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1.
Nat Immunol ; 19(4): 318-320, 2018 04.
Artigo em Inglês | MEDLINE | ID: mdl-29563627
3.
Sci Transl Med ; 8(337): 337ra65, 2016 05 04.
Artigo em Inglês | MEDLINE | ID: mdl-27147589

RESUMO

Thymic stromal lymphopoietin (TSLP), interleukin-25 (IL-25), and IL-33 are important initiators of type 2-associated mucosal inflammation and immunity. However, their role in the maintenance of progressive type 2 inflammation and fibrosis is much less clear. Using chronic models of helminth infection and allergic lung inflammation, we show that collective disruption of TSLP, IL-25, and IL-33 signaling suppresses chronic and progressive type 2 cytokine-driven inflammation and fibrosis. In a schistosome lung granuloma model or during chronic Schistosoma mansoni infection in the liver, individual ablation of TSLP, IL-25, or IL-33/ST2 had no impact on the development of IL-4/IL-13-dependent inflammation or fibrosis. However, significant reductions in granuloma-associated eosinophils, hepatic fibrosis, and IL-13-producing type 2 innate lymphoid cells (ILC2s) were observed when signaling of all three mediators was simultaneously disrupted. Combined blockade through monoclonal antibody (mAb) treatment also reduced IL-5 and IL-13 expression during primary and secondary granuloma formation in the lungs. In a model of chronic house dust mite-induced allergic lung inflammation, combined mAb treatment did not decrease established inflammation or fibrosis. TSLP/IL-33 double-knockout mice treated with anti-IL-25 mAb during priming, however, displayed decreased inflammation, mucus production, and lung remodeling in the chronic phase. Together, these studies reveal partially redundant roles for TSLP, IL-25, and IL-33 in the maintenance of type 2 pathology and suggest that in some settings, early combined targeting of these mediators is necessary to ameliorate progressive type 2-driven disease.


Assuntos
Citocinas/metabolismo , Fibrose/imunologia , Inflamação/imunologia , Inflamação/terapia , Interleucina-17/metabolismo , Interleucina-33/metabolismo , Neoplasias Pulmonares/imunologia , Animais , Anticorpos Monoclonais/uso terapêutico , Citocinas/antagonistas & inibidores , Citocinas/genética , Feminino , Fibrose/tratamento farmacológico , Fibrose/terapia , Granuloma/tratamento farmacológico , Granuloma/imunologia , Granuloma/parasitologia , Granuloma/terapia , Inflamação/tratamento farmacológico , Interleucina-13/antagonistas & inibidores , Interleucina-13/genética , Interleucina-13/metabolismo , Interleucina-17/antagonistas & inibidores , Interleucina-17/genética , Interleucina-33/antagonistas & inibidores , Interleucina-33/genética , Interleucina-4/antagonistas & inibidores , Interleucina-4/genética , Interleucina-4/metabolismo , Neoplasias Pulmonares/tratamento farmacológico , Neoplasias Pulmonares/parasitologia , Neoplasias Pulmonares/terapia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Camundongos Knockout , Schistosoma mansoni/imunologia , Schistosoma mansoni/patogenicidade , Linfopoietina do Estroma do Timo
4.
Am J Pathol ; 186(4): 885-98, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26908008

RESUMO

Although a clear association has been established between IL-33 and inflammatory bowel disease, mechanistic studies to date, primarily using acute murine models of colitis, have yielded contradicting results, demonstrating both pathogenic and protective roles. We used a well-characterized, spontaneous model of inflammatory bowel disease [ie, SAMP1/YitFc (SAMP) mice] to investigate the role of IL-33 during chronic intestinal inflammation. Our results showed marked eosinophil infiltration into the gut mucosa with increased levels of eotaxins and type 2 helper T-cell (Th2) cytokines as disease progressed and became more severe, which could be reversed upon either eosinophil depletion or blockade of IL-33 signaling. Exogenous IL-33 administration recapitulated these effects in ilea of uninflamed (parental) control AKR/J mice. Human data supported these findings, showing colocalization and up-regulation of IL-33 and eosinophils in the colonic mucosa of inflammatory bowel disease patients versus noninflamed controls. Finally, colonization of commensal flora by fecal material transplantation into germ-free SAMP and the presence of the gut microbiome induced IL-33, subsequent eosinophil infiltration, and mounting of Th2 immune responses, leading to exacerbation of chronic intestinal inflammation characteristic of SAMP mice. These data demonstrate a pathogenic role for IL-33-mediated eosinophilia and activation of Th2 immunity in chronic intestinal inflammation that is dependent on the gut microbiome. Targeting IL-33 may represent a novel therapeutic approach to treat patients with inflammatory bowel disease.


