RESUMO
Hydrogel matrices for cell cultivation have been generated by two-photon laser polymerization of unsaturated chitosan derivatives and methacrylated hyaluronic acid. The adhesive and toxic properties of the matrices have been assessed, and the matrices have been shown to have a good compatibility with primary hippocampal cell cultures. The formation of morphologically normal neural networks by cells of the nervous system cultured on the surface of hydrogel matrices has been observed. The metabolic status of dissociated hippocampal cells cultured on the matrices was similar to that of the control cultures, as shown by the results of MTT reductase activity assay. Thus, matrices based on unsaturated polysaccharide derivatives crosslinked by laser irradiation showed good compatibility with differentiated cells of the nervous system and considerable potential for use in neurotransplantation.
Assuntos
Quitosana , Hipocampo/metabolismo , Hidrogéis , Lasers , Teste de Materiais , Rede Nervosa/metabolismo , Animais , Quitosana/química , Quitosana/farmacologia , Hipocampo/citologia , Hidrogéis/síntese química , Hidrogéis/química , Hidrogéis/farmacologia , Camundongos , Rede Nervosa/citologiaRESUMO
Two comparable groups, 60 patients in each, were included in the study. Localization and area of the wounds, as well as basic treatment were equal in these groups. Patients of the main group received the additional photodynamic therapy with chlorine photosensibilizing agent. It led to the shortening of the wound clearing time and stimulation of the epithelisation together with fast decrease of bacterial load. Morphologic analysis showed faster inflammation decrease and granulation as well as lesser microcirculatory disorders in wounds treated with photodynamic therapy. Moreover, cicatrixes were more elastic and gentle in these patients.
