[Design of experimental approaches on the base of standard proteins for testing blood biopreparations and immunoperoxidase conjugates specific to human and animal immunoglobulines G].

Using standard forms of immunoglobulin (Ig) G and albumin, we have studied electrophoretic and chromatographic profiles of samples of pharmaceutical blood biopreparation batches. The usability of standard proteins was also demonstrated by testing analytical characteristics of immunoperoxidase conjugates specific to human and animal IgG (anti-IgG IPC). In particular, we suggest an additional estimation of analytical characteristics of anti-IgG IPC by the enzyme reaction kinetics with the standard dilution which is calculated by the direct enzyme-liked immunoassay on the homologous IgG-antigen.

[The nucleotide sequences of the HN gene and F gene fragment of Newcastle disease virus strains BOR74 and BOR82]. / Nukleotidnye posledovatel'nosti gena HN i fragmenta gena F shtammov BOR74 i BOR82 virusa N'iukaslskoi bolezni.

The complete nucleotide sequence of HN gene, the region of F gene, and intergene regions (M-F, F-HN, and HN-L) of the BOR74 and BOR82 strains of Newcastle disease virus have been determined. Based on the nucleotide and amino acid sequences, the speeds of the nucleic and amino acid changes were calculated (approximately 10(-3) nucleotides or amino acids/year). The BOR strains were grouped phylogenetically with the asymptomatic strains. These strains and the BOR strains have the same motif of the cleavage site (112GKQGR116-L117), but the HN protein of BOR strains has the 572 amino acids which differ the BOR strains from all other strains (571, 577, and 616 amino acids).

[Study of the virus carrier state in chicken influenza]. / Izuchenie virusonositel'stva pri grippe kur.

The problems of virus carrier state in influenza are connected with two aspects of the disease: the duration of virus antigen persistence in convalescents and changes of influenza virus properties in the course of persistence. In the present study, natural influenza infection in chickens caused by influenza A/chicken/USSR/336/74 virus (Hav6H3--N2) was used to determine the duration of virus antigen persistence (up to 60 days) and the entire period of virus isolation from the survivers (up to 30 days). Administration of hydrocortisone on the 50th day of convalescence permitted to obtain from the chickens several influenza A virus isolates antigenically unrelated to the epizootic strain either in hemagglutinin or in neuraminidase. Cultivation of isolate No. 42 (Hav7Neq1) in the presence of the homologous serum yielded strain 42' which was neutralized by the serum to Hav6H3--N2 virus. The isolates differed from the epizootic virus by their biological properties: the eluting activity, pathogenesis and morphology. The above facts of antigenic variability are considered in the light of the antigenic heterogeneity of the natural virus population and the possibility of virus activation by the provoking effect of extreme conditions on the carriers of latent infection.

[Antigenic characteristics of chicken influenza viruses]. / Antigennaia kharakteristika virusov grippa kur

Data on isolation of chicken influenza virus strains in this country during epizootics in 1970--1975 are presented, and the antigenic characteristics of the isolates are described. All the strains had a mixed hemagglutinin type (GP6-H3N2) and neuraminidase H2. Neuraminidase of the strains had more marked antigenic relationship with H2 human influenza A/Singapore/57 (H2N2) virus than H2 of other human influenza virus strains. The size of KM of neuraminidase is similar in all chicken influenza virus strains their antigenic formula is suggested [A(GP6-H3N2)].