RESUMO
7,10-Dihydroxy-8(E)-octadecenoic acid (DOD), one of hydroxy fatty acids, was successfully produced from oleic acid and natural vegetable oils containing oleic acid by a bacterial strain Pseudomonas aeruginosa (PR3). However, biological properties of DOD remained unknown so far. In this study, as a trial to determine the biological properties of DOD molecule, antibacterial activities of DOD against plant pathogenic bacteria were determined qualitatively and quantitatively. DOD presented strong antibacterial activities against all the bacterial strains tested with MIC value being in the range of 125-1000µg/ml and there was no sensitivity preference detected between Gram-positive and Gram-negative strains.
Assuntos
Antibacterianos/farmacologia , Bactérias/efeitos dos fármacos , Ácidos Oleicos/farmacologia , Plantas/microbiologia , Antibacterianos/biossíntese , Bactérias/patogenicidade , Biotecnologia , Corynebacterium glutamicum/efeitos dos fármacos , Corynebacterium glutamicum/patogenicidade , Testes de Sensibilidade a Antimicrobianos por Disco-Difusão , Erwinia/efeitos dos fármacos , Erwinia/patogenicidade , Testes de Sensibilidade Microbiana , Ácido Oleico/metabolismo , Ácidos Oleicos/biossíntese , Pseudomonas/efeitos dos fármacos , Pseudomonas/patogenicidade , Pseudomonas aeruginosa/metabolismo , Pseudomonas syringae/efeitos dos fármacos , Pseudomonas syringae/patogenicidade , Ralstonia solanacearum/efeitos dos fármacos , Ralstonia solanacearum/patogenicidade , Especificidade da EspécieRESUMO
Microbial conversions of unsaturated fatty acids often generate polyhydroxy fatty acids, giving them new properties such as higher viscosity and reactivity. A bacterial strain Pseudomonas aeruginosa (PR3) has been intensively studied to produce mono-, di-, and trihydroxy fatty acids from different 9-cis-monoenoic fatty acids such as oleic acid, ricinoleic acid, and palmitoleic acid. However, from the results and the postulated similar metabolic pathways involved in these transformations, it was assumed that the enzyme system involved in transformation of the monoenoic fatty acid by strain PR3 could utilize fatty acids with different chain lengths and locations of the double bond. In this study was used as a substrate for bioconversion by strain PR3 eicosenoic acid (C20:1, ω-9) containing a singular cis double bond at different positions from the carboxyl end as oleic acid, and it was confirmed that PR3 could produce a novel 9,12-dihydroxy-10(E)-eicosenoic acid (DED) with 6.2% yield from eicosenoic acid. The structure of DED was confirmed using GC-MS, FTIR, and NMR analyses. DED production was maximized at 72 h after the substrate was added to the 24 h culture. Some other nutritional factors were also studied for optimal production of DED.
