Cell damage produced by magnetic fluid hyperthermia on microglial BV2 cells.

We present evidence on the effects of exogenous heating by water bath (WB) and magnetic hyperthermia (MHT) on a glial micro-tumor phantom. To this, magnetic nanoparticles (MNPs) of 30-40 nm were designed to obtain particle sizes for maximum heating efficiency. The specific power absorption (SPA) values (f = 560 kHz, H = 23.9 kA/m) for as prepared colloids (533-605 W/g) dropped to 98-279 W/g in culture medium. The analysis of the intracellular MNPs distribution showed vesicle-trapped MNPs agglomerates spread along the cytoplasm, as well as large (~0.5-0.9 µm) clusters attached to the cell membrane. Immediately after WB and MHT (T = 46 °C for 30 min) the cell viability was ≈70% and, after 4.5 h, decreased to 20-25%, demonstrating that metabolic processes are involved in cell killing. The analysis of the cell structures after MHT revealed a significant damage of the cell membrane that is correlated to the location of MNPs clusters, while local cell damage were less noticeable after WB without MNPs. In spite of the similar thermal effects of WB and MHT on the cell viability, our results suggest that there is an additional mechanism of cell damage related to the presence of MNPs at the intracellular space.

Atención temprana y prácticas centradas en la familia: a propósito de un caso / Early intervention and family-centered practice: a case report

En atención temprana la evidencia científica pone de manifiesto la eficacia y eficiencia de las prácticas centradas en la familia y en entornos naturales. Las intervenciones con niños que padecen una discapacidad o se encuentran en una situación de riesgo deben permitir la participación de las familias y/o los profesores. Las prácticas que generan competencias en los cuidadores del niño son las que favorecen el desarrollo en sus rutinas y contextos habituales. En este trabajo presentamos el análisis de un caso que ejemplifica cómo los aspectos teóricos pueden trasladarse a la esfera de la práctica diaria. Se muestran los aspectos clave de la intervención con prácticas centradas en la familia y en entornos naturales siguiendo el modelo basado en rutinas. Se describen el plan individual de apoyo a la familia y los apoyos educativos de una niña desde su evaluación inicial, descripción de objetivos funcionales, plan de intervención y seguimiento. Como resultado de la intervención, se observa una mejora en el desarrollo funcional de la pequeña y tanto la familia como la profesora se sienten más competentes, empoderados y con mejor calidad de vida familiar. Queda reflejada la importancia del trabajo transdisciplinar, la colaboración entre diferentes agentes sociales y el indiscutible papel de la familia durante todo el proceso (AU)

Scientific evidence demonstrates in Early Intervention the effectiveness and efficiency of family-centered practices and natural environments. Interventions with children who have a disability or are at a risk should allow participation of families and/or teachers. The practices must generate skills in the child's caregivers to promote the development of their usual routines and contexts. This paper presents the analysis of a case that exemplifies how the theoretical aspects can be transferred to the sphere of daily practice. We use de Routines Based Model to shown the intervention. The Individual Family Support Plan describes the service: Initial assessment, functional objectives, intervention plan and monitoring. As a result of the intervention, we can see the improvement in the functional development and we observe the family and the teacher feel more competent, empowered and better family quality of family life. The importance of transdisciplinary work, collaboration between different social agents and the undisputed role of the family throughout the process is reflected (AU)