Assuntos
Camundongos Obesos/genética , Obesidade/genética , Animais , História do Século XX , CamundongosAssuntos
Alergia e Imunologia/história , Animais , Antígenos H-2 , História do Século XX , Estados UnidosAssuntos
Complexo Principal de Histocompatibilidade , Neoplasias Experimentais/imunologia , Alelos , Animais , Antígenos de Neoplasias/genética , Antígenos Virais/genética , Antígenos Virais de Tumores , Feminino , Ligação Genética , Genótipo , Antígenos H-2/genética , Heterozigoto , Masculino , Camundongos , Camundongos Endogâmicos , Neoplasias Experimentais/genética , Linhagem , Ratos , Especificidade da EspécieAssuntos
Imunogenética , Complexo Principal de Histocompatibilidade , Animais , Formação de Anticorpos , Linfócitos B/imunologia , Diferenciação Celular , Membrana Celular/imunologia , Genética Médica , Antígenos H-2/genética , Humanos , Imunidade Celular , Terapia de Imunossupressão , Isoantígenos/genética , Camundongos , Linfócitos T/imunologiaAssuntos
Antígenos de Histocompatibilidade/genética , Animais , Membrana Celular/imunologia , Genes , Genes MHC da Classe II , Rejeição de Enxerto , Antígenos H-2/genética , Humanos , Imunidade Celular , Fígado/imunologia , Complexo Principal de Histocompatibilidade , Camundongos , Camundongos Endogâmicos/genética , Ratos , Pele/imunologia , Transplante de Pele , Linfócitos T/imunologiaAssuntos
Antígenos de Histocompatibilidade , Histocompatibilidade , Linfócitos T/imunologia , Animais , Sítios de Ligação de Anticorpos , Citotoxicidade Imunológica , Epitopos , Humanos , Imunoglobulinas , Memória Imunológica , Ativação Linfocitária , Cooperação Linfocítica , Macrófagos/imunologia , Viroses/imunologiaRESUMO
Seven congenic strains differing from C57BL/10Sn at theH-13 locus have been produced which define fourH-13 alleles. Isografting, exchanging of grafts between sublines, F(1) testing, and linkage testing demonstrate the presence of additionalH genes in four of these strains. The medial survival times (MSTs) of skin grafts fromH-13(a) to unimmunizedH-13(b) recipients ranged from 69 to 83 days. Rejection across all other barriers was extremely weak with most MSTs being > 100 days. Preinjection of donor strain thymocytes caused accelerated rejection of skin grafts fromH-13(a) toH-13(b) mice, but had only minimal effect on skin grafts across other barriers. Rejection ofH-13 incompatible grafts was significantly stronger when the donor and host areH-3(a) than when they wereH-3(b).
Assuntos
Linfócitos B/imunologia , Isoantígenos/análise , Linfócitos T/imunologia , Animais , Formação de Anticorpos , Testes Imunológicos de Citotoxicidade , Epitopos , Camundongos , Camundongos Endogâmicos AKR , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C3H , Camundongos Endogâmicos C57BLAssuntos
Antígenos de Histocompatibilidade , Memória Imunológica , Animais , Células Produtoras de Anticorpos , Linfócitos B/imunologia , Células da Medula Óssea , Transplante de Medula Óssea , Mapeamento Cromossômico , Genes , Células Híbridas , Imunidade Celular , Teste de Cultura Mista de Linfócitos , Camundongos , Linfócitos T/imunologia , Transplante HomólogoRESUMO
The Ly-4 locus defines an alloantigenic specificity present on the surface of lymphocytes of some mouse strains and detected in lymphocytotoxicity tests by an antiserum (BALB/c times SWR)F1 anti-B10.D2. In this paper are presented results which indicate that Ly-4.2 is predominantly represented on B cells and not T cells. In cytotoxicity tests with rabbit complement, the distribution of Ly-4.2 is restricted: thymus 5%, TDL 12%, spleen 60%, lymph node 30%, bone marrow 36%, Peyer's patches 55%, peritoneal exudate cells 35%, and peripheral lymphocytes 35%. This distribution is the reciprocal of that obtained with anti-Thy-1 antiserum. Further, when both reagents were used together, almost all cells in these tissues were lysed, excepting those with bone marrow. When anti-Ly-4.2 and anti-Thy-1.2 were used sequentially, it was apparent that these specificities were present on different cell populations. As Thy-1 is a marker for T cells, Ly-4 is therefore, presumably, a marker for B cells. This conclusion was confirmed by testing mice depleted of T cells ("nude"; thymectomy + anti-lymphocyte serum treatment; thymectomy, irradiation + reconstitution with bone marrow) where the majority of lymphocytes were Ly-4.2+, Thy-1.2-; and by testing tumors, where, for the most part, this reciprocal relationship held true. Appropriate studies indicate that the Ly-4.2 antibody does not detect a surface immunoglobulin allotype. Anti-Ly-4.2 anti-serum may provide a useful alloantigenic marker distinct from immunoglobulin, mouse-specific bone marrow-derived lymphocyte anti;en, and "beta" for detecting some or all, B cells.
