Ordered arrangement and rearrangement of chromosomes during spermatogenesis in two species of planarians (Plathelminthes).

The pattern of distribution of telomeric DNA (TTAGGG), 28S rDNA, and 5S rDNA has been studied using fluorescence in situ hybridization (FISH) and primed in situ labelling during spermatogenesis and sperm formation in the filiform spermatozoa of two species of planarians, Dendrocoelum lacteum and Polycelis tenuis (Turbellaria, Plathelminthes). In both species, the positions of FISH signals found with each probe sequence are constant from cell to cell in the nuclei of mature sperm. Chromosome regions containing 5S and 28S rDNA genes are gathered in distinct bundles of spiral form. In early spermatids with roundish nuclei, the sites of a given sequence on different chromosomes remain separate. Centromeres (marked by 5S rDNA) gather into a single cluster in the central region of the slightly elongated sperm nucleus. During spermatid maturation, this cluster migrates to the distal pole of the nucleus. In Polycelis, telomeric sites gather into three distinct clusters at both ends and in the middle of the moderately elongated nucleus. These clusters retain their relative positions as the spermatid matures. All the chromosome ends bearing 28S rDNA gather only into the proximal cluster. Our data suggest that structures in the nucleus selectively recognise chromosome regions containing specific DNA sequences, which helps these regions to find their regular places in the mature sperm nucleus and causes clustering of the sites of these sequences located on different chromosomes. This hypothesis is supported by observations on elongated sperm of other animals in which a correlation exists between ordered arrangement of chromosomes in the mature sperm nucleus and clustering of sites of the same sequence from different chromosomes during spermiogenesis.

Unordered arrangement of chromosomes in the nuclei of chicken spermatozoa.

The arrangement of chromosomes in the elongated sperm nuclei of chicken was studied using fluorescence in situ hybridization with probes specific for telomeres of all chromosomes, a microchromosome, the long arm of chromosome 6, the large heterochromatic block on the Z-chromosome, and the same heterochromatic block plus subtelomeric sites on macrochromosomes 1-4. The positions of all probes vary from one sperm to another. No order in chromosome arrangement is apparent. It is suggested that large chromosome size and small chromosome number correlate with constant positions of chromosomes and vice versa. Based on the known quantity of repetitive units of the repeat on the Z-chromosome, the degree of compaction of chromatin in the chicken sperm nucleus is estimated as ca 0.7 Mb/ microm. As judged from the length of the heterochromatic region of the Z-chromosome at the lampbrush stage, the total length of the Z-chromosome in mature sperm is 2.5-4 times that of the sperm nucleus.

Transcription of lampbrush chromosomes of a centromerically localized highly repeated DNA in pigeon (Columba) relates to sequence arrangement.

A highly repetitive, centromerically localized DNA sequence (PR1) has been isolated from the genomic DNA of two species of pigeon (Columba livia and C. palumbus). PR1 is approximately 900 bp long. It includes a sequence that is similar to the CENP-B box of mammals. It represents about 5% of the genome in C. livia and 2% in C. palumbus. In both species, tandem arrays of PR1 form part of larger repeating units. The organization of PR1 repeats and the larger repeating units is strikingly different in the two species. The large repeating units in C. livia include long (at least 14 units) tandem arrays of PR1 interspersed with relatively short intervening sequences. The large repeats of C. palumbus have much shorter (4 units or fewer) PR1 arrays interspersed with longer sections of non-PR1 DNA. PR1 is transcribed on short lampbrush loops in the centromeric regions of all lampbrush bivalents of C. palumbus. In C. livia, it is not transcribed at any of the major pericentromeric sites at which it is known to be present, although it is transcribed at one minor centromeric site on chromosome 2. It is proposed that transcription of the noncoding PR1 sequence on lampbrush chromosomes of pigeons relates to its genomic organization. The proposal is discussed with regard to the 'read-through' hypothesis for transcription on lampbrush loops.

[Chiasma distribution in the lampbrush chromosomes of the chicken Gallus gallus domesticus: hot spots of recombination and their possible role in proper dysjunction of homologous chromosomes at the first meiotic division]. / Raspredelenie khiazm po khromosomam--lampovym shchetkam kuritsy Gallus gallus domesticus: goriachie tochki rekombinatsii i ikh vozmozhnoe znachenie dlia pravil'nogo raskhozhdeniia gomologichnykh khromosom v pervom meioticheskom delenii.

Chiasma distribution in the lambrush chromosomes of the chicken Gallus gallus domesticus was studied. The data of the authors show that the general pattern of chiasmata in the interstitional region of chromosomes corresponds to the Poisson distribution. However, in the telomeric and subtelomeric regions of all chicken macrochromosomes one can see chiasma as a rule. In the half of 140 microchromosomes from 24 different oocytes, there are also the telomeric chiasmata. On the basis of this observation, it may be predicted that there are hot spots of recombination near or into the telomeric GC-rich heterochromatic bands of chicken chromosomes. We suggest that these hot spots of recombination near the telomeres are a necessary facility for not only macrochromosomes but all microchromosomes as well to have at least one chiasma. The constant presence of at least one chiasma in a bivalent in needed for correct disjunction of homologous chromosomes at the first meiotic division.

[The lampbrush chromosomes of the chicken. Cytological maps of the macrobivalents]. / Khromosomy-lampovye shchetki kuritsy. Tsitologicheskie karty makrobivalentov.

The lampbrush chromosomes (LBC) were prepared from growing oocytes 0.75-1.50 mm in diameter. A map of 6 autosomes and the ZW sex bivalents is presented. Several types of landmarks were noticed: lumpy loops (LL), telomeric bow-like loops (TBL), some large loops in interstitial regions (marker loops--ML). Supposedly, the centromeres of LBC in the chicken are at one of the axial bars bearing no loops. The landmarks PBL and DBL mark the proximal and distal boundaries of bars. LBC-A (probably, chromosome 1 of the chicken karyotype) is about 185 microns. There are 7.3 +/- 0.2 chiasmata. Chiasmata are distributed at quasi-random. In LBC-A one chiasma is localized in a telomere, as a rule. Coordinates of 13 of the 14 different landmarks in LBC-A have been estimated. LBC-B (probably, chromosome 2) is about 151 microns, there are 5.50 +/- 0.23 chiasmata. The LBC-B may be identified by LL-21 and LL-22. LBC-C (probably, chromosome 3) is 128 microns; there are 4.70 +/- 0.18 chiasmata. The chromosome can be identified by characteristic loops LL-31, an unlooped chromomere bar near the telomere (T-32), a characteristic distribution of normal loops along LBC-C: about one half of this LBC bears large loops, and the other one--small loops. LBC-D (chromosome 4?) is 107 microns; there are 3.80 +/- 0.31 chiasmata. Double-loop bridges appear frequently near ML-41. LBC-E (chromosome 5?) is about 72 microns with 2.50 +/- 0.28 chiasmata. There are characteristic TBL loops with abundant RNP material thus being like LL-loops. LBC-F (chromosome 8?) is about 36.5 microns; there are 2 chiasmata. This LBC can be identified by giant telomeric loops GML-F1 and by unlooped bar in the middle of LBC.