Assuntos
Eosinófilos/citologia , Ileíte/patologia , Interleucina-33/metabolismo , Células Th2/imunologia , Animais , Citocinas/metabolismo , Modelos Animais de Doenças , Ileíte/imunologia , Inflamação/imunologia , Inflamação/metabolismo , Mucosa Intestinal/metabolismo , Camundongos , Regulação para Cima
5.
Immun Inflamm Dis ; 3(3): 239-46, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-26417439

RESUMO

Interleukin (IL)-33 is a cytokine of the IL-1 family, which signals through the ST2 receptor. Previous work demonstrated that the systemic administration of recombinant IL-33 reduces the development of atherosclerosis in apolipoprotein E-deficient (ApoE(-/-)) mice by inducing a Th1-to-Th2 shift. The objective of our study was to examine the role of endogenous IL-33 and ST2 in atherosclerosis. ApoE(-/-), IL-33(-/-)ApoE(-/-), and ST2(-/-)ApoE(-/-) mice were fed with a cholesterol-rich diet for 10 weeks. Additionally, a group of ApoE(-/-) mice was injected with a neutralizing anti-ST2 or an isotype control antibody during the period of the cholesterol-rich diet. Atherosclerotic lesion development was measured by Oil Red O staining in the thoracic-abdominal aorta and the aortic sinus. There were no significant differences in the lipid-staining area of IL-33(-/-)ApoE(-/-), ST2(-/-)ApoE(-/-), or anti-ST2 antibody-treated ApoE(-/-) mice, compared to ApoE(-/-) controls. The absence of IL-33 signaling had no major and consistent impact on the Th1/Th2 cytokine responses in the supernatant of in vitro-stimulated lymph node cells. In summary, deficiency of the endogenously produced IL-33 and its receptor ST2 does not impact the development of atherosclerosis in ApoE-deficient mice.

6.
J Allergy Clin Immunol ; 133(5): 1448-55, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-24220317

RESUMO

BACKGROUND: Exercise-induced bronchoconstriction (EIB) is a prototypical feature of indirect airway hyperresponsiveness. Mast cells are implicated in EIB, but the characteristics, regulation, and function of mast cells in patients with EIB are poorly understood. OBJECTIVES: We sought to examine mast cell infiltration of the airway epithelium in patients with EIB and the regulation of mast cell phenotype and function by epithelially derived cytokines. METHODS: Endobronchial biopsy specimens, epithelial brushings, and induced sputum were obtained from asthmatic patients with and without EIB and healthy control subjects. Mast cell proteases were quantified by using quantitative PCR, and mast cell density was quantified by using design-based stereology. Airway epithelial responses to wounding and osmotic stress were assessed in primary airway epithelial cells and ex vivo murine lung tissue. Mast cell granule development and function were examined in cord blood-derived mast cells. RESULTS: Tryptase and carboxypeptidase A3 expression in epithelial brushings and epithelial mast cell density were selectively increased in the asthma group with EIB. An in vitro scratch wound initiated the release of thymic stromal lymphopoietin, which was greater in epithelial cells derived from asthmatic patients. Osmotic stress induced the release of IL-33 from explanted murine lungs, which was increased in allergen-treated mice. Thymic stromal lymphopoietin combined with IL-33 increased tryptase and carboxypeptidase A3 immunostaining in mast cell precursors and selectively increased cysteinyl leukotriene formation by mast cells in a manner that was independent of in vitro sensitization. CONCLUSIONS: Mast cell infiltration of the epithelium is a critical determinant of indirect airway hyperresponsiveness, and the airway epithelium might serve as an important regulator of the development and function of this mast cell population.


Assuntos
Asma Induzida por Exercício/imunologia , Citocinas/imunologia , Regulação da Expressão Gênica/imunologia , Interleucinas/imunologia , Mastócitos/imunologia , Mucosa Respiratória/imunologia , Animais , Asma Induzida por Exercício/patologia , Linhagem Celular , Feminino , Humanos , Interleucina-33 , Pulmão/imunologia , Pulmão/patologia , Masculino , Mastócitos/patologia , Camundongos , Mucosa Respiratória/patologia , Escarro/imunologia , Linfopoietina do Estroma do Timo
7.
Arthritis Res Ther ; 15(1): R13, 2013 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-23324173

