Influence of protein nativity on the stability of bovine serum albumin coated microbubbles.

Protein-coated microbubbles have become one of the emerging platforms in biomedical research as theranostic agents. In recent years, microbubbles have been extensively used as ultrasound contrast agents and carriers of molecular cargoes, pertaining to which several studies have focused on tuning the properties of these bubbles to achieve a higher degree of biocompatibility and extended stability. Synthesis of microbubbles has so far been traditionally carried out with pre-heated proteins like bovine serum albumin (BSA) as shell coatings, owing to the ease in making BSA crosslinked structures through disulfide bridge formation. We, however, have performed experiments to demonstrate that air core microbubbles formed with native BSA are more stable compared with those formed using denatured BSA. The experimental observations have been supported with analytical modeling and computational studies, which offer insights into the effect of BSA conformation in stabilizing the microbubbles shells and prolonging their lifetimes.

Rapid synthesis of Pt(0) motors-microscrolls on a nickel surface via H2PtCl6-induced galvanic replacement reaction.

In this study, Pt(0) microscrolls are synthesized on polished Ni via galvanic replacement reaction (GRR). Employing in situ optical microscopy, the dynamic motion of the catalytic microscrolls as micromotors in H2O2 solutions is revealed. This method offers a rapid fabrication of scrolls from diverse noble metals and alloys.

Membraneless Hydrogen Peroxide Fuel Cells as a Promising Clean Energy Source.

In an in-depth investigation of membraneless hydrogen peroxide-based fuel cells (H2O2 FCs), hydrogen peroxide (H2O2), a carbon-neutral compound, is demonstrated to undergo electrochemical decomposition to produce H2O, O2, and electrical energy. The unique redox properties of H2O2 position it as a viable candidate for sustainable energy applications. The proposed membraneless design addresses the limitations of conventional fuel cells, including fabrication complexities and design challenges. A novel three-dimensional electrode, synthesized via electroplating techniques, is introduced. Constructed from Au-electroplated carbon fiber cloth combined with Ni-foam, this electrode showcases enhanced electrochemical reaction kinetics, leading to an increased power density for H2O2 FCs. The performance of fuel cells is intricately linked to the pH levels of the electrolyte solution. Beyond FC applications, such electrodes hold potential in portable energy systems and as high surface area catalysts. This study emphasizes the significance of electrode engineering in optimizing the potential of H2O2 as an environmentally friendly energy source.

Bridging the gap: harnessing liquid nanomachine know-how for tackling harmful airborne particulates.

The emergence of "nanomotors", "nanomachines", and "nanorobotics" has transformed dynamic nanoparticle research, driving a transition from passive to active and intelligent nanoscale systems. This review examines two critical fields: the investigation of airborne particles, significant contributors to air pollution, and the rapidly emerging domain of catalytic and field-controlled nano- and micromotors. We examine the basic concepts of nano- and micromachines in motion and envision their possible use in a gaseous medium to trap and neutralize hazardous particulates. While past studies described the application of nanotechnology and nanomotors in various scenarios, airborne nano/micromachine motion and their control have yet to be thoroughly explored. This review intends to promote multidisciplinary research on nanomachines' propulsion and task-oriented applications, highlighting their relevance in obtaining a cleaner atmospheric environment, a critical component to consider for human health.

Albumin microbubbles conjugated with zinc and aluminum phthalocyanine dyes for enhanced photodynamic activity.

Gas-liquid interfaces are reaching a particular interest in biomedicine. Microbubbles, ultrasound contrast agents of clinical routine, gained increasing attention as theranostic platforms due to the preserved acoustic response, drug conjugation capabilities, and applicability in biological barrier opening. A combination of microbubbles and photodynamic therapy agents can enhance the photodynamic effect, yet the evaluation of agent conjugation on microbubble stabilization and photodynamic effect is needed. Hence, two commercially available phthalocyanine photosensitizers - Holosens® (ZnPc) and Photosens® (AlPc) - were coupled with bovine serum albumin before microbubble synthesis. We demonstrated an albumin: phthalocyanine ratio of 1:1 and covalent attachment for ZnPc, a ratio of 1:3 with electrostatic binding for AlPc. Submicron-sized microbubbles (air- and SF6- filled) had a diameter of 0.8 µm. Albumin-phthalocyanine conjugates increased the microbubble concentration and shelf-life stability compared to plain ones. We hypothesized that phthalocyanine fluorescence lifetime values decreased after conjugation with microbubbles due to narrow distance between conjugates in the shell. Agents based on AlPc demonstrated higher photodynamic activity than agents based on ZnPc, and microbubbles preserved acoustic stability in human blood plasma. The biodistribution of AlPc-conjugated microbubbles was evaluated. We conclude that our microbubble platforms demonstrate greater photodynamic activity and prolonged stability for further applications in photodynamic therapy.

