Evaluation of various energy windows at different radionuclides for scatter and attenuation correction in nuclear medicine.

OBJECTIVE: Improving signal to noise ratio (SNR) and qualified images by the various methods is very important for detecting the abnormalities at the body organs. Scatter and attenuation of photons by the organs lead to errors in radiopharmaceutical estimation as well as degradation of images. The choice of suitable energy window and the radionuclide have a key role in nuclear medicine which appearing the lowest scatter fraction as well as having a nearly constant linear attenuation coefficient as a function of phantom thickness. METHODS: The energy windows of symmetrical window (SW), asymmetric window (ASW), high window (WH) and low window (WL) using Tc-99m and Sm-153 radionuclide with solid water slab phantom (RW3) and Teflon bone phantoms have been compared, and Matlab software and Monte Carlo N-Particle (MCNP4C) code were modified to simulate these methods and obtaining the amounts of FWHM and full width at tenth maximum (FWTM) using line spread functions (LSFs). The experimental data were obtained from the Orbiter Scintron gamma camera. RESULTS: Based on the results of the simulation as well as experimental work, the performance of WH and ASW display of the results, lowest scatter fraction as well as constant linear attenuation coefficient as a function of phantom thickness. WH and ASW were optimal windows in nuclear medicine imaging for Tc-99m in RW3 phantom and Sm-153 in Teflon bone phantom. Attenuation correction was done for WH and ASW optimal windows and for these radionuclides using filtered back projection algorithm. Results of simulation and experimental show that very good agreement between the set of experimental with simulation as well as theoretical values with simulation data were obtained which was nominally less than 7.07 % for Tc-99m and less than 8.00 % for Sm-153. Corrected counts were not affected by the thickness of scattering material. The Simulated results of Line Spread Function (LSF) for Sm-153 and Tc-99m in phantom based on four windows and TEW method were indicated that the FWHM and FWTM values were approximately the same in TEW method and WH and ASW, but the sensitivity at the optimal window was more than that of the other one. CONCLUSIONS: The suitable determination of energy window width on the energy spectra can be useful in optimal design to improve efficiency and contrast. It is found that the WH is preferred to the ASW and the ASW is preferred to the SW.

A comprehensive procedure for characterizing arbitrary azimuthally symmetric photon beams.

PURPOSE: A new Monte Carlo (MC) source model (SM) has been developed for azimuthally symmetric photon beams. METHODS: The MC simulation tallied phase space file (PSF) is divided into two categories depending on the relationship of the particle track line to the beam central axis: multiple point source (MPS) and spatial mesh based surface source (SMBSS). To validate this SM, MCNPX2.6 was used to generate two PSFs for a 6 MV photon beam from a Varian 2100C/D linear accelerator. RESULTS: PDDs and profiles were calculated using the SM and original PSF for different field sizes from 5 × 5 to 40 × 40 cm2. Agreement was within 2% of the maximum dose at 100 cm SSD and 2.5% of the maximum dose at 200 cm SSD for beam profiles at depths of 3.5 cm and 15 cm with respect to the original PSF. Differences between the source model and the PSF in the out-of-field regions were less than 0.5% of the profile maximum value at 100 cm SSD. Differences between measured and calculated points were less than 2% of the maximum dose or 2 mm distance to agreement (DTA) at 100 cm SSD. CONCLUSIONS: This SM is unique in that it accounts for a higher level of energy dependence on the particle's direction and it is independent from accelerator components, unlike other published SMs. The model can be applied to any arbitrary azimuthally symmetric beam and has source biasing capabilities that significantly increase the simulation speed up to 3300 for certain field sizes.

Down-regulation of Hsp27 radiosensitizes human prostate cancer cells.

AIM: In this study, we examined whether human Hsp27 is involved in the radiation sensitization induced by gamma radiation in cultured human prostate cancer cells. METHODS: We transfected DU145 cells with full length Hsp27 antisense cDNA to obtain viable cell lines which expressed reduced Hsp27. Selected individual clones were subjected to western blot analyses to confirm reduced expression of Hsp27. RESULTS: Hsp27 belongs to a family of abundant and ubiquitous stress proteins, the small heat shock proteins. It has been shown that Hsp27 can inhibit apoptosis both in a caspase-dependent and independent manner. Colony assays showed that the cells engineered to express reduced Hsp27 levels had a significantly increased sensitivity to gamma radiation compared with control cells that were transfected with the vector alone. However, there was also a significant difference in viability of cells with reduced Hsp27 levels and control cells 72 h after gamma-irradiation. CONCLUSIONS: Our results suggest the possible application of antisense Hsp27 cDNA or other methods to reduce Hsp27 expression as a radiation sensitizer in radiation oncology.