RESUMO

INTRODUCTION: Interleukin (IL)-33 is a cytokine of the IL-1 family, which signals through the ST2 receptor. Previous work suggested implication of the IL-33/ST2 axis in the pathogenesis of human and mouse arthritis. Here, we directly investigated the role of endogenous IL-33 in K/BxN serum transfer-induced arthritis by using IL-33 knockout (KO) mice. METHODS: Arthritis was induced by injection of complete K/BxN serum or purified IgG. Disease severity was monitored by clinical and histological scoring. RESULTS: K/BxN serum transfer induced pronounced arthritis with similar incidence and severity in IL-33 KO and wild-type (WT) mice. In contrast, disease development was significantly reduced in ST2 KO mice. IL-33 expression in synovial tissue was comparable in arthritic WT and ST2 KO mice, and absent in IL-33 KO mice. Transfer of purified arthritogenic IgG instead of complete K/BxN serum also resulted in similar arthritis severity in IL-33 KO and WT mice, excluding a contribution of IL-33 contained in the serum of donor mice to explain this result. We investigated additional potential confounding factors, including purity of genetic background, but the mechanisms underlying reduced arthritis in ST2 KO mice remained unclear. CONCLUSIONS: The data obtained with IL-33 KO mice indicate that endogenous IL-33 is not required for the development of joint inflammation in K/BxN serum transfer-induced arthritis. On the contrary, arthritis severity was reduced in ST2 KO mice. This observation might relate to IL-33 independent effects of ST2, and/or reveal the existence of confounding variables affecting the severity of joint inflammation in these KO strains.


Assuntos
Artrite Experimental/imunologia , Artrite Reumatoide/imunologia , Interleucinas/imunologia , Animais , Artrite Experimental/metabolismo , Artrite Reumatoide/metabolismo , Genótipo , Imuno-Histoquímica , Proteína 1 Semelhante a Receptor de Interleucina-1 , Interleucina-33 , Interleucinas/deficiência , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Fenótipo , Reação em Cadeia da Polimerase em Tempo Real , Receptores de Interleucina/deficiência , Receptores de Interleucina/imunologia
8.
Nanotoxicology ; 7(6): 1070-81, 2013 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-22686327

RESUMO

Allergic asthma is a chronic inflammatory disorder of the airway associated with bronchial obstruction, airway hyper-reactivity (AHR), and mucus production. The epithelium may direct and propagate asthmatic-like responses. Central to this theory is the observation that viruses, air pollution, and allergens promote epithelial damage and trigger the generation of IL-25, IL-33, and TSLP via innate pathways such as TLRs and purinergic receptors. Similarly, engineered nanomaterials promote a Th2-associated pathophysiology. In this study, we tested the hypothesis that instillation of multi-walled carbon nanotubes (MWCNT) impair pulmonary function in C57Bl/6 mice due to the development of IL-33-dependent Th2-associated inflammation. MWCNT exposure resulted in elevated levels of IL-33 in the lavage fluid (likely originating from airway epithelial cells), enhanced AHR, eosinophil recruitment, and production of Th2-associated cytokines and chemokines. Moreover, these events were dependent on IL-13 signaling and the IL-33/ST2 axis, but independent of T and B cells. Finally, MWCNT exposure resulted in the recruitment of innate lymphoid cells. Collectively, our data suggest that MWCNT induce epithelial damage that results in release of IL-33, which in turn promotes innate lymphoid cell recruitment and the development of IL-13-dependent inflammatory response.


Assuntos
Imunidade Inata/efeitos dos fármacos , Interleucinas/metabolismo , Pulmão/citologia , Pulmão/efeitos dos fármacos , Nanotubos de Carbono/toxicidade , Hipersensibilidade Respiratória/induzido quimicamente , Animais , Linhagem Celular , Células Epiteliais/efeitos dos fármacos , Proteínas de Homeodomínio/genética , Proteínas de Homeodomínio/metabolismo , Inflamação/metabolismo , Interleucina-13/genética , Interleucina-13/metabolismo , Interleucina-33 , Interleucinas/genética , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Nanotubos de Carbono/química , Alvéolos Pulmonares/citologia , Alvéolos Pulmonares/fisiologia , Linfócitos T Auxiliares-Indutores/fisiologia
9.
Proc Natl Acad Sci U S A ; 110(1): 282-7, 2013 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-23248269