Air-Filled Microbubbles Based on Albumin Functionalized with Gold Nanocages and Zinc Phthalocyanine for Multimodal Imaging.

Microbubbles are intravascular contrast agents clinically used in diagnostic sonography, echocardiography, and radiology imaging applications. However, up to date, the idea of creating microbubbles with multiple functionalities (e.g., multimodal imaging, photodynamic therapy) remained a challenge. One possible solution is the modification of bubble shells by introducing specific compounds responsible for such functions. In the present work, air-core microbubbles with the shell consisting of bovine serum albumin, albumin-coated gold nanocages, and zinc phthalocyanine were prepared using the sonication method. Various physicochemical parameters such as stability over time, size, and concentration were investigated to prove the potential use of these microbubbles as contrast agents. This work shows that hybrid microbubbles have all the necessary properties for multimodal imaging (ultrasound, raster-scanning microscopy, and fluorescence tomography), which demonstrate superior characteristics for potential theranostic and related biomedical applications.

Oxygen Generation Using Catalytic Nano/Micromotors.

Gaseous oxygen plays a vital role in driving the metabolism of living organisms and has multiple agricultural, medical, and technological applications. Different methods have been discovered to produce oxygen, including plants, oxygen concentrators and catalytic reactions. However, many such approaches are relatively expensive, involve challenges, complexities in post-production processes or generate undesired reaction products. Catalytic oxygen generation using hydrogen peroxide is one of the simplest and cleanest methods to produce oxygen in the required quantities. Chemically powered micro/nanomotors, capable of self-propulsion in liquid media, offer convenient and economic platforms for on-the-fly generation of gaseous oxygen on demand. Micromotors have opened up opportunities for controlled oxygen generation and transport under complex conditions, critical medical diagnostics and therapy. Mobile oxygen micro-carriers help better understand the energy transduction efficiencies of micro/nanoscopic active matter by careful selection of catalytic materials, fuel compositions and concentrations, catalyst surface curvatures and catalytic particle size, which opens avenues for controllable oxygen release on the level of a single catalytic microreactor. This review discusses various micro/nanomotor systems capable of functioning as mobile oxygen generators while highlighting their features, efficiencies and application potentials in different fields.

CT-Based COVID-19 triage: Deep multitask learning improves joint identification and severity quantification.

The current COVID-19 pandemic overloads healthcare systems, including radiology departments. Though several deep learning approaches were developed to assist in CT analysis, nobody considered study triage directly as a computer science problem. We describe two basic setups: Identification of COVID-19 to prioritize studies of potentially infected patients to isolate them as early as possible; Severity quantification to highlight patients with severe COVID-19, thus direct them to a hospital or provide emergency medical care. We formalize these tasks as binary classification and estimation of affected lung percentage. Though similar problems were well-studied separately, we show that existing methods could provide reasonable quality only for one of these setups. We employ a multitask approach to consolidate both triage approaches and propose a convolutional neural network to leverage all available labels within a single model. In contrast with the related multitask approaches, we show the benefit from applying the classification layers to the most spatially detailed feature map at the upper part of U-Net instead of the less detailed latent representation at the bottom. We train our model on approximately 1500 publicly available CT studies and test it on the holdout dataset that consists of 123 chest CT studies of patients drawn from the same healthcare system, specifically 32 COVID-19 and 30 bacterial pneumonia cases, 30 cases with cancerous nodules, and 31 healthy controls. The proposed multitask model outperforms the other approaches and achieves ROC AUC scores of 0.87±0.01 vs. bacterial pneumonia, 0.93±0.01 vs. cancerous nodules, and 0.97±0.01 vs. healthy controls in Identification of COVID-19, and achieves 0.97±0.01 Spearman Correlation in Severity quantification. We have released our code and shared the annotated lesions masks for 32 CT images of patients with COVID-19 from the test dataset.

