RESUMO
The extended-spectrum beta-lactamase, TEM-63, was identified in three separate genera of South African isolates: Proteus mirabilis, Klebsiella pneumoniae, and Escherichia coli. This paper describes identification of the gene in these isolates and compares relative rates of hyrolysis between TEM-63 and other known ceftazidimases.
Assuntos
Enterobacteriaceae/enzimologia , Enterobacteriaceae/genética , Klebsiella pneumoniae/enzimologia , Proteus mirabilis/enzimologia , beta-Lactamases/metabolismo , Substituição de Aminoácidos , Clonagem Molecular , África do Sul , beta-Lactamases/biossíntese , beta-Lactamases/química , beta-Lactamases/genéticaRESUMO
beta-lactamases of Gram-negative bacteria are evolving dynamically. New developments include the production of enzymes with novel substrate profiles, reduced susceptibility to beta-lactamase inhibitors, and the simultaneous production of multiple types of beta-lactamases. The changes represent evolutionary upgrades which provide modern pathogens with a greater potential to resist beta-lactam antibiotics and cause formidable therapeutic, infection control, and diagnostic challenges. This review is a clinically oriented outline of recent developments in the beta-lactamase production of Gram-negative bacteria.