RESUMO

Parasitic helminths are a major cause of chronic human disease, affecting more than 3 billion people worldwide. Host protection against most parasitic helminths relies upon Type 2 cytokine production, but the mechanisms that regulate interleukin (IL) 4 and 13 production from CD4(+) T helper 2 cells (T(H)2) and innate lymphoid type 2 cells (ILC2s) remain incompletely understood. The epithelial cell-derived cytokines IL-25 and IL-33 promote Type 2 responses, but the extent of functional redundancy between these cytokines is unclear and whether Type 2 memory relies upon either IL-25 or IL-33 is unknown. Herein, we demonstrate a pivotal role for IL-33 in driving primary and anamnestic immunity against the rodent hookworm Nippostrongylus brasiliensis. IL-33-deficient mice have a selective defect in ILC2-derived IL-13 during both primary and secondary challenge infections but generate stronger canonical CD4(+) T helper 2 cells responses (IL-4, IgE, mast cells, and basophils) than WT controls. Lack of IL-13 production in IL-33-deficient mice impairs resistin-like molecule beta (RELMß) expression and eosinophil recruitment, which are two mechanisms that eliminate N. brasiliensis parasites from infected hosts. Thus, IL-33 is requisite for IL-13 but not IL-4-driven Type 2 responses during hookworm infection.


Assuntos
Infecções por Uncinaria/imunologia , Interleucina-13/imunologia , Interleucinas/imunologia , Nippostrongylus/imunologia , Células Th2/imunologia , Análise de Variância , Animais , Eosinófilos/imunologia , Citometria de Fluxo , Hormônios Ectópicos/imunologia , Peptídeos e Proteínas de Sinalização Intercelular , Interleucina-33 , Interleucinas/deficiência , Camundongos , Reação em Cadeia da Polimerase em Tempo Real
10.
Proc Natl Acad Sci U S A ; 109(26): 10486-91, 2012 Jun 26.
Artigo em Inglês | MEDLINE | ID: mdl-22689946

RESUMO

The synthetic double-stranded RNA poly(I:C) is commonly used as an adjuvant to boost CD8 T-cell function; however, polyinosinic:polycytidylic acid [poly(I:C)] can also suppress autoimmune disease. The mechanism by which a single adjuvant achieves two distinct immunoregulatory roles is unknown. Although it is clear that coadministration of poly(I:C) with antigen elicits strong adjuvant effects in mice, we found that poly(I:C) injection before antigen substantially reduced antigen-dependent CD8 T-cell responses. Notably, CD8 T cells sensitized in poly(I:C)-pretreated mice failed to fully up-regulate IL-33R (ST2), which led to impaired T-cell receptor-independent responses to IL-33. In contrast, nonsensitized effector CD8 T cells responded robustly to IL-33 using a two-signal cytokine mechanism. During an acute lung response to Staphylococcus aureus enterotoxin, peripheral injection of poly(I:C) manifested a suppressive process by inhibiting the differentiation of both antigen- and IL-33-responsive CD8 effectors systemically. These findings highlight that early exposure to double-stranded RNA reverses its role as an adjuvant and, importantly, prevents IL-33R up-regulation on CD8 effector T cells to dampen inflammation.


Assuntos
Linfócitos T CD8-Positivos/citologia , Diferenciação Celular/fisiologia , Interleucinas/fisiologia , Receptor 3 Toll-Like/metabolismo , Animais , Antígenos/imunologia , Linfócitos T CD8-Positivos/imunologia , Interleucina-33 , Ligantes , Ativação Linfocitária , Camundongos , RNA de Cadeia Dupla/administração & dosagem
11.
J Exp Med ; 209(3): 607-22, 2012 Mar 12.
Artigo em Inglês | MEDLINE | ID: mdl-22329990

RESUMO

The molecular mechanisms that drive mucosal T helper type 2 (T(H)2) responses against parasitic helminths and allergens remain unclear. In this study, we demonstrate in mice that TFF2 (trefoil factor 2), an epithelial cell-derived repair molecule, is needed for the control of lung injury caused by the hookworm parasite Nippostrongylus brasiliensis and for type 2 immunity after infection. TFF2 is also necessary for the rapid production of IL-33, a T(H)2-promoting cytokine, by lung epithelia, alveolar macrophages, and inflammatory dendritic cells in infected mice. TFF2 also increases the severity of allergic lung disease caused by house dust mite antigens or IL-13. Moreover, TFF2 messenger RNA expression is significantly increased in nasal mucosal brushings during asthma exacerbations in children. These experiments extend the biological functions of TFF2 from tissue repair to the initiation and maintenance of mucosal T(H)2 responses.


