Changes in myonuclear domain size do not precede muscle hypertrophy during prolonged resistance-type exercise training.

AIM: Muscle fibre hypertrophy is accompanied by an increase in myonuclear number, an increase in myonuclear domain size or both. It has been suggested that increases in myonuclear domain size precede myonuclear accretion and subsequent muscle fibre hypertrophy during prolonged exercise training. In this study, we assessed the changes in muscle fibre size, myonuclear and satellite cell content throughout 12 weeks of resistance-type exercise training in young men. METHODS: Twenty-two young men (23 ± 1 year) were assigned to a progressive, 12-weeks resistance-type exercise training programme (3 sessions per week). Muscle biopsies from the vastus lateralis muscle were taken before and after 2, 4, 8 and 12 weeks of exercise training. Muscle fibre size, myonuclear content, myonuclear domain size and satellite cell content were assessed by immunohistochemistry. RESULTS: Type I and type II muscle fibre size increased gradually throughout the 12 weeks of training (type I: 18 ± 5%, type II: 41 ± 6%, P < 0.01). Myonuclear content increased significantly over time in both the type I (P < 0.01) and type II (P < 0.001) muscle fibres. No changes in type I and type II myonuclear domain size were observed at any time point throughout the intervention. Satellite cell content increased significantly over time in both type I and type II muscle fibres (P < 0.001). CONCLUSION: Increases in myonuclear domain size do not appear to drive myonuclear accretion and muscle fibre hypertrophy during prolonged resistance-type exercise training in vivo in humans.

Secretory activity and postembryonic development of the tentacle sensory system controlling growth hormone-producing neurons in Lymnaea stagnalis.

The cerebral neuroendocrine peptidergic light green cells (LGC) of the freshwater snail Lymnaea stagnalis regulate body growth. The LGC are controlled by a tentacle sensory system that consists of two types (S1 and S2) of primary sensory neuron located at the base of each tentacle. Sensory (S2) axons make synaptic contacts (type A synapse-like structures) with the somata and axons of the LGC, where they release the contents of secretory granules, by exocytosis (demonstrated with the ultrastructural tannic acid-Ringer incubation method). Ultracytochemistry indicates that the granule contents are glycoproteinaceous. Furthermore, the S2 axons release secretory material in a nonsynaptic fashion into the interneuronal space of the central nervous system (CNS), at the level of the neuropiles of the cerebral ganglia and of the cerebral commissure. This release occurs by exocytosis from nonsynaptic release sites. It is proposed that the tentacle sensory system not only (synaptically) controls LGC activity but also influences other, remote neuronal targets in the CNS in a nonsynaptic ("at long distance," "paracrine," "hormone-like") fashion. Already in newly hatched snails (with a shell height of 1 mm) S2 axons show a fair rate of exocytotic activity, in both synaptic and nonsynaptic respects. During postembryonic development the secretory capacity of the S2 sensory neurons increases markedly, by increases in (1) the number of axons, (2) the size of the secretory granules, and (3) exocytosis activity. This increased capacity may meet a growing demand of the developing CNS, including the LGC, for neurochemical input from the tentacle sensory system.

Medical, social and psychological recovery after cardiac rehabilitation.

A multidisciplinary study was undertaken to determine the number of patients that recovered, deteriorated or remained unchanged during a cardiac rehabilitation programme, as assessed in medical, social and psychological terms. In addition, the relationship between the medical, social and psychological aspects of recovery were investigated. Criteria for improvement, deterioration and an unchanged condition had been developed for the different aspects of recovery. These criteria were based on the degree of change during the rehabilitation programme (expressed in an effect size index) and the outcome at completion of the programme (expressed in terms of good, moderate or poor according to external standards). Although more patients improved than deteriorated, quite a few patients remained unchanged in medical, social or psychological condition. We conclude that cardiac rehabilitation might not be necessary for some patients and is not sufficient for others. Further, the relationship between the several aspects of recovery investigated was found to be weak.