Assuntos
4-Butirolactona/análogos & derivados , Ácidos Graxos/metabolismo , Substâncias de Crescimento/biossíntese , Streptomyces griseus/metabolismo , 4-Butirolactona/biossíntese , 4-Butirolactona/química , 4-Butirolactona/genética , Substâncias de Crescimento/química , Substâncias de Crescimento/genética , Streptomyces griseus/genética , Streptomyces griseus/crescimento & desenvolvimentoRESUMO
The PMR 1H spectra of aklavinone, monoanhydroaklavinone and bisanhydroaklavinone were completely assigned with the use of the double resonance technique and the method of resolution enhancement (multiplication of the free induction decay by the Gauss function). It was shown that under mild conditions in the presence of hydrazine, aklavinone dehydrated at the C9-C10 bond and transformed into monoanhydroaklavinone. Under less mild conditions when akalavinone was heated at 250 degrees C, dehydration proceeded at the C9-C10 and C7-C8 bonds to form bisanhydroaklavinone.
Assuntos
Antraciclinas , Antibióticos Antineoplásicos , Fenômenos Químicos , Química , Temperatura Alta , Espectroscopia de Ressonância Magnética , Naftacenos/análiseRESUMO
Mutant 170 not capable of forming streptidine and streptomycin was obtained using chemical mutagenes. This mutant can produce streptomycin only with suplementation of exogenous streptidine. Experiment with labeled C14-streptidine showed its specific incorporation in streptidine moiety of streptomycin molecule.
