[Monitoring for Neisseria meningitidis species using sequencing of variable fragments of surface proteins FetA and PorA genes].

AIM: To perform advanced antigenic characterization of meningococci belonging to serogroups A and B and circulating in Moscow according to modern nomenclature of Neisseria meningitidis strains. MATERIALS AND METHODS: Method of typing of "VR" fragment of FetA protein together with methods of genetic subtyping and multilocus sequence typing was used. RESULTS: Detailed information about studied strains was inputed in Internet database--http://pubmlst.org/neisseria/. Typing of serogroup B strains did not allow to define dominating variant of "VR, fragment of FetA protein which is in accordance with subtyping data obtained previously. Serogroup A strains were notable for less variability of "VR" fragment variants: 6 variants were detected. For the majority of serogroup A strains, it was possible to trace connection between belonging of the strain to particular genetic subgroup and its revealed antigenic profile. For strains from genetic subgroup VI, antigenic profile P1.5-2, 10; F1-5 detected in 14(18%) strains was typical, whereas antigenic profile P1.5-2, 10; F3-5 was typical for genetic subgroup X and was detected in 50 (63%) strains. Antigenic profile P1.5-2, 10-67; F3-5 was detected in 5 (6%) strains, and other 10 antigenic profiles were revealed in one strain each. CONCLUSION: Prevalence of strains with antigenic profile P1.5-2, 10; F3-5 is explained by change of predominant genetic subgroup from subgroup VI to subgroup X in Moscow population serogroup A meningococci observed after 2003.

[Genetic subgroups of Neisseria meningitidis serogroup A isolated from patients with disseminated forms of meningococcal infection in Moscow, 1969-2006].

Results of microbiological monitoring for serogroup A Neisseria meningitidis circulated in Moscow from 2002 to 2006 are presented. Using multilocus sequence-typing, molecular and epidemiologic characteristics of 32 cultures isolated from cerebro-spinal fluid of patients with generalized forms of meningococcal infection. Typed isolates belonged to 4 sequence types: CT-3349 (detected in 24 cultures), CT-2 (detected in 5 cultures), CT-75 (detected in 2 cultures), and CT-5803 (detected in 1 culture). All sequence types (except CT-5803) were detected in Moscow in previous years. Using Internet database (http://pubmlst.org/neisseria) they were genetically characterized and compared with data on serogroup A meningococci circulated in Moscow before 2002., meningococci belonging to epidemically dangerous genetic subgroup III were not detected between characterized strains. Typed isolates were distributed between subgroups VI and X, which are typical for the area under surveillance. Genetic changes in Moscow population of Neisseria meningitidis serogroup A, which manifested by shift of dominating genetic subgroup after 2002-2003, were analyzed.

[Methandrostenolone residues in the tissues and biological fluids of agricultural animals stimulated by the preparation]. / Ostatochnye kolichestva metandrostenolona v tkaniakh i biologicheskikh zhidkostiakh sel'skokhoziaistvennykh zhivotnykh, stimulirovannykh preparatom.

Residual amounts of hormonal-active substances were isolated from the tissues of farm animals stimulated with metandrostenolone-3H. The process of isolation included extraction with a mixture of polar solvents, hydrolysis, adsorption and partition column chromatography, thin-layer and paper chromatography, with radio-isotope, fluorometric, spectrophotometric and densitometric methods employed for identification. Results subsequent to determination of residual amounts of the compound are reported.

[Influence of methandrostenolone on some aspects of protein metabolism in rats in the context of various protein rations]. / O vliianii metandrostenolona na nekotorye storony belkovogo obmena u krys na fone razlichnogo belkovogo pitaniia

Wistar male-rats receiving by mouth dianobol for 10 days and 9 months and fed on rations with different proportions of protein were investigated. Dianobol is shown to produce under definite conditions changes in the intensity of the protein synthesis in the muscle tissue and liver of the test animals and also to raise the activity of tryptophan-peroxidase and of alanine-amintransferase, the protein level in the ration being here of substantial importance. With its long-term administration to rats dianobol displays an androgenic effect on the gonads and the supernumerally genitals without exercising any stimulating action on the weight gain.

[Comparative evaluation of chemical methods for determining diethylstilbestrol in tissues of animal origin]. / Sravnitel'naia otsenka khimicheskikh metodov opredeleniia diétilstil'béstrola v tkaniakh zhivotnogo proiskhozhdeniia.

Three chemical methods for determination diethylstilbestrol (DES) in biological material (Jons and Deferage, 1953; Ponder, 1966; Official Method of Pharmacopoeia, USA, 1965) were compared. The most adequate method for determination of DES in animal tissue is the Official method of Pharmacopoeia based on a specific reaction. The method yields reproducible results. Average recovery of pure DES added to biological material was 78%. The method by Jons and Deferage though providing recovery of 86% of the added DES is based on nonspecific reaction with SbCl5, which resulted in too high results. Method of Pharmacopoeia (USA) was used to determine residual amounts of DES in tissues of a rabbit, implanted with 0.1 mg DES per 1 kg weight. One month after implantation, trace amounts of DES were detected only in muscle tissues. Two and three months after implantation, the residual amounts of DES in the rabbit tissues were not detected.