[Characteristic of tumors developed after transplantation of transgenic GFP-positive C57BL/6 mice bone-marrow mesenchymal stem cells to mdx mice muscle].

The purpose of the study was the morphological and histochemical characteristics of differentiation of tumors developed after transplantation of GFP-positive mesenchymal bone-marrow stem cells (MSC) of transgenic mice C57BL/6 into M. quadriceps femoris of mdx mice. The tumors occurred only after transplantation of MSCs of 43-45th passages and did not arise after transplantation of MSCs of the 15th passage. No tumors developed also after transplantation of MSCs of 43-45th passages into muscle of C57BL/6 mice. The average weight of tumors appeared in 4 mdx mice studied was 1.3 +/- 0.5 g. All four tumors were classified as mesenchymomas because they originated from mesenchymal stem cells. Most of the periphery of the tumors was classified as fibrosarcomas with mitotic index 0.9 +/- 0.1%. The central parts of tumors had areas with epithelial like morphology of cells. Such cells showed positive reactivity for alcyan blue staining at pH 2.5, which indicated chondrocyte nature of the cells. No mitosis was observed in epithelial like cells. In the tumors, there were also areas with bone trabeculae containing megacaryocytes and foci of myeloid and erythrocyte hematopoiesis. There were also areas with neuronal and glial cells, and accumulations of adipocytes. One of the tumors was classified as a round cells sarcoma. The observed types of tumor cell differentiation in vivo were in accordance with described in literature types of MSCs differentiation after induction in vitro with special inductors. The spectrum of in vivo differentiation of transgenic GFP-positive MSCs after transplantation to mdx mice was broader than the spectrum of in vivo differentiation of transfected or transformed in vitro adult MSCs after transplantation to immunodeficient mice and mdx mice.

[Assessment of the histologic type of Hodgkin's disease as a prognostic factor]. / K otsenke gistologicheskogo tipa limfogranulematoza kak prognosticheskogo faktora.

The clinico-morphological data on 99 autopsied cases of Hodgkin's disease were analysed to assess the histological pattern of the disease as a prognostic factor. A correlation between the histologic pattern of the disease and lethality was established for the first 24 months after diagnosis verification. However, transformation of histological pattern was observed in 50% of cases. It is inferred that histological pattern of Hodgkin's disease can be used as a prognostic factor in planning schedule of treatment, changes in the histologic characteristics of pathological process being taken into account.

[Hematopoietic changes during fractional large-field irradiation of dogs with a subsequent bone marrow autograft]. / Izmenenie krovetvoreniia pri fraktsionirovannom krupnopol'nom obluchenii sobak s primeneniem autotransplantatsii kostnogo mozga.

After the course of fractional large field irradiation of large volumes of bone marrow is completed (a total dose of 54 Cy), autotransplantation from the nonirradiated area of bone marrow contributes to a quicker restoration of blood cells amount injured at early stages after irradiation. The complete restoration of haemopoiesis is observed in a year, and in animals with automyelotransplantation--after a half of the year and remains at the same level for 3 years.

[Prognostic significance of the lung lesions in lymphogranulomatosis]. / Prgonosticheskoe znachenie porazhenii legkikh pri limfogranulematoze.

Clinical, roentgenological and morphological data on 301 followed-up cases of Hodgkin's disease were analysed. The lung was involved in 99 patients (32.9%). Patients showing signs of intoxication, involvement of mediastinal, axillary and supraclavicular lymph nodes and prognostically unfavorable histologic patterns of lesion formed the group at high risk for lung affection. Five-year survival rate was 84.4% in the intact lung group, and 39.5%--in patients with diseased lung. As for cases of stage II and III with intact lung, the relevant figure was 86.0%--1.6 times that in cases of stage IIE-IIIE with lung lesions developing per continuitatem.