Influence of infection with Brachyspira hyodysenteriae on clinical expression, growth performance, and digestibility in growing pigs fed diets varying in type and level of fiber.

Research on the effects of different fiber types and levels on infection with Brachyspira hyodysenteriae on growth performance and nutrients digestibility in pigs is scarce. The objective of the current study was to investigate the effects of infection with B. hyodysenteriae when feeding diets varying in soluble and insoluble dietary fiber (DF) on the expression of swine dysentery, growth performance, and digestibility of organic matter (OM) nutrients. A total of 96 growing pigs (26.9 ± 2.5 kg) were used for the experiment and divided into six blocks. The growing pigs were fed one of four diets for 12 wk: low fiber (LF), high fiber (HF), high soluble fiber (HS), and high insoluble fiber (HI). After 2 wk, half of the pigs were inoculated with B. hyodysenteriae. Half of the pigs in each group were euthanized at week 6 for the measurement of the apparent digestibility at the ileum, cecum, colon, and total tract. The remaining pigs were maintained to observe and analyze the clinical expression of fecal score and excretion of B. hyodysenteriae, growth performance, and total tract digestibility up to 12 wk. In the current study, the experimental diets did not influence the expression of infection in the pigs. The body weight and average daily gain (ADG) were in line with the results of clinical expression from week 4 to 6. However, the ADG of the infected pigs started to recover from week 6 (P < 0.05) and then recovered from week 8 to 12 (P < 0.05). The infection with B. hyodysenteriae did not impair apparent ileal digestibility (AID; P > 0.05), whereas the apparent digestibility of OM, total non-starch polysaccharide, non-cellulosic polysaccharide, and cellulose in the cecum of the infected pigs was higher than non-infected pigs (P < 0.05). The apparent colonic digestibility of ash and nitrogen was higher in non-infected pigs than in infected pigs (P < 0.05). The pigs fed the LF diet had a higher digestibility in all segments of the intestinal tract, whereas the HS diet had the lowest AID but higher or similar to the LF diet in the cecum, colon, and the total tract (P < 0.05). The pigs fed the HF and HI diets, with a high proportion of insoluble fiber, had a lower digestibility in the hindgut than the other two diets (P < 0.05). In conclusion, the infection with B. hyodysenteriae negatively influenced clinical signs of swine dysentery and growth performance but did not impair AID, and neither soluble nor insoluble DF influenced the expression of the infection.

Swine dysentery is a severe disease that can cause increased mortality and poor feed efficiency with bloody diarrhea. This disease can be treated with antibiotics, but there is a limitation of using antibiotics due to governmental policy, thereby the incidence of swine dysentery has been increased. We, therefore, try to find alternatives with diverse fiber sources and understand the mechanism of swine dysentery in growing pigs. In this study, infection of Brachyspira hyodysenteriae showed a negative influence on growth performance, but compensatory growth and recovery were observed in pigs after 6 wk of the infection. The apparent ileal digestibility was not affected by the infection, and the digestibility of non-starch polysaccharides in the cecum was rather increased than decreased probably because of interaction between B. hyodysenteriae and specific bacteria, which can stimulate fiber degradation in the cecum. However, fiber type and level did not influence the prevention and alleviation of the infection.

Obesity Development and Signs of Metabolic Abnormalities in Young Göttingen Minipigs Consuming Energy Dense Diets Varying in Carbohydrate Quality.

Consumption of fructose has been associated with a higher risk of developing obesity and metabolic syndrome (MetS). The aim of this study was to examine the long-term effects of fructose compared to starch from high-amylose maize starch (HiMaize) at ad libitum feeding in a juvenile Göttingen Minipig model with 20% of the diet provided as fructose as a high-risk diet (HR, n = 15) and 20% as HiMaize as a lower-risk control diet (LR, n = 15). The intake of metabolizable energy was on average similar (p = 0.11) among diets despite increased levels of the satiety hormone PYY measured in plasma (p = 0.0005) of the LR pigs. However, after over 20 weeks of ad libitum feeding, no difference between diets was observed in daily weight gain (p = 0.103), and a difference in BW was observed only at the end of the experiment. The ad libitum feeding promoted an obese phenotype over time in both groups with increased plasma levels of glucose (p = 0.005), fructosamine (p < 0.001), insulin (p = 0.03), and HOMA-IR (p = 0.02), whereas the clinical markers of dyslipidemia were unaffected. When compared to the LR diet, fructose did not accelerate the progression of MetS associated parameters and largely failed to change markers that indicate a stimulated de novo lipogenesis.

Dietary protein source and butyrylated high-amylose maize starch included in a high-protein diet determines the urinary metabolome of rats.

Intake of red and processed meat increases the risk of colorectal cancer (CRC), whereas dairy product consumption and the intake of dietary fibre are negatively associated with this risk. We investigated the effect of (i) low-protein diets with either whey or cooked meat (beef) as the protein source and (ii) high-protein diets with cooked meat (beef) either without or with the inclusion of 10% butyrylated high-amylose maize starch (HAMSB), on the urinary metabolome of rats. Urine samples from rats were analysed using untargeted LC-MS metabolomics. The level and source of the dietary protein affected the urinary excretion of numerous metabolites indicating that several metabolic pathways were changed. The inclusion of HAMSB in a high-protein diet caused significant alterations in the excretion of several metabolites. HAMSB reduced urinary excretion of potentially harmful metabolites resulting from a high level of meat consumption.