[Prevalence of arterial hypertension risk factors in open populations of Tiumen City, among individuals with stable basic social characteristics].

Studies of psychoemotional and hemodynamic parameters in active able-bodied age individuals with stable basic social characteristics, in emotionally stressed state, defined a group of males and females with first diagnosed arterial hypertension. The data obtained show leading role of prolonged emotional stress as an independent risk factor for arterial hypertension in special population groups of both sexes.

[The use of polymerase chain reaction for detection of tetracycline resistance urogenital mycoplasma ]. / Primenenie polimeraznoi tsepnoi reaktsii dlia vyiavleniia teratsiklinustochivykh shtammov urogenital'nykh mikoplazm.

PCR was used for detecting tetracyclin resistance genes tet-M and tet-O in Mollicutes. The tet determinants were detected in U. urealyticum and M. hominis genomes in 60 and 57.14%, respectively. PCR was used to detect urogenital mycoplasma in patients with different urogenital infections. The rate of detection of U. urealyticum was 10.71-25.1% in males and 23.08-61.91% in females, for M. hominis these values were 6.25-11.45% and 3.85-22.99%, and for M. genitalium 6.25-29.64% and 4.76-30.0%, respectively. Hence, the detection rate of tet determinants in mycoplasma by PCR was high (7-60%) in all groups of patients.

[Detection of tetracycline- and erythromycin-resistant urogenital Mycoplasma strains using PCR]. / Vyiavlenie tetratsiklin- i éritromitsinustoichivykh shtammov urogenital'nykh mikoplasm s pomoshch'iu PTsR.

The use of PCR made it possible to obtain data on the occurrence of genital mycoplasms in subjects with different pathological states of their urogenital system. The detection rate of U.urealyticum was 10.7-25.1% in males and 23.0-61.9% in females. The detection rate of Mycoplasma hominis was 6.2-11.4% in males and 3.8-22.9% in females. The detection rate of M.genitalium was 6.2-29.6% in males and 4.7-30.0% in females. The study carried out by means of amplification test systems for the detection of genes controlling resistance to tetracycline (tet-M and tet-O) and to erythromycin (erm) demonstrated that U.urealyticum contained the tet-determinant in 60% of cases and M.hominis, in 57.14% of cases. As revealed with the use of PCR techniques, the occurrence of erythromycin-resistant Ureaplasma strains was not high and was equal to 2.1%.

[Selective inhibition of DNA amplification in nonadhesive cultures of Mycoplasma pneumoniae]. / Izbiratel'noe ingibirovanie amplifikatsii DNK u neadgezivnykh kul'tur Mycoplasma pneumoniae.

Inhibition of amplification of various genome regions of Mycoplasma pneumoniae was observed in the polymerase chain reaction, and was dependent on cultivation conditions. A protein stably associated with DNA is responsible for the inhibitory effect. It is assumed that when the protein selectively associates with separate DNA regions, it can inhibit genes encoding pathogenicity factors, thus promoting mycoplasma transformation into persistent variants.

[The use of an amplification test system for detecting persistent mycoplasmas]. / Primenenie amplifikatsionnoi test-sistemy dlia vyiavleniia persistiruiushchikh mikoplazm.

A DNA amplification test system for the detection of Mycoplasma fermentans in clinical specimens was developed. The system was used for the analysis of biological specimens obtained from experimentally infected animals. The infective agent could be detected during the whole period of observation (6 months). High sensitivity of the polymerase chain reaction made it possible to detect M.fermentans in much greater number of cases than with the use of serological techniques.

[The effect of ionizing radiation at superhigh doses on the processes of dopamine release and reuptake by nerve endings in different sections of the brain]. / Vliianie ioniziruiushchego izlucheniia v sverkhvysokikh dozakh na protsessy vysvobozhdeniia i obratnogo zakhvata dofamina nervnymi terminaliami razlichnykh otdelov golovnogo mozga.

Rats exposed to fast 24 MeV electrons (100 Gy) at the state of early transient incapacity (ETI) exhibited active release and reuptake of dopamine in nerve terminals of the striatum. No changes in the indices under study were found in rats exposed to 25 Gy radiation that did not cause the ETI development. The in vitro irradiation of the isolated synaptosomes (100 Gy) inhibited dopamine reuptake and increased the number of sites of 3H-spiperone binding to D2-receptors in a membrane fraction isolated from the striatum.