The 13CO-rich atmosphere of a young accreting super-Jupiter.

Isotope abundance ratios have an important role in astronomy and planetary sciences, providing insights into the origin and evolution of the Solar System, interstellar chemistry and stellar nucleosynthesis1,2. In contrast to deuterium/hydrogen ratios, carbon isotope ratios are found to be roughly constant (around 89) in the Solar System1,3, but do vary on galactic scales with a 12C/13C isotopologue ratio of around 68 in the current local interstellar medium4-6. In molecular clouds and protoplanetary disks, 12CO/13CO ratios can be altered by ice and gas partitioning7, low-temperature isotopic ion-exchange reactions8 and isotope-selective photodissociation9. Here we report observations of 13CO in the atmosphere of the young, accreting super-Jupiter TYC 8998-760-1 b, at a statistical significance of more than six sigma. Marginalizing over the planet's atmospheric temperature structure, chemical composition and spectral calibration uncertainties suggests a 12CO/13CO ratio of [Formula: see text](90% confidence), a substantial enrichment in 13C with respect to the terrestrial standard and the local interstellar value. As the current location of TYC 8998-760-1 b at greater than or equal to 160 astronomical units is far beyond the CO snowline, we postulate that it accreted a substantial fraction of its carbon from ices enriched in 13C through fractionation.

Citizen science with colour blindness: A case study on the Forel-Ule scale.

Many citizen science projects depend on colour vision. Examples include classification of soil or water types and biological monitoring. However, up to 1 in 11 participants are colour blind. We simulate the impact of various forms of colour blindness on measurements with the Forel-Ule scale, which is used to measure water colour by eye with a 21-colour scale. Colour blindness decreases the median discriminability between Forel-Ule colours by up to 33% and makes several colour pairs essentially indistinguishable. This reduces the precision and accuracy of citizen science data and the motivation of participants. These issues can be addressed by including uncertainty estimates in data entry forms and discussing colour blindness in training materials. These conclusions and recommendations apply to colour-based citizen science in general, including other classification and monitoring activities. Being inclusive of the colour blind increases both the social and scientific impact of citizen science.

Detection of polarization neutral points in observations of the combined corona and sky during the 21 August 2017 total solar eclipse.

We report the results of polarimetric observations of the total solar eclipse of 21 August 2017 from Rexburg, Idaho (USA). We use three synchronized DSLR cameras with polarization filters oriented at 0°, 60°, and 120° to provide high-dynamic-range RGB polarization images of the corona and surrounding sky. We measure tangential coronal polarization and vertical sky polarization, both as expected. These observations provide detailed detections of polarization neutral points above and below the eclipsed Sun where the coronal polarization is canceled by the sky polarization. We name these special polarization neutral points after Minnaert and Van de Hulst.

Standardized spectral and radiometric calibration of consumer cameras.

Consumer cameras, particularly onboard smartphones and UAVs, are now commonly used as scientific instruments. However, their data processing pipelines are not optimized for quantitative radiometry and their calibration is more complex than that of scientific cameras. The lack of a standardized calibration methodology limits the interoperability between devices and, in the ever-changing market, ultimately the lifespan of projects using them. We present a standardized methodology and database (SPECTACLE) for spectral and radiometric calibrations of consumer cameras, including linearity, bias variations, read-out noise, dark current, ISO speed and gain, flat-field, and RGB spectral response. This includes golden standard ground-truth methods and do-it-yourself methods suitable for non-experts. Applying this methodology to seven popular cameras, we found high linearity in RAW but not JPEG data, inter-pixel gain variations >400% correlated with large-scale bias and read-out noise patterns, non-trivial ISO speed normalization functions, flat-field correction factors varying by up to 2.79 over the field of view, and both similarities and differences in spectral response. Moreover, these results differed wildly between camera models, highlighting the importance of standardization and a centralized database.

Circular Spectropolarimetric Sensing of Vegetation in the Field: Possibilities for the Remote Detection of Extraterrestrial Life.