Assuntos
Asma/imunologia , Infecções por Uncinaria/imunologia , Interleucinas/biossíntese , Mucinas/imunologia , Proteínas Musculares/imunologia , Peptídeos/imunologia , Animais , Criança , Humanos , Imunidade nas Mucosas , Interleucina-33 , Pulmão/imunologia , Macrófagos Alveolares/imunologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Mucinas/deficiência , Mucinas/genética , Proteínas Musculares/deficiência , Proteínas Musculares/genética , Nippostrongylus , Peptídeos/deficiência , Peptídeos/genética , RNA Mensageiro/genética , Células Th2/imunologia , Fator Trefoil-2
12.
J Appl Physiol (1985) ; 112(6): 1015-22, 2012 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-22174404

RESUMO

Interleukin (IL)-1ß is involved in several brain functions, including sleep regulation. It promotes non-rapid eye movement (NREM) sleep via the IL-1 type I receptor. IL-1ß/IL-1 receptor complex signaling requires adaptor proteins, e.g., the IL-1 receptor brain-specific accessory protein (AcPb). We have cloned and characterized rat AcPb, which shares substantial homologies with mouse AcPb and, compared with AcP, is preferentially expressed in the brain. Furthermore, rat somatosensory cortex AcPb mRNA varied across the day with sleep propensity, increased after sleep deprivation, and was induced by somnogenic doses of IL-1ß. Duration of NREM sleep was slightly shorter and duration of REM sleep was slightly longer in AcPb knockout than wild-type mice. In response to lipopolysaccharide, which is used to induce IL-1ß, sleep responses were exaggerated in AcPb knockout mice, suggesting that, in normal mice, inflammation-mediated sleep responses are attenuated by AcPb. We conclude that AcPb has a role in sleep responses to inflammatory stimuli and, possibly, in physiological sleep regulation.


Assuntos
Encéfalo/fisiologia , Proteína Acessória do Receptor de Interleucina-1/metabolismo , Receptores Tipo I de Interleucina-1/metabolismo , Sono REM/fisiologia , Sono/fisiologia , Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Animais , Encéfalo/efeitos dos fármacos , Encéfalo/metabolismo , Inflamação/metabolismo , Inflamação/fisiopatologia , Interleucina-1beta/metabolismo , Interleucina-1beta/farmacologia , Lipopolissacarídeos/farmacologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , RNA Mensageiro/metabolismo , Ratos , Ratos Sprague-Dawley , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/fisiologia , Sono/efeitos dos fármacos , Sono REM/efeitos dos fármacos
13.
J Allergy Clin Immunol ; 129(1): 216-27.e1-6, 2012 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-22119406

RESUMO

BACKGROUND: Asthma has been considered an immunologic disease mediated by T(H)2 cells and adaptive immunity. However, clinical and experimental observations suggest that additional pathways might regulate asthma, particularly in its nonallergic forms, such as asthma associated with air pollution, stress, obesity, and infection. OBJECTIVES: Our goal was to understand T(H)2 cell-independent conditions that might lead to airway hyperreactivity (AHR), a cardinal feature of asthma. METHODS: We examined a murine model of experimental asthma in which AHR was induced with glycolipid antigens, which activate natural killer T (NKT) cells. RESULTS: In this model AHR developed rapidly when mice were treated with NKT cell-activating glycolipid antigens, even in the absence of conventional CD4(+) T cells. The activated NKT cells directly induced alveolar macrophages to produce IL-33, which in turn activated NKT cells, as well as natural helper cells, a newly described non-T, non-B, innate lymphoid cell type, to increase production of IL-13. Surprisingly, this glycolipid-induced AHR pathway required not only IL-13 but also IL-33 and its receptor, ST2, because it was blocked by an anti-ST2 mAb and was greatly reduced in ST2(-/-) mice. When adoptively transferred into IL-13(-/-) mice, both wild-type natural helper cells and NKT cells were sufficient for the development of glycolipid-induced AHR. CONCLUSION: Because plant pollens, house dust, and some bacteria contain glycolipids that can directly activate NKT cells, these studies suggest that AHR and asthma can fully develop or be greatly enhanced through innate immune mechanisms involving IL-33, natural helper cells, and NKT cells.


