[Effect of immunostimulants of steroidogenesis on the activity of various oxidative enzymes in the adrenal glands and other organs]. / Vliianie immunologicheskikh stimuliatorov steroidgeneza na aktivnost' nekotorykh okislitel'nykh fermentov v nadpochechnikakh i drugikh organakh.

Effect of IgG from rabbits, immunized with deoxyribonucleoprotein and with nuclear matrix of rat adrenal glands, on activities of cytochrome c oxidase, glucose-6-phosphate (G6PD) and lactate (LDH) dehydrogenases was studied in rat adrenal glands, liver and spleen tissues, myocardial and skeletal muscles. After administration of the antibodies and deoxynucleoprotein activation of the oxidative enzymes was tissue-dependent. Activities of cytochrome c oxidase and G6PD were distinctly increased in adrenal glands. In other tissues studied the enzymatic activity was not either increased (spleen, myocardium, skeletal muscle) or mediated through adrenal glands (liver tissue). The tissue-dependent activation of the oxidative enzymes, which accompanied the steroidogenic effect of the immunoglobulins studied, was considered as a result of interaction between the antibodies and tissue-specific antigen determinants in adrenal gland chromatin.

[Effect of nonionic detergents on the results of studies of dehydrogenase and cholinesterase activities under conditions of enzyme electrophoresis]. / Vliianie neionnykh detergentov na rezul'taty issledovaniia degidrogenaznoi i kholinésteraznoi aktivnosti v usloviiakh énzim-élektroforeza.

Nonionic detergents of polyhydroxyethylene series were shown to catalyze the reduction of trinitrotetrazolium blue in reactions involving phenazinemethosulfate and other reducing agents. An approach of dehydrogenase fractions to the micells of nonionic detergents during enzyme gel electrophoresis led to an increase in colour intensity of individual isoenzymes and to redistribution of the isoenzyme spectrum as a result of catalytic effect of the detergents.