Micro-Bio-Chemo-Mechanical-Systems: Micromotors, Microfluidics, and Nanozymes for Biomedical Applications.

Wireless nano-/micromotors powered by chemical reactions and/or external fields generate motive forces, perform tasks, and significantly extend short-range dynamic responses of passive biomedical microcarriers. However, before micromotors can be translated into clinical use, several major problems, including the biocompatibility of materials, the toxicity of chemical fuels, and deep tissue imaging methods, must be solved. Nanomaterials with enzyme-like characteristics (e.g., catalase, oxidase, peroxidase, superoxide dismutase), that is, nanozymes, can significantly expand the scope of micromotors' chemical fuels. A convergence of nanozymes, micromotors, and microfluidics can lead to a paradigm shift in the fabrication of multifunctional micromotors in reasonable quantities, encapsulation of desired subsystems, and engineering of FDA-approved core-shell structures with tuneable biological, physical, chemical, and mechanical properties. Microfluidic methods are used to prepare stable bubbles/microbubbles and capsules integrating ultrasound, optoacoustic, fluorescent, and magnetic resonance imaging modalities. The aim here is to discuss an interdisciplinary approach of three independent emerging topics: micromotors, nanozymes, and microfluidics to creatively: 1) embrace new ideas, 2) think across boundaries, and 3) solve problems whose solutions are beyond the scope of a single discipline toward the development of micro-bio-chemo-mechanical-systems for diverse bioapplications.

Air-Filled Bubbles Stabilized by Gold Nanoparticle/Photodynamic Dye Hybrid Structures for Theranostics.

Microbubbles have already reached clinical practice as ultrasound contrast agents for angiography. However, modification of the bubbles' shell is needed to produce probes for ultrasound and multimodal (fluorescence/photoacoustic) imaging methods in combination with theranostics (diagnostics and therapeutics). In the present work, hybrid structures based on microbubbles with an air core and a shell composed of bovine serum albumin, albumin-coated gold nanoparticles, and clinically available photodynamic dyes (zinc phthalocyanine, indocyanine green) were shown to achieve multimodal imaging for potential applications in photodynamic therapy. Microbubbles with an average size of 1.5 ± 0.3 µm and concentration up to 1.2 × 109 microbubbles/mL were obtained and characterized. The introduction of the dye into the system reduced the solution's surface tension, leading to an increase in the concentration and stability of bubbles. The combination of gold nanoparticles and photodynamic dyes' influence on the fluorescent signal and probes' stability is described. The potential use of the obtained probes in biomedical applications was evaluated using fluorescence tomography, raster-scanning optoacoustic microscopy and ultrasound response measurements using a medical ultrasound device at the frequency of 33 MHz. The results demonstrate the impact of microbubbles' stabilization using gold nanoparticle/photodynamic dye hybrid structures to achieve probe applications in theranostics.

Requirement and Development of Hydrogel Micromotors towards Biomedical Applications.

With controllable size, biocompatibility, porosity, injectability, responsivity, diffusion time, reaction, separation, permeation, and release of molecular species, hydrogel microparticles achieve multiple advantages over bulk hydrogels for specific biomedical procedures. Moreover, so far studies mostly concentrate on local responses of hydrogels to chemical and/or external stimuli, which significantly limit the scope of their applications. Tetherless micromotors are autonomous microdevices capable of converting local chemical energy or the energy of external fields into motive forces for self-propelled or externally powered/controlled motion. If hydrogels can be integrated with micromotors, their applicability can be significantly extended and can lead to fully controllable responsive chemomechanical biomicromachines. However, to achieve these challenging goals, biocompatibility, biodegradability, and motive mechanisms of hydrogel micromotors need to be simultaneously integrated. This review summarizes recent achievements in the field of micromotors and hydrogels and proposes next steps required for the development of hydrogel micromotors, which become increasingly important for in vivo and in vitro bioapplications.