Assuntos
Imunidade Adaptativa , Asma/imunologia , Imunidade Inata , Interleucinas/metabolismo , Linfócitos/imunologia , Transferência Adotiva , Animais , Asma/induzido quimicamente , Asma/metabolismo , Células Dendríticas/imunologia , Modelos Animais de Doenças , Feminino , Galactosilceramidas/administração & dosagem , Glicolipídeos/imunologia , Proteína 1 Semelhante a Receptor de Interleucina-1 , Interleucina-13/biossíntese , Interleucina-33 , Interleucinas/biossíntese , Pulmão/imunologia , Pulmão/metabolismo , Pulmão/patologia , Macrófagos/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Camundongos Knockout , Células T Matadoras Naturais/imunologia , Receptores de Interleucina/genética , Receptores de Interleucina/metabolismo , Sphingomonas/imunologia , Células Th2/imunologia
14.
J Neurosci ; 31(49): 18048-59, 2011 Dec 07.
Artigo em Inglês | MEDLINE | ID: mdl-22159118

RESUMO

In the CNS, interleukin-1ß (IL-1ß) is synthesized and released during injury, infection, and disease, mediating inflammatory responses. However, IL-1ß is also present in the brain under physiological conditions, and can influence hippocampal neuronal function. Several cell-specific IL-1-mediated signaling pathways and functions have been identified in neurons and astrocytes, but their mechanisms have not been fully defined. In astrocytes, IL-1ß induced both the p38 MAPK and NF-κB (nuclear factor κB) pathways regulating inflammatory responses, however in hippocampal neurons IL-1ß activated p38 but not NF-κB. Additionally, IL-1ß induced Src phosphorylation at 0.01 ng/ml in hippocampal neurons, a dose 1000-fold lower than that used to stimulate inflammatory responses. IL-1 signaling requires the type 1 IL-1 receptor and the IL-1 receptor accessory protein (IL-1RAcP) as a receptor partner. We previously reported a novel isoform of the IL-1RAcP, IL-1RAcPb, found exclusively in CNS neurons. In this study, we demonstrate that AcPb specifically mediates IL-1ß activation of p-Src and potentiation of NMDA-induced calcium influx in mouse hippocampal neurons in a dose-dependent manner. Mice lacking the AcPb, but retaining the AcP, isoform were deficient in IL-1ß regulation of p-Src in neurons. AcPb also played a modulatory role in the activation of p38 MAPK, but had no effect on NF-κB signaling. The restricted expression of AcPb in CNS neurons, therefore, governs specific neuronal signaling and functional responses to IL-1ß.


Assuntos
Regulação da Expressão Gênica/efeitos dos fármacos , Proteína Acessória do Receptor de Interleucina-1/metabolismo , Interleucina-1beta/farmacologia , Neurônios/efeitos dos fármacos , Isoformas de Proteínas/metabolismo , Análise de Variância , Animais , Astrócitos/efeitos dos fármacos , Astrócitos/fisiologia , Cálcio/metabolismo , Células Cultivadas , Relação Dose-Resposta a Droga , Embrião de Mamíferos , Ativação Enzimática/efeitos dos fármacos , Ativação Enzimática/genética , Feminino , Regulação da Expressão Gênica/fisiologia , Hipocampo/citologia , Imunoprecipitação , Proteína Acessória do Receptor de Interleucina-1/deficiência , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , N-Metilaspartato/farmacologia , NF-kappa B/genética , NF-kappa B/metabolismo , Fosforilação/efeitos dos fármacos , Gravidez , Isoformas de Proteínas/deficiência , Receptores de N-Metil-D-Aspartato/metabolismo , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/genética , Fatores de Tempo , Transfecção , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo
15.
Nat Immunol ; 12(7): 631-8, 2011 May 29.
Artigo em Inglês | MEDLINE | ID: mdl-21623379

RESUMO

Patients with asthma, a major public health problem, are at high risk for serious disease from influenza virus infection, but the pathogenic mechanisms by which influenza A causes airway disease and asthma are not fully known. We show here in a mouse model that influenza infection acutely induced airway hyper-reactivity (AHR), a cardinal feature of asthma, independently of T helper type 2 (T(H)2) cells and adaptive immunity. Instead, influenza infection induced AHR through a previously unknown pathway that required the interleukin 13 (IL-13)-IL-33 axis and cells of the non-T cell, non-B cell innate lymphoid type called 'natural helper cells'. Infection with influenza A virus, which activates the NLRP3 inflammasome, resulted in much more production of IL-33 by alveolar macrophages, which in turn activated natural helper cells producing substantial IL-13.


