RESUMO
The present investigation attempted study the potential use of the Holothuria arenicola saponin (HaS) against Ehrlich Ascites Carcinoma (EAC) tumor model in female Swiss albino mice. The HPLC studies of the extracted saponin showed the peak at retention time 5.19 min. was is matched with the standard saponin with total amount 34.87%. Significant improvements were detected in the tumor markers, complete blood count, antioxidant system, liver function and kidney function of HaS-treated mice. The present study demonstrated that HaS is a potent natural product that has promising antineoplastic efficacy.
Assuntos
Antineoplásicos/uso terapêutico , Ascite/tratamento farmacológico , Carcinoma de Ehrlich/tratamento farmacológico , Holothuria/química , Saponinas/isolamento & purificação , Saponinas/uso terapêutico , Animais , Antineoplásicos/farmacologia , Antioxidantes/uso terapêutico , Linhagem Celular Tumoral , Feminino , Camundongos , Extratos Vegetais/uso terapêutico , Espécies Reativas de Oxigênio/metabolismo , Saponinas/farmacologiaRESUMO
BACKGROUND: Diabetes mellitus is the main reason of morbidity and mortality worldwide. In recent years great attention has been paid to bioactivity of natural products due to their potential pharmacological utilization. Echinochrome is a natural compound isolated from sea urchins and possesses many biological effects. The hypoglycemic activity of echinochrome reported in many recent experiments. OBJECTIVE: In our study, we tried to collect all the possible hypoglycemic mechanism of echinochrome. CONCLUSION: The hypoglycemic effect of echinochrome involving four main pathways; regeneration of pancreas, decrease insulin resistant, increase insulin production and improve glucose homeostasis.
Assuntos
Glicemia/efeitos dos fármacos , Diabetes Mellitus/tratamento farmacológico , Hipoglicemiantes/farmacologia , Resistência à Insulina , Naftoquinonas/farmacologia , Animais , Antioxidantes/farmacologia , Biomarcadores/sangue , Glicemia/metabolismo , Inibidores da Colinesterase/farmacologia , Diabetes Mellitus/sangue , Diabetes Mellitus/diagnóstico , Diabetes Mellitus/fisiopatologia , Glucosefosfato Desidrogenase/metabolismo , Heme Oxigenase-1/metabolismo , Hexoquinase/metabolismo , Homeostase , Humanos , Insulina/sangue , Fígado/efeitos dos fármacos , Fígado/metabolismo , Pâncreas/efeitos dos fármacos , Pâncreas/metabolismo , Pâncreas/fisiopatologia , Coativador 1-alfa do Receptor gama Ativado por Proliferador de Peroxissomo/metabolismo , Fosfatidilinositol 3-Quinase/metabolismo , Regeneração/efeitos dos fármacos , Transdução de Sinais/efeitos dos fármacosRESUMO
OBJECTIVE: The present investigation explored the therapeutic potential effect of Sepia officinalis body tissue (SOBT) and Sepia officinalis polysaccharide (SOP) extracts, in animal model of sepsis [induced by cecal ligation and puncture (CLP)]. MATERIALS AND METHODS: Experimental animals were divided into 4 groups, Group 1: Sham control rats. Group 2: Septic rats. Group 3: Septic rats treated with methanolic extract of Sepia officinalis body tissue (SOBT) (500mg/kg body weight) for 2days. Group 4: Septic rats treated with Sepia officinalis polysaccharide (SOP) extract (200mg/kg body weight) for 2days. RESULTS: The antioxidant activity of SOBT and SOP was proven by DPPH test. CLP-induced liver and kidney toxicities showed by an increase in the ALAT, ASAT, γGT, ALP, creatinine, BUN and uric acid concentrations in serum. Moreover, CLP-induced oxidative stress in liver and kidney evidenced by the increase of MDA levels, decrease in GSH concentrations and decrease in the enzymatic antioxidants (SOD, CAT, GST). In addition, CLP caused decrease in CYP1A2 content in liver. CONCLUSIONS: Our findings demonstrate the therapeutic efficacy of SOBT and SOP in liver and kidney disorders. Therefore this study suggests that SOBT and SOP could be a potential therapeutic agents for sepsis treatment.
Assuntos
Antioxidantes/farmacologia , Ceco/patologia , Modelos Animais de Doenças , Rim/patologia , Fígado/patologia , Sepia/química , Sepse/patologia , Animais , Ativação Enzimática , Peroxidação de Lipídeos , Testes de Função Hepática , Masculino , Estresse Oxidativo , Ratos , Ratos WistarRESUMO
BACKGROUND: Diabetes mellitus is one of the most public metabolic disorders. It is mainly classified into type 1 and type 2. Echinochrome is a pigment from sea urchins that has antioxidant, anti-microbial, anti-inflammatory and chelating abilities. AIMS: The present study aimed to investigate the anti-diabetic mechanisms of echinochrome pigment in streptozotocin-induced diabetic rats. MAIN METHODS: Thirty six male Wistar albino rats were divided into two main groups, type 1 diabetes and type 2 diabetes groups. Each group was divided into 3 subgroups (6 rats/subgroup); control, diabetic and echinochrome groups. Diabetic model was induced by a single dose of streptozotocin (60mg/kg, i.p) for type 1 diabetes and by a high fat diet for 4weeks before the injection with streptozotocin (30mg/kg, i.p) for type 2 diabetes. Diabetic groups were treated orally with echinochrome extract (1mg/kg body weight in 10% DMSO) daily for 4weeks. KEY FINDINGS: Echinochrome groups showed a reduction in the concentrations of glucose, MDA and the activities of arginase, AST, ALT, ALP and GGT. While it caused general increase in the levels of insulin, TB, DB, IB, NO and the activities of G6PD, GST, GPx, SOD and GSH. The histopathological investigation showed partial restoration of pancreatic islet cells and clear improvement in the hepatic architecture. SIGNIFICANCE: The suggested mechanism of Ech action in the reduction of diabetic complications in liver involved two pathways; through the hypoglycemic activity and the antioxidant role of Ech.
