RESUMO
The Agr family genes, Ag1, Agr2, and Agr3, encode for the thioredoxin domain containing secreted proteins and are specific only for vertebrates. These proteins are attracting increasing attention due to their involvement in many physiological and pathological processes, including exocrine secretion, cancer, regeneration of the body appendages, and the early brain development. At the same time, the mode by which Agrs regulate intracellular processes are poorly understood. Despite that the receptor to Agr2, the membrane anchored protein Prod1, has been firstly discovered in Urodeles, and it has been shown to interact with Agr2 in the regenerating limb, no functional homologs of Prod1 were identified in other vertebrates. This raises the question of the mechanisms by which Agrs can regulate regeneration in other lower vertebrates. Recently, we have identified that Tfp4 (three-fingers Protein 4), the structural and functional homolog of Prod1 in Anurans, interacts with Agr2 in Xenopus laevis embryos. In the present work we show by several methods that the activity of Tfp4 is essential for the tadpole tail regeneration as well as for the early eye and forebrain development during embryogenesis. These data show for the first time the common molecular mechanism of regeneration regulation in amphibians by interaction of Prod1 and Agr2 proteins.
Assuntos
Arginase/metabolismo , Regulação da Expressão Gênica no Desenvolvimento/genética , Regeneração/fisiologia , Proteínas de Xenopus/metabolismo , Animais , Proteínas de Transporte/metabolismo , Desenvolvimento Embrionário , Extremidades/embriologia , Larva/genética , Larva/metabolismo , Organogênese , Ligação Proteica/fisiologia , Regeneração/genética , Tiorredoxinas/metabolismo , Proteínas de Xenopus/genética , Xenopus laevis/metabolismoRESUMO
The secreted protein Noggin1 is an embryonic inducer that can sequester TGFß cytokines of the BMP family with extremely high affinity. Owing to this function, ectopic Noggin1 can induce formation of the headless secondary body axis in Xenopus embryos. Here, we show that Noggin1 and its homolog Noggin2 can also bind, albeit less effectively, to ActivinB, Nodal/Xnrs and XWnt8, inactivation of which, together with BMP, is essential for the head induction. In support of this, we show that both Noggin proteins, if ectopically produced in sufficient concentrations in Xenopus embryo, can induce a secondary head, including the forebrain. During normal development, however, Noggin1 mRNA is translated in the presumptive forebrain with low efficiency, which provides the sufficient protein concentration for only its BMP-antagonizing function. By contrast, Noggin2, which is produced in cells of the anterior margin of the neural plate at a higher concentration, also protects the developing forebrain from inhibition by ActivinB and XWnt8 signaling. Thus, besides revealing of novel functions of Noggin proteins, our findings demonstrate that specification of the forebrain requires isolation of its cells from BMP, Activin/Nodal and Wnt signaling not only during gastrulation but also at post-gastrulation stages.
Assuntos
Ativinas/metabolismo , Proteínas de Transporte/metabolismo , Via de Sinalização Wnt , Animais , Proteínas Morfogenéticas Ósseas/metabolismo , Regulação da Expressão Gênica no Desenvolvimento , Mesoderma/metabolismo , Placa Neural/metabolismo , Prosencéfalo/crescimento & desenvolvimento , Prosencéfalo/metabolismo , Ligação Proteica , Proteínas Wnt/metabolismo , Xenopus/embriologia , Xenopus/metabolismo , Proteínas de Xenopus/metabolismoRESUMO
Proteins of the GFP (green fluorescent protein) family are widely used as passive reporters for live cell imaging. In the present study we used H2B (histone H2B)-tKR (tandem KillerRed) as an active tool to affect cell division with light. We demonstrated that H2B-tKR-expressing cells behave normally in the dark, but transiently cease proliferation following green-light illumination. Complete light-induced blockage of cell division for approx. 24 h was observed in cultured mammalian cells that were either transiently or stably transfected with H2B-tKR. Illuminated cells then returned to normal division rate. XRCC1 (X-ray cross complementing factor 1) showed immediate redistribution in the illuminated nuclei of H2B-tKR-expressing cells, indicating massive light-induced damage of genomic DNA. Notably, nondisjunction of chromosomes was observed for cells that were illuminated during metaphase. In transgenic Xenopus embryos expressing H2B-tKR under the control of tissue-specific promoters, we observed clear retardation of the development of these tissues in green-light-illuminated tadpoles. We believe that H2B-tKR represents a novel optogenetic tool, which can be used to study mitosis and meiosis progression per se, as well as to investigate the roles of specific cell populations in development, regeneration and carcinogenesis in vivo.