Assuntos
Imunidade Adaptativa , Hiper-Reatividade Brônquica/imunologia , Linfócitos/imunologia , Infecções por Orthomyxoviridae/imunologia , Animais , Asma/imunologia , Asma/fisiopatologia , Proteínas de Transporte/imunologia , Feminino , Imunidade Inata , Vírus da Influenza A/imunologia , Interleucina-13/imunologia , Interleucina-33 , Interleucinas/imunologia , Macrófagos Alveolares/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Proteína 3 que Contém Domínio de Pirina da Família NLR , Linfócitos T Auxiliares-Indutores/imunologia
16.
J Leukoc Biol ; 89(3): 383-92, 2011 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-20952658

RESUMO

Cytokines are key mediators of the immune system, and few have been more thoroughly studied than those of the IL-1 family. IL-1α and IL-1ß are the founding members and now celebrate 25 years since their cloning. In that time, IL-1-directed research has illuminated many aspects of cytokine biology and innate immunity. The family is now recognized to include 11 total members, including IL-18 and IL-33, which are the topic of this review. These two inflammatory cytokines are expressed broadly, and their actions influence a variety of physiologic responses involved in inflammation and immunity. The purpose of this article is not to provide an exhaustive review of IL-18 and IL-33 but rather, to summarize what is known about their key functions and to provide perspective on their similarities and differences.


Assuntos
Interleucina-18/imunologia , Interleucina-1/imunologia , Interleucinas/imunologia , Animais , Evolução Molecular , Humanos , Interleucina-33 , Ligação Proteica , Solubilidade
17.
Nat Rev Immunol ; 10(2): 89-102, 2010 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-20081871

RESUMO

Over recent years it has become increasingly clear that innate immune responses can shape the adaptive immune response. Among the most potent molecules of the innate immune system are the interleukin-1 (IL-1) family members. These evolutionarily ancient cytokines are made by and act on innate immune cells to influence their survival and function. In addition, they act directly on lymphocytes to reinforce certain adaptive immune responses. This Review provides an overview of both the long-established and more recently characterized members of the IL-1 family. In addition to their effects on immune cells, their involvement in human disease and disease models is discussed.


Assuntos
Imunidade Adaptativa , Imunidade Inata , Interleucina-1/imunologia , Animais , Doenças Autoimunes/imunologia , Doenças Cardiovasculares/imunologia , Humanos , Inflamação/imunologia , Interleucina-1/metabolismo , Linfócitos T/imunologia , Linfócitos T/metabolismo
18.
Immunity ; 30(6): 817-31, 2009 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-19481478

RESUMO

Interleukin-1 (IL-1) has multiple functions in both the periphery and the central nervous system (CNS) and is regulated at many levels. We identified an isoform of the IL-1 receptor (IL-1R) accessory protein (termed AcPb) that is expressed exclusively in the CNS. AcPb interacted with IL-1 and the IL-1R but was unable to mediate canonical IL-1 responses. AcPb expression, however, modulated neuronal gene expression in response to IL-1 treatment in vitro. Animals lacking AcPb demonstrated an intact peripheral IL-1 response and developed experimental autoimmune encephalomyelitis (EAE) similarly to wild-type mice. AcPb-deficient mice were instead more vulnerable to local inflammatory challenge in the CNS and suffered enhanced neuronal degeneration as compared to AcP-deficient or wild-type mice. These findings implicate AcPb as an additional component of the highly regulated IL-1 system and suggest that it may play a role in modulating CNS responses to IL-1 and the interplay between inflammation and neuronal survival.


Assuntos
Processamento Alternativo , Sistema Nervoso Central/imunologia , Proteína Acessória do Receptor de Interleucina-1/metabolismo , Interleucina-1/metabolismo , Neurônios/imunologia , Sequência de Aminoácidos , Animais , Astrócitos/imunologia , Sequência de Bases , Encéfalo/citologia , Encéfalo/imunologia , Linhagem Celular Tumoral , Células Cultivadas , Citocinas/imunologia , Citocinas/metabolismo , Humanos , Inflamação/imunologia , Proteína Acessória do Receptor de Interleucina-1/química , Proteína Acessória do Receptor de Interleucina-1/genética , Camundongos , Camundongos Knockout , Dados de Sequência Molecular , Isoformas de Proteínas/química , Isoformas de Proteínas/genética , Isoformas de Proteínas/metabolismo , Transdução de Sinais/imunologia
19.
Arthritis Rheum ; 60(3): 738-49, 2009 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-19248109