Parameters Optimization of Catalytic Tubular Nanomembrane-Based Oxygen Microbubble Generator.

A controllable generation of oxygen gas during the decomposition of hydrogen peroxide by the microreactors made of tubular catalytic nanomembranes has recently attracted considerable attention. Catalytic microtubes play simultaneous roles of the oxygen bubble producing microreactors and oxygen bubble-driven micropumps. An autonomous pumping of peroxide fuel takes place through the microtubes by the recoiling microbubbles. Due to optimal reaction-diffusion processes, gas supersaturation, leading to favorable bubble nucleation conditions, strain-engineered catalytic microtubes with longer length produce oxygen microbubbles at concentrations of hydrogen peroxide in approximately ×1000 lower in comparison to shorter tubes. Dynamic regimes of tubular nanomembrane-based oxygen microbubble generators reveal that this depends on microtubes' aspect ratio, hydrogen peroxide fuel concentration and fuel compositions. Different dynamic regimes exist, which produce specific bubble frequencies, bubble size and various amounts of oxygen. In this study, the rolled-up Ti/Cr/Pd microtubes integrated on silicon substrate are used to study oxygen evolution in different concentrations of hydrogen peroxide and surfactants. Addition of Sodium dodecyl sulfate (SDS) surfactants leads to a decrease of bubble diameter and an increase of frequencies of bubble recoil. Moreover, an increase of temperature (from 10 to 35 °C) leads to higher frequencies of oxygen bubbles and larger total volumes of produced oxygen.

Catalytic/magnetic assemblies of rolled-up tubular nanomembrane-based micromotors.

Nano/-micromotors self-assembling into static and dynamic clusters are of considerable promise to study smart, interactive, responsive, and adaptive nano/-micromaterials that can mimic spatio-temporal patterns, swarming, and collective behaviors widely observed in nature. Previously, the dynamic self-assembly of bubble-propelled catalytic micromotors initiated by capillary forces has been reported. This manuscript shows novel self-assembly modes of magnetic/catalytic Ti/FeNi/Pt tubular micromotors. When chemical fuel (hydrogen peroxide) is added it is decomposed on contact with Pt catalyst into oxygen and water. Here, the non-bubbling motion and autonomous assembly of catalytic/magnetic nanomembranes, i.e. without nucleation/generation of oxygen bubbles, are shown. Moreover, magnetic Ti/FeNi/Pt micromotors are spun using an external magnetic field and they form dynamic clusters balanced by attractive magnetic and repulsive hydrodynamic interactions. Micromotors form dynamic clusters, undergo precession and rapidly propagate through the solution.

Oxygen Microbubble Generator Enabled by Tunable Catalytic Microtubes.

Rolled-up catalytic Ti/Cr/Pt microtubes, consisting of inorganic nanomembranes integrated on-chip, are used to generate oxygen microbubbles in solutions of hydrogen peroxide. Oxygen bubble parameters (frequency, radius and volumetric flow rate) are optimized at different concentrations of hydrogen peroxide and common dish soap. Increasing the aspect ratio of the microtube (e.g., tube length/diameter) leads to the formation of smaller bubbles, but at higher frequencies. Longer tubes produce less total oxygen volume in comparison to shorter tubes. We attribute this observation to the specific dynamic behaviours of bubbles in tubes.

Hydrogel micromotors with catalyst-containing liquid core and shell.

Methacrylic anhydride-derived hydrogel microcapsules have unique properties, including reversibly tunable permeation, purification, and separation of dissolved molecular species. Endowing these dynamic encapsulant systems with autonomous motion will significantly enhance their efficiency and applicability. Here, hydrogel micromotors are realized using complex water-in-oil-in-water double emulsion drops and oil-in-water emulsion drops from glass capillary microfluidics and subsequent photopolymerization. Three hydrogel micromotor strategies are explored: microcapsules with thin shells and liquid cores with dispersed catalytic Pt nanoparticles, as well as water-cored microcapsules and homogeneous microparticles selectively coated with Ti/Pt catalytic layers. Autonomous motion of hydrogel particles and capsules is realized in hydrogen peroxide solutions, where generated oxygen microbubbles propel the dynamically responsive micromotors. The micromotors are balanced by weight, buoyancy, lateral capillary forces and show specific autonomous behaviours that significantly extend short range dynamic responses of hydrogels. Drop-based microfluidics represent a paradigm shift in the integration of multifunctional subsystems and high-throughput design of chemical micromachines in reasonable quantities towards their desired biomedical, environmental and flow/diffusion microreactor applications.