Homochirality is a generic and unique property of all biochemical life, and the fractional circular polarization of light it induces therefore constitutes a potentially unambiguous biosignature. However, while high-quality circular polarimetric spectra can be easily and quickly obtained in the laboratory, accurate measurements in the field are much more challenging due to large changes in illumination and target movement. In this study, we measured various targets in the field, up to distances of a few kilometers, using the dedicated circular spectropolarimeter TreePol. We show how photosynthetic life can readily be distinguished from abiotic matter. We underline the potential of circular polarization signals as a remotely accessible means to characterize and monitor terrestrial vegetation, for example, for agriculture and forestry. In addition, we discuss the potential of circular polarization for the remote detection of extraterrestrial life.

Simultaneous phase and amplitude aberration sensing with a liquid-crystal vector-Zernike phase mask.

We present an enhanced version of the Zernike wavefront sensor (WFS), which simultaneously measures phase and amplitude aberrations. The "vector-Zernike" WFS consists of a patterned liquid-crystal mask, which imposes a ±π/2 phase on the point spread function core through the achromatic geometric phase acting with the opposite sign on opposite circular polarizations. After splitting circular polarization, the ensuing pupil intensity images are used to reconstruct the phase and the amplitude of the incoming wavefront. We demonstrate reconstruction of the complex wavefront with monochromatic lab measurements and show in simulation the high accuracy and sensitivity over a bandwidth up to 100%.

Circular spectropolarimetric sensing of higher plant and algal chloroplast structural variations.

Photosynthetic eukaryotes show a remarkable variability in photosynthesis, including large differences in light-harvesting proteins and pigment composition. In vivo circular spectropolarimetry enables us to probe the molecular architecture of photosynthesis in a non-invasive and non-destructive way and, as such, can offer a wealth of physiological and structural information. In the present study, we have measured the circular polarizance of several multicellular green, red, and brown algae and higher plants, which show large variations in circular spectropolarimetric signals with differences in both spectral shape and magnitude. Many of the algae display spectral characteristics not previously reported, indicating a larger variation in molecular organization than previously assumed. As the strengths of these signals vary by three orders of magnitude, these results also have important implications in terms of detectability for the use of circular polarization as a signature of life.

Imaging linear and circular polarization features in leaves with complete Mueller matrix polarimetry.

Spectropolarimetry of intact plant leaves allows to probe the molecular architecture of vegetation photosynthesis in a non-invasive and non-destructive way and, as such, can offer a wealth of physiological information. In addition to the molecular signals due to the photosynthetic machinery, the cell structure and its arrangement within a leaf can create and modify polarization signals. Using Mueller matrix polarimetry with rotating retarder modulation, we have visualized spatial variations in polarization in transmission around the chlorophyll a absorbance band from 650 nm to 710 nm. We show linear and circular polarization measurements of maple leaves and cultivated maize leaves and discuss the corresponding Mueller matrices and the Mueller matrix decompositions, which show distinct features in diattenuation, polarizance, retardance and depolarization. Importantly, while normal leaf tissue shows a typical split signal with both a negative and a positive peak in the induced fractional circular polarization and circular dichroism, the signals close to the veins only display a negative band. The results are similar to the negative band as reported earlier for single macrodomains. We discuss the possible role of the chloroplast orientation around the veins as a cause of this phenomenon. Systematic artefacts are ruled out as three independent measurements by different instruments gave similar results. These results provide better insight into circular polarization measurements on whole leaves and options for vegetation remote sensing using circular polarization.

Citizen science on a smartphone: Participants' motivations and learning.

Citizen science provides researchers means to gather or analyse large datasets. At the same time, citizen science projects offer an opportunity for non-scientists to be part of and learn from the scientific process. In the Dutch iSPEX project, a large number of citizens turned their smartphones into actual measurement devices to measure aerosols. This study examined participants' motivation and perceived learning impacts of this unique project. Most respondents joined iSPEX because they wanted to contribute to the scientific goals of the project or because they were interested in the project topics (health and environmental impact of aerosols). In terms of learning impact, respondents reported a gain in knowledge about citizen science and the topics of the project. However, many respondents had an incomplete understanding of the science behind the project, possibly caused by the complexity of the measurements.

Spectral line polarimetry with a channeled polarimeter.