RESUMO

OBJECTIVE: Interleukin-33 (IL-33; or, IL-1F11) was recently identified as the ligand of the IL-1 family receptor T1/ST2. The aim of this study was to examine IL-33 production in human and mouse joints and to investigate the role of IL-33 and T1/ST2 in experimental arthritis. METHODS: IL-33 expression was examined in human synovial tissue, rheumatoid arthritis (RA) synovial fibroblasts, and arthritic mouse joints. Mice with collagen-induced arthritis (CIA) were treated with blocking anti-ST2 antibody or control antibody beginning at the onset of disease. Arthritis severity was assessed by clinical and histologic scoring. Draining lymph node (LN) cell responses were examined ex vivo, and joint messenger RNA (mRNA) was used for expression profiling. RESULTS: IL-33 was highly expressed in human RA synovium. In cultured synovial fibroblasts, IL-33 expression was strongly induced by IL-1beta and/or tumor necrosis factor alpha. Furthermore, IL-33 mRNA was detected in the joints of mice with CIA and increased during the early phase of the disease. Administration of a blocking anti-ST2 antibody at the onset of disease attenuated the severity of CIA and reduced joint destruction. Anti-ST2 antibody treatment was associated with a marked decrease in interferon-gamma production as well as with a more limited reduction in IL-17 production by ex vivo-stimulated draining LN cells. Finally, RANKL mRNA levels in the joint were reduced by anti-ST2 treatment. CONCLUSION: IL-33 is produced locally in inflamed joints, and neutralization of IL-33 signaling has a therapeutic effect on the course of arthritis. These observations suggest that locally produced IL-33 may contribute to the pathogenesis of joint inflammation and destruction.


Assuntos
Artrite Experimental/metabolismo , Artrite Experimental/patologia , Interleucinas/metabolismo , Índice de Gravidade de Doença , Transdução de Sinais/fisiologia , Membrana Sinovial/metabolismo , Membrana Sinovial/patologia , Idoso , Animais , Anticorpos Anti-Idiotípicos/farmacologia , Artrite Experimental/induzido quimicamente , Artrite Reumatoide/metabolismo , Artrite Reumatoide/patologia , Células Cultivadas , Colágeno , Modelos Animais de Doenças , Feminino , Humanos , Interferon gama/metabolismo , Proteína 1 Semelhante a Receptor de Interleucina-1 , Interleucina-17/metabolismo , Interleucina-33 , Masculino , Proteínas de Membrana/antagonistas & inibidores , Proteínas de Membrana/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos DBA , Pessoa de Meia-Idade , Ligante RANK/metabolismo , RNA Mensageiro/metabolismo , Receptores de Interleucina
20.
J Allergy Clin Immunol ; 123(2): 472-8, 2009 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-19064280

RESUMO

BACKGROUND: In steady state, hemopoietic progenitors constantly egress from the bone marrow (BM) into the blood and circulate through the peripheral tissues. In allergic diseases, the BM releases increased numbers of CD34(+) progenitor cells that migrate to the site of allergic inflammation, where they differentiate into tissue-dwelling and classic effector cells of allergy, such as mast cells, eosinophils, and basophils. OBJECTIVE: To examine whether peripheral blood CD34(+) cells in addition to being progenitors may also directly function as inflammatory effector cells. METHODS: Highly purified neonatal or adult blood CD34(+) cells were examined for the expression of thymic stromal lymphopoietin (TSLP) and IL-33 receptors and for their response to these cytokines as well as to supernatants of primary small airway epithelial cells and nasal explants from rhinosinusitis and control subjects. Sputum of patients with asthma was examined before and after allergen inhalation for the presence of IL-5 and IL-13-containing CD34(+) cells. RESULTS: Circulating CD34(+) cells expressed receptors for TSLP and IL-33 and responded to these cytokines by rapidly releasing high levels of proinflammatory T(H)2-like cytokines and chemokines. These cells were activated in a TSLP-dependent manner by the supernatant fluids from activated primary human small airway epithelial cells and from nasal explants of patients with chronic rhinosinusitis. Moreover, activated CD34(+) cells containing IL-5 and IL-13 could be detected in the sputum of individuals with allergic asthma, with numbers increasing in response to specific allergen inhalation challenge. CONCLUSION: Blood CD34(+) cells, in addition to being progenitors, may act as proinflammatory effector cells by themselves and directly contribute to the allergic inflammation.


Assuntos
Antígenos CD34/imunologia , Asma/imunologia , Células-Tronco Hematopoéticas/imunologia , Inflamação/imunologia , Alérgenos/imunologia , Alérgenos/metabolismo , Antígenos CD34/metabolismo , Asma/metabolismo , Diferenciação Celular/imunologia , Citocinas/biossíntese , Citocinas/imunologia , Células-Tronco Hematopoéticas/efeitos dos fármacos , Células-Tronco Hematopoéticas/metabolismo , Humanos , Inflamação/metabolismo , Interleucina-33 , Interleucinas/imunologia , Receptores de Citocinas/imunologia , Receptores de Citocinas/metabolismo , Escarro/imunologia , Escarro/metabolismo , Linfopoietina do Estroma do Timo
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