK activity in the CSF of patients with craniocerebral injuries may become a test for the laboratory diagnosis of the trauma severity and course.

[Determination of lactate dehydrogenase activity using Warburg's optic test]. / Opredelenie aktivnosti laktatdegidrogenazy s primeneniem opticheskogo testa Varburga.

A method for measuring the total lactate dehydrogenase activity has been developed on the basis of Warburg's optic test with a photoelectrocolorimeter, making use of lactate as a substrate. The results are calculated from the mean molar coefficient of light absorption. The coefficient of variations of the method is 7.7%. The results obtained with the use of a spectrophotometer and photoelectrocolorimeter have been in good correlation. The described technique is recommended as a method of choice.

[Creatine phosphokinase in the cerebrospinal fluid of patients with stroke in the acute period]. / Kreatinfosfokinaza v spinnomozgovoi zhidkosti u bo'lnykh s insu'ltamiv ostrom periode.

Creatine phosphokinase (CP) activity was increased in the cerebrospinal fluid (CSF) in stroke patients as compared to a group of control patients. CP activity tended to be higher in hemorrhagic stroke as compared to ischemic one. The brain isoenzyme CP has a major impact on its total activity (above 75%). The presence of muscular CP isoenzyme in the CSF of stroke patients was explained by increased blood-brain barrier permeability, and blood admixture to SCF.