Light-controlled two-dimensional TiO2 plate micromotors.

In this work, UV light-controlled two-dimensional (2D) TiO2 plate micromotors are demonstrated for the first time. The 2D TiO2 micromotors are produced by the well-known anodic oxidation method in combination with a cracking and separation process. When the motor is placed in H2O2 aqueous solution under UV irradiation, oxygen bubbles are generated in the holes of the TiO2 membrane. The 2D micromotor thus moves upon O2 bubbles under its own weight. In contrast to bubble-propelled micromotors, which require an addition of surfactants to chemical fuels, the 2D micromotor is capable of moving in aqueous H2O2 solution without surfactants. Moreover, speed of the 2D TiO2 micromotor can be controlled by the intensity of the UV light. Such surfactant-free micromotors and their facile fabrication hold considerable promise for diverse practical applications in the biomedical and energy fields, for example, and in new materials.

Geometry Design, Principles and Assembly of Micromotors.

Discovery of bio-inspired, self-propelled and externally-powered nano-/micro-motors, rotors and engines (micromachines) is considered a potentially revolutionary paradigm in nanoscience. Nature knows how to combine different elements together in a fluidic state for intelligent design of nano-/micro-machines, which operate by pumping, stirring, and diffusion of their internal components. Taking inspirations from nature, scientists endeavor to develop the best materials, geometries, and conditions for self-propelled motion, and to better understand their mechanisms of motion and interactions. Today, microfluidic technology offers considerable advantages for the next generation of biomimetic particles, droplets and capsules. This review summarizes recent achievements in the field of nano-/micromotors, and methods of their external control and collective behaviors, which may stimulate new ideas for a broad range of applications.

Carbon dioxide bubble-propelled microengines in carbonated water and beverages.

We demonstrate a new type of gaseous fuel for rolled-up tubular Ti/Cr microengine powered by carbon dioxide microbubbles in carbonated water and brewed beverages. Existence of microbubble pockets is revealed using on-chip integrated transparent microtubes. Vertical position, angle of rotation and buoyancy of ferromagnetic Ti/Fe/Cr microengines are controlled using an external magnetic field.

Local-Curvature-Controlled Non-Epitaxial Growth of Hierarchical Nanostructures.

Site-selective growth on non-spherical seeds provides an indispensable route to hierarchical complex nanostructures that are interesting for diverse applications. However, this has only been achieved through epitaxial growth, which is restricted to crystalline materials with similar crystal structures and physicochemical properties. A non-epitaxial growth strategy is reported for hierarchical nanostructures, where site-selective growth is controlled by the curvature of non-spherical seeds. This strategy is effective for site-selective growth of silica nanorods from non-spherical seeds of different shapes and materials, such as α-Fe2 O3 , NaYF4 , and ZnO. This growth strategy is not limited by the stringent requirements of epitaxy and is thus a versatile general method suitable for the preparation of hierarchical nanostructures with controlled morphologies and compositions to open up a verity of applications in self-assembly, nanorobotics, catalysis, electronics, and biotechnology.

Collective behaviour of self-propelled catalytic micromotors.

Biology widely employs catalytic reactions to power biomotors and cells. These dynamic entities can self-organize into swarms or self-assemble into functional micro- or nanostructures. Synthetic micro-/nanojet engines and nanomotors, driven by catalytic reactions, can move with high power and perform multiple tasks. Collective behavior of these microengines has recently been observed which includes swarming activities and the formation of multiconstituent entities. This feature article discusses recent developments, presents new discoveries on collective motion of self-propelled microjet engines and suggests next steps to undertake in the field of collective micromachines.