Channeled spectropolarimetry or spectral polarization modulation is an accurate technique for measuring the continuum polarization in one shot with no moving parts. We show how a dual-beam implementation also enables spectral line polarimetry at the intrinsic resolution, as in a classic beam-splitting polarimeter. Recording redundant polarization information in the two spectrally modulated beams of a polarizing beam-splitter even provides the possibility to perform a postfacto differential transmission correction that improves the accuracy of the spectral line polarimetry. We perform an error analysis to compare the accuracy of spectral line polarimetry to continuum polarimetry, degraded by a residual dark signal and differential transmission, as well as to quantify the impact of the transmission correction. We demonstrate the new techniques with a blue sky polarization measurement around the oxygen A absorption band using the groundSPEX instrument, yielding a polarization in the deepest part of the band of 0.160±0.010, significantly different from the polarization in the continuum of 0.2284±0.0004. The presented methods are applicable to any dual-beam channeled polarimeter, including implementations for snapshot imaging polarimetry.

Performance characterization of a broadband vector Apodizing Phase Plate coronagraph.

One of the main challenges for the direct imaging of planets around nearby stars is the suppression of the diffracted halo from the primary star. Coronagraphs are angular filters that suppress this diffracted halo. The Apodizing Phase Plate coronagraph modifies the pupil-plane phase with an anti-symmetric pattern to suppress diffraction over a 180 degree region from 2 to 7 λ/D and achieves a mean raw contrast of 10(-4) in this area, independent of the tip-tilt stability of the system. Current APP coronagraphs implemented using classical phase techniques are limited in bandwidth and suppression region geometry (i.e. only on one side of the star). In this paper, we introduce the vector-APP (vAPP) whose phase pattern is implemented through the vector phase imposed by the orientation of patterned liquid crystals. Beam-splitting according to circular polarization states produces two, complementary PSFs with dark holes on either side. We have developed a prototype vAPP that consists of a stack of three twisting liquid crystal layers to yield a bandwidth of 500 to 900 nm. We characterize the properties of this device using reconstructions of the pupil-plane pattern, and of the ensuing PSF structures. By imaging the pupil between crossed and parallel polarizers we reconstruct the fast axis pattern, transmission, and retardance of the vAPP, and use this as input for a PSF model. This model includes aberrations of the laboratory set-up, and matches the measured PSF, which shows a raw contrast of 10(-3.8) between 2 and 7 λ/D in a 135 degree wedge. The vAPP coronagraph is relatively easy to manufacture and can be implemented together with a broadband quarter-wave plate and Wollaston prism in a pupil wheel in high-contrast imaging instruments. The liquid crystal patterning technique permits the application of extreme phase patterns with deeper contrasts inside the dark holes, and the multilayer liquid crystal achromatization technique enables unprecedented spectral bandwidths for phase-manipulation coronagraphy.

Compact and robust method for full Stokes spectropolarimetry.

We present an approach to spectropolarimetry that requires neither moving parts nor time dependent modulation, and that offers the prospect of achieving high sensitivity. The technique applies equally well, in principle, in the optical, UV, or IR. The concept, which is one of those generically known as channeled polarimetry, is to encode the polarization information at each wavelength along the spatial dimension of a two-dimensional data array using static, robust optical components. A single 2D data frame contains the full polarization information and can be configured to measure either two or all of the Stokes polarization parameters. By acquiring full polarimetric information in a single observation, we simplify polarimetry of transient sources and in situations where the instrument and target are in relative motion. The robustness and simplicity of the approach, coupled with its potential for high sensitivity, and applicability over a wide wavelength range, is likely to prove useful for applications in challenging environments such as space.

Spectral modulation for full linear polarimetry.

Linear (spectro) polarimetry is usually performed using separate photon flux measurements after spatial or temporal polarization modulation. Such classical polarimeters are limited in sensitivity and accuracy by systematic effects and noise. We describe a spectral modulation principle that is based on encoding the full linear polarization properties of light in its spectrum. Such spectral modulation is obtained with an optical train of an achromatic quarter-wave retarder, an athermal multiple-order retarder, and a polarizer. The emergent spectral modulation is sinusoidal with its amplitude scaling with the degree of linear polarization and its phase scaling with the angle of linear polarization. The large advantage of this passive setup is that all polarization information is, in principle, contained in a single spectral measurement, thereby eliminating all differential effects that potentially create spurious polarization signals. Since the polarization properties are obtained through curve fitting, the susceptibility to noise is relatively low. We provide general design options for a spectral modulator and describe the design of a prototype modulator. Currently, the setup in combination with a dedicated retrieval algorithm can be used to measure linear polarization signals with a relative accuracy of 5%.