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1.
Biotechnol Prog ; 40(1): e3400, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-37964726

RESUMO

In the production of biological therapeutics such as monoclonal antibodies (mAbs), ultrafiltration and diafiltration (UF/DF) are widely regarded as effective downstream processing steps capable of removing process equipment related leachables (PERLs) introduced upstream of the UF/DF step. However, clearance data available in the literature are limited to species with low partition coefficients (log P) such as buffer ions, hydrophilic organic compounds, and some metal ions. Additional data for a wide range of PERLs including hydrophobic compounds and elemental impurities are needed to establish meaningful, comprehensive safety risk assessments. Herein, we report the results from studies investigating the clearance of seven different organic PERLs representing a wide range of characteristics (i.e., log P (-0.3 to 18)), and four model elements with different chemical properties spiked into a mAb formulation at 10 ppm and analyzed during clearance using gas chromatography-mass spectrometry (GC-MS), liquid chromatography-photodiode-array-mass spectrometry (LC-PDA-MS), and inductively coupled plasma mass spectrometry (ICP-MS). The clearance data showed ideal clearance and sieving of spiked organic PERLs with log P < 4, partial clearance of PERLs with 4 < log P < 9, and poor clearance of highly hydrophobic PERLs (log P > 9) after nine diafiltration volumes (DVs). Supplemental clearance studies on seven additional PERLs present at much lower concentration levels (0.1-1.5 ppm) in the mAb formulation upstream of UF/DF and three PERLs associated with the tangential flow filtration (TFF) equipment also demonstrated the similar correlations between log P and % clearance. For model elements, the findings suggest that UF/DF in general provides ideal clearance for elements. Evidence showed that the UF/DF process does not only help mitigate leachables risk from PERLs introduced upstream of UF/DF, but also from the TFF operation itself as all three TFF-related PERLs were effectively cleared. Overall, the UF/DF clearance presented in this work demonstrated whereas highly hydrophobic PