COASTAL HABITAT CHANGE AND MARINE MEGAFAUNA BEHAVIOR: FLORIDA MANATEES ENCOUNTERING REDUCED FOOD PROVISIONS IN A PROMINENT WINTER REFUGE.

A decline in submerged aquatic vegetation (SAV) within Florida's spring-fed, thermal refuges raises questions about how these systems support winter foraging of Florida manatees (Trichechus manatus latirostris). We analyzed telemetry data for 12 manatees over seven years to assess their use of Kings Bay, a winter refuge with diminished SAV. After accounting for the effect of water temperature, we hypothesized that the number of trips out of Kings Bay would increase and the time wintering manatees spent in Kings Bay would decrease. Trips out of and into Kings Bay also were compared to assess potential influences on exiting or entering. There were no detectable differences in the number of trips out of the bay or overall time manatees spent in Kings Bay across winters. The percentage of time water temperatures were below 20°C was the single best predictor of increased time spent in Kings Bay. Trips out of Kings Bay were more likely to occur after 12:00 h and during a high but ebbing tide, compared to trips into the bay. Nine manatees tracked for longer than 75 days in winter spent 7-57% of their time in the Gulf of Mexico, and three of these manatees spent 7-65% of the winter >80 km from the mouth of Kings Bay. Results suggest the low amount of SAV in Kings Bay does not obviate its use by manatees, though there are likely tradeoffs for manatees regularly foraging elsewhere. Accounting for movements of Florida manatees through a network of habitats may improve management strategies and facilitate desirable conservation outcomes.

Consortial brown tide - picocyanobacteria blooms in Guantánamo Bay, Cuba.

A brown tide bloom of Aureoumbra lagunensis developed in Guantánamo Bay, Cuba during a period of drought in 2013 that followed heavy winds and rainfall from Hurricane Sandy in late October 2012. Based on satellite images and water turbidity measurements, the bloom appeared to initiate in January 2013. The causative species (A. lagunensis) was confirmed by microscopic observation, and pigment and genetic analyses of bloom samples collected on May 28 of that year. During that time, A. lagunensis reached concentrations of 900,000 cells ml-1 (28 ppm by biovolume) in the middle portion of the Bay. Samples could not be collected from the northern (Cuban) half of the Bay because of political considerations. Subsequent sampling of the southern half of the Bay in November 2013, April 2014, and October 2014 showed persistent lower concentrations of A. lagunensis, with dominance shifting to the cyanobacterium Synechococcus (up to 33 ppm in April), an algal group that comprised a minor bloom component on May 28. Thus, unlike the brown tide bloom in Laguna Madre, which lasted 8 years, the bloom in Guantánamo Bay was short-lived, much like recent blooms in the Indian River, Florida. Although hypersaline conditions have been linked to brown tide development in the lagoons of Texas and Florida, observed euhaline conditions in Guantánamo Bay (salinity 35-36) indicate that strong hypersalinity is not a requirement for A. lagunensis bloom formation. Microzooplankton biomass dominated by ciliates was high during the observed peak of the brown tide, and ciliate abundance was high compared to other systems not impacted by brown tide. Preferential grazing by zooplankton on non-brown tide species, as shown in A. lagunensis blooms in Texas and Florida, may have been a factor in the development of the Cuban brown tide bloom. However, subsequent selection of microzooplankton capable of utilizing A. lagunensis as a primary food source may have contributed to the short-lived duration of the brown tide bloom in Guantánamo Bay.

A fresh liver agar substrate for rearing small numbers of forensically important blow flies (Diptera: Calliphoridae).

Forensically important calliphorids can be reared on a mixture of beef liver and agar. Small pieces of meat, especially fresh or frozen beef liver, will desiccate in 2-6 h, but this simple-to-make feeding substrate remains moist for at least 12 h at 25 and 30 degrees C without desiccation, even in small (5 g) amounts. We determined the survivorship of small numbers of Chrysomya megacephala (F.) (first-instar larvae to adult eclosion) raised on 5 g of liver agar and fresh beef liver. We found that all larvae raised on 5 g of liver died due to desiccation, but survivorship on 5 g of liver agar was equivalent to that on larger (50 g) pieces of either liver agar or beef liver.

Forensically important calliphoridae (diptera) associated with pig carrion in rural north-central Florida.

A study to determine the relative abundance and seasonality of forensically important blow flies (Diptera: Calliphoridae) in rural north-central Florida was conducted using pig carcasses (Sus scrofa L.) as models for human bodies. Seven species of Calliphoridae were collected: Lucilia coeruleiviridis (=Phaenicia) (Macquart), Cochliomyia macellaria (F.), Chrysomya rufifaces (Macquart), Phormia regina (Meigen), Chrysomya megacephala (F.), and a few specimens of Calliphora livida Hall, and Calliphora vicina Robineau-Desvoidy. Species composition in aerial collections of adult flies, preserved larval collections, and samples of larvae reared to the adult stage were all highly correlated. Relative abundance of the species found was significantly different, with L. coeruleiviridis the most abundant species year-round. The relative abundance of the collected species varied significantly by day of decomposition and by season, with significant interactions between season and day, season and species, and day and species. L. coeruleiviridis, C. macellaria, C. rufifaces, and P. regina were found during the entire year, two C. vicina specimens and 11 C. livida specimens were collected from December to March, whereas C. megacephala was collected only from June through September.