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1.
Neuropsychopharmacology ; 40(11): 2517-25, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25865931

RESUMO

The stress-related neuropeptide, corticotropin-releasing factor (CRF) regulates the dorsal raphe nucleus-serotonin (DRN-5-HT) system during stress and this may underlie affective and cognitive dysfunctions that characterize stress-related psychiatric disorders. CRF acts on both CRF1 and CRF2 receptor subtypes in the DRN that exert opposing inhibitory and excitatory effects on DRN-5-HT neuronal activity and 5-HT forebrain release, respectively. The current study first assessed the cognitive effects of intra-DRN microinfusion of CRF or the selective CRF2 agonist, urocortin II in stress-naive rats on performance of an operant strategy set-shifting task that is mediated by the medial prefrontal cortex (mPFC). CRF (30 ng) facilitated strategy set-shifting performance, whereas higher doses of CRF and urocortin II that would interact with CRF2 were without effect, consistent with a CRF1-mediated action. This dose decreased 5-HT extracellular levels in the mPFC, further supporting a role for CRF1. The effects of CRF were then assessed in rats exposed to repeated social stress using the resident-intruder model. Repeated social stress shifted the CRF effect from facilitation of strategy set shifting to facilitation of reversal learning and this was most prominent in a subpopulation of rats that resist defeat. Notably, in this subpopulation of rats 5-HT neuronal responses to CRF have been demonstrated to shift from CRF1-mediated inhibition to CRF2-mediated excitation. Because 5-HT facilitates reversal learning, the present results suggest that stress-induced changes in the cellular effects of CRF in the DRN translate to changes in cognitive effects of CRF. Together, the results underscore the potential for stress history to shift cognitive processing through changes in CRF neurotransmission in the DRN and the association of this effect with coping strategy.


Assuntos
Cognição/fisiologia , Hormônio Liberador da Corticotropina/metabolismo , Núcleo Dorsal da Rafe/metabolismo , Função Executiva/fisiologia , Reversão de Aprendizagem/fisiologia , Estresse Psicológico/metabolismo , Adaptação Psicológica/efeitos dos fármacos , Adaptação Psicológica/fisiologia , Animais , Fármacos do Sistema Nervoso Central/administração & dosagem , Cognição/efeitos dos fármacos , Condicionamento Operante/efeitos dos fármacos , Condicionamento Operante/fisiologia , Hormônio Liberador da Corticotropina/administração & dosagem , Dominação-Subordinação , Dopamina/metabolismo , Núcleo Dorsal da Rafe/efeitos dos fármacos , Função Executiva/efeitos dos fármacos , Masculino , Ratos Long-Evans , Ratos Sprague-Dawley , Receptores de Hormônio Liberador da Corticotropina/agonistas , Receptores de Hormônio Liberador da Corticotropina/metabolismo , Reversão de Aprendizagem/efeitos dos fármacos , Serotonina/metabolismo , Urocortinas/administração & dosagem , Urocortinas/metabolismo
2.
Psychopharmacology (Berl) ; 232(1): 185-95, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24958230

RESUMO

RATIONALE: Stress experience during adolescence has been linked to the development of psychiatric disorders in adulthood, many of which are associated with impairments in prefrontal cortex function. OBJECTIVE: The current study was designed to determine the immediate and enduring effects of repeated social stress on a prefrontal cortex-dependent cognitive task. METHODS: Early adolescent (P28), mid-adolescent (P42), and adult (P70) rats were exposed to resident-intruder stress for 5 days and tested in an operant strategy-shifting task (OSST) during the following week or several weeks later during adulthood. Engagement of prefrontal cortical neurons during the task was assessed by expression of the immediate early gene, c-fos. RESULTS: Social stress during adolescence had no immediate effects on task performance, but impaired strategy-shifting in adulthood, whereas social stress that occurred during adulthood had no effect. The cognitive impairment produced by adolescent social stress was most pronounced in rats with a passive coping strategy. Notably, strategy-shifting performance was positively correlated with medial prefrontal cortical c-fos in adulthood but not in adolescence, suggesting that the task engages different brain regions in adolescents compared to adults. CONCLUSIONS: Adolescent social stress produces a protracted impairment in prefrontal cortex-mediated cognition that is related to coping strategy. This impairment may be selectively expressed in adulthood because prefrontal cortical activity is integral to task performance at this age but not during adolescence.


Assuntos
Adaptação Psicológica/fisiologia , Encéfalo/crescimento & desenvolvimento , Encéfalo/metabolismo , Cognição/fisiologia , Comportamento Social , Estresse Psicológico/metabolismo , Adolescente , Adulto , Fatores Etários , Animais , Humanos , Masculino , Córtex Pré-Frontal/metabolismo , Ratos , Ratos Long-Evans , Ratos Sprague-Dawley , Estresse Psicológico/psicologia
3.
PLoS One ; 6(3): e18264, 2011 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-21483826

RESUMO

Sensory experience, and the lack thereof, can alter the function of excitatory synapses in the primary sensory cortices. Recent evidence suggests that changes in sensory experience can regulate the synaptic level of Ca(2+)-permeable AMPA receptors (CP-AMPARs). However, the molecular mechanisms underlying such a process have not been determined. We found that binocular visual deprivation, which is a well-established in vivo model to produce multiplicative synaptic scaling in visual cortex of juvenile rodents, is accompanied by an increase in the phosphorylation of AMPAR GluR1 (or GluA1) subunit at the serine 845 (S845) site and the appearance of CP-AMPARs at synapses. To address the role of GluR1-S845 in visual deprivation-induced homeostatic synaptic plasticity, we used mice lacking key phosphorylation sites on the GluR1 subunit. We found that mice specifically lacking the GluR1-S845 site (GluR1-S845A mutants), which is a substrate of cAMP-dependent kinase (PKA), show abnormal basal excitatory synaptic transmission and lack visual deprivation-induced homeostatic synaptic plasticity. We also found evidence that increasing GluR1-S845 phosphorylation alone is not sufficient to produce normal multiplicative synaptic scaling. Our study provides concrete evidence that a GluR1 dependent mechanism, especially S845 phosphorylation, is a necessary pre-requisite step for in vivo homeostatic synaptic plasticity.


Assuntos
Plasticidade Neuronal/fisiologia , Receptores de AMPA/metabolismo , Privação Sensorial/fisiologia , Sinapses/metabolismo , Animais , Eletroforese em Gel de Poliacrilamida , Immunoblotting , Técnicas In Vitro , Camundongos , Plasticidade Neuronal/genética , Fosforilação , Receptores de AMPA/genética
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