Assuntos
Linfócitos B/imunologia , Isoantígenos , Animais , Especificidade de Anticorpos , Reações Antígeno-Anticorpo , Soro Antilinfocitário , Medula Óssea/imunologia , Células da Medula Óssea , Membrana Celular/imunologia , Radioisótopos de Cromo , Proteínas do Sistema Complemento , Testes Imunológicos de Citotoxicidade , Genótipo , Isoanticorpos , Linfonodos/citologia , Camundongos , Neoplasias Experimentais/imunologia , Nódulos Linfáticos Agregados/citologia , Timo/citologia , Timo/imunologia , Azul TripanoAssuntos
Especificidade de Anticorpos , Antígenos de Histocompatibilidade , Transplante de Pele , Animais , Testes Imunológicos de Citotoxicidade , Rejeição de Enxerto , Testes de Hemaglutinação , Camundongos , Camundongos Endogâmicos C3H , Camundongos Endogâmicos DBA , Fatores de Tempo , Sobrevivência de Tecidos , Transplante HomólogoAssuntos
Reações Cruzadas , Testes Imunológicos de Citotoxicidade , Antígenos de Histocompatibilidade , Animais , Formação de Anticorpos , Complexo Antígeno-Anticorpo , Reações Antígeno-Anticorpo , Epitopos , Haploidia , Testes de Hemaglutinação , Soros Imunes , Camundongos , Camundongos Endogâmicos , Mutação , Recombinação GenéticaRESUMO
The immunogenicity of single, private H-2 specificities has been tested. In most cases, the specificity was known to be confined either to H-2K or to H-2D. This was accomplished by the appropriate choice, as donor and recipient, or parent of the F(1) hybrid recipient, of congenic strains differing at H-2 only, and of recombinant haplotypes in donor and/or recipient. By nearly all tests, the H-2K antigen appeared to be a stronger immunogen than the H-2D antigen. Skin grafts with an H-2K difference showed median survival times (MST) of 9.3-14.5 days; for H-2D the values were 13.8-18.6. The difference was also present, though narrowed, for second-set grafts. H-2K grafts regularly engendered a demonstrable cytotoxic antibody response; with H-2D differences the response was absent or very weak. K end cytotoxic titers after multiple immunizations with lymphoid tissues ranged from 1/32 to 1/2,048, D end titers from 0 to 1/128. Hemagglutination titers showed no clear difference. The results of passive enhancement of skin grafts with H-2 alloantibody produced in donor recipient combinations, identical to those used for the skin grafts showed a different pattern. H-2K, despite its greater immunogenic strength was more easily enhanced than H-2D. Prolongation of MST's for H-2K was 2.4-6.7 days, for H-2D grafts, 0.2-1.5 days.
Assuntos
Antígenos de Histocompatibilidade , Animais , Formação de Anticorpos , Troca Genética , Testes Imunológicos de Citotoxicidade , Epitopos , Feminino , Rejeição de Enxerto , Testes de Hemaglutinação , Histocompatibilidade , Soros Imunes , Imunização Passiva , Injeções Intraperitoneais , Isoanticorpos/administração & dosagem , Masculino , Camundongos , Camundongos Endogâmicos , Transplante de Pele , Imunologia de Transplantes , Transplante HomólogoRESUMO
A new locus is described that determines an alloantigen on the surface of lymphocytes. It differs from loci previously described in that the corresponding antibody, at least in most antisera, reacts almost exclusively with node lymphocytes, and weakly or not at all with thymuslymphocytes. Positive strains include C57BL/6, C57BL/10, C57L, C57BR/cd, and RF; negative strains include BALB/c, C3H, and SJL. The symbol Ly-4 is assigned, with the C57BL/6 allele being Ly-4(b), and the BALB/c allele Ly-4(a).
