[Surface properties of Lactobacillus strains. I. Hemagglutination and hydrophobicity]. / Wlasciwosci powierzchniowe bakterii z rodzaju Lactobacillus. I. Wlasciwosci hemaglutynacyjne i hydrofobowe.

Bacteria possess many surface membrane properties, both mechanical and biochemical, that allow them to interact with their environment These properties may affect a host in either positive (beneficial) or negative (pathological) ways. All surface properties of bacteria are yet unknown therefore we attempt to increase our knowledge regarding specific strains of lactobacilli, by examining the known properties including hemagglutination and hydrophobicity. The purpose of this study was to evaluate the cell surface properties of certain strains of Lactobacillus. These strains isolated from the human vagina and gastrointestinal tract were selected because of their antagonism toward aerobic and anaerobic bacterial pathogens. Part I discusses the hydrophobicity and hemagglutination abilities of these Lactobacillus strains.

[Surface properties of Lactobacillus strains. II. Adherence to tissue culture surfaces]. / Wlasciwosci powierzchniowe bakterii z rodzaju Lactobacillus. II. Adherencja do linii komórkowych.

In the second part of this study, we evaluated the adherence properties of specific strains of Lactobacillus isolated from both the human vagina and gastrointestinal tracts. Lactobacilli taken from the vagina and GI tract were tested for their adherence to A431 vaginal tissue, and to CaCo-2 cells taken from the gastrointestinal tract. The Lactobacillus strains with the most marked adherence to the respective cell lines were examined under the electron microscope. These images revealed the presence of a substance morphologically resembling slime, which probably possess unknown active properties.

[Evaluation of bacteriological research data and laboratory symptoms of infection in the diagnosis of congenital and acquired infections]. / Ocena wyników badán bakteriologicznych oraz laboratoryjnych wykladników infekcji w rozpoznawaniu zakazen wrodzonych oraz nabytych.

The predisposition of the newborns to contract infections diseases is dependent upon the limited efficiency of their immune mechanisms. Congenital infections amount to 5.7% in the research material, and the acquired infections 1.15%. The isolation of the microorganism is the basis for treating infections-the profiles of the pathogenic bacterial in flora were subjected to analysis. Im generalised infections Stafphylococcus epidermidis makes 56.6% and E. Coli accounts for 87.5 of the infections of the urinary system. In our research the late sepsis and pneumonia are more frequently the result of the hospital infection (14.2%) in the cases of congenital infections-pneumonia and the infection of the urinary system (72%). Hematologic indicators such as: leucopenia, thormbocytopenia, I/T are distinct infection markers (those were found in 31% of the cases). The CRP protein shows the lowest values in congenital infections, still monitoring its level is useful for assessing the effectiveness of the undertaken antybacterial treatment. The newborns of male sex (58%) more often prone to infection. Pneumonia is the manifestation pertaining to an organ in 70% of congenital infections, the infection of urinary system amounts to 17.1%.

Adhesive properties of P-like fimbriae in Klebsiella-species.

Clinical isolates of three encapsulated Klebsiella strains with type 1 (mannose-sensitive, MS+MR-), type 3 (mannose-resistant, MS-MR+), type 1.3 (MS+MR+) fimbriae and facultatively coexpressing P-like fimbria were investigated for their ability to adhere to uroepithelial cells (UECs) and tracheal epithelial cells (TECs). Irrespective of the type of epithelial cells, adhesion of the MS+MR+ (type 1.3) fimbriated Klebsiella strain was significantly stronger than adhesion of strains carrying only type 1 (MS+MR-) or type 3 (MS-MR+) fimbriae. The coexpression of P-like fimbriae increased the adhesive properties of Klebsiella strains to UECs but not to TECs. Adhesion of P-like fimbriated Klebsiella strains to UECs was significantly inhibited in the presence of the P+ fimbriae-specific Gal alpha-4-Gal beta (galabiose). Such adhesion was unrelated to the coexpression of type 1, type 3 or type 1.3 fimbriae. However, adhesion to TECs was only moderately inhibited.