Assuntos
Divisão Celular/efeitos da radiação , Cromatina/metabolismo , Proteínas de Fluorescência Verde/metabolismo , Proteínas Luminescentes/metabolismo , Sondas Moleculares/metabolismo , Animais , Animais Geneticamente Modificados/embriologia , Animais Geneticamente Modificados/metabolismo , Núcleo Celular/metabolismo , Cromatina/efeitos da radiação , Dano ao DNA/efeitos da radiação , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/metabolismo , Embrião não Mamífero/citologia , Embrião não Mamífero/metabolismo , Embrião não Mamífero/efeitos da radiação , Proteínas de Fluorescência Verde/genética , Células HeLa , Histonas/genética , Histonas/metabolismo , Humanos , Luz , Proteínas Luminescentes/genética , Sondas Moleculares/genética , Neoplasias/metabolismo , Neoplasias/patologia , Neoplasias/ultraestrutura , Transporte Proteico/efeitos da radiação , RNA Mensageiro/metabolismo , Proteínas Recombinantes de Fusão/metabolismo , Proteína 1 Complementadora Cruzada de Reparo de Raio-X , Xenopus laevisRESUMO
A vast colour palette of monomeric fluorescent proteins has been developed to investigate protein localization, motility and interactions. However, low brightness has remained a problem in far-red variants, which hampers multicolour labelling and whole-body imaging techniques. In the present paper, we report mKate2, a monomeric far-red fluorescent protein that is almost 3-fold brighter than the previously reported mKate and is 10-fold brighter than mPlum. The high-brightness, far-red emission spectrum, excellent pH resistance and photostability, coupled with low toxicity demonstrated in transgenic Xenopus laevis embryos, make mKate2 a superior fluorescent tag for imaging in living tissues. We also report tdKatushka2, a tandem far-red tag that performs well in fusions, provides 4-fold brighter near-IR fluorescence compared with mRaspberry or mCherry, and is 20-fold brighter than mPlum. Together, monomeric mKate2 and pseudo-monomeric tdKatushka2 represent the next generation of extra-bright far-red fluorescent probes offering novel possibilities for fluorescent imaging of proteins in living cells and animals.
Assuntos
Corantes Fluorescentes , Proteínas Luminescentes , Sequência de Aminoácidos , Animais , Linhagem Celular , Diagnóstico por Imagem/métodos , Embrião não Mamífero , Fluorescência , Corantes Fluorescentes/química , Células HeLa , Humanos , Proteínas Luminescentes/química , Proteínas Luminescentes/genética , Dados de Sequência Molecular , Alinhamento de Sequência , Xenopus laevis , Proteína Vermelha FluorescenteRESUMO
For deep imaging of animal tissues, the optical window favorable for light penetration is in near-infrared wavelengths, which requires proteins with emission spectra in the far-red wavelengths. Here we report a far-red fluorescent protein, named Katushka, which is seven- to tenfold brighter compared to the spectrally close HcRed or mPlum, and is characterized by fast maturation as well as a high pH-stability and photostability. These unique characteristics make Katushka the protein of choice for visualization in living tissues. We demonstrate superiority of Katushka for whole-body imaging by direct comparison with other red and far-red fluorescent proteins. We also describe a monomeric version of Katushka, named mKate, which is characterized by high brightness and photostability, and should be an excellent fluorescent label for protein tagging in the far-red part of the spectrum.
Assuntos
Biotecnologia/métodos , Transferência Ressonante de Energia de Fluorescência/métodos , Corantes Fluorescentes , Proteínas Luminescentes , Microscopia Confocal/métodos , Microscopia de Fluorescência/métodos , Animais , Biotecnologia/instrumentação , Embrião não Mamífero/citologia , Embrião não Mamífero/metabolismo , Transferência Ressonante de Energia de Fluorescência/instrumentação , Corantes Fluorescentes/análise , Corantes Fluorescentes/química , Células HeLa , Humanos , Proteínas Luminescentes/análise , Proteínas Luminescentes/química , Proteínas Luminescentes/genética , Microscopia Confocal/instrumentação , Microscopia de Fluorescência/instrumentação , Transgenes , Xenopus laevis , Proteína Vermelha FluorescenteRESUMO
Early development of the rostral forebrain (RF) in vertebrates is accompanied by the inhibition of two homeobox regulators, Otx2 and Pax6 in the rostral sector of the anterior neural plate, further giving rise to the RF. However, the precise molecular mechanism and meaning of this inhibition is still obscure. We now demonstrate that the activity of the Anf homeodomain protein is necessary and sufficient for the anterior inhibition of Otx2 and Pax6. Specifically, we show that knockdown of the Xenopus laevis Anf, Xanf, by antisense morpholino oligonucleotides results in the anterior expansion of Otx2 and Pax6 expression into the presumptive RF territory. Furthermore, by overexpressing hormone-inducible activator- and repressor-fused variants of Xanf in the absence of protein synthesis, we present evidence that Xanf can directly downregulate Otx2 and Pax6 but not the more rostrally expressed Bf1, Bf2, Fgf8 and Nkx2.4. These results explain how the inhibitory activity of Xanf can discriminate RF regulators in favor of posterior forebrain ones. Assuming that the Anf type of homeobox is specific for vertebrates, our data suggest that the emergence of Anf in evolution could be a critical event for RF development in vertebrates through the elimination of homologues of modern posterior forebrain regulators from the rostral sector of the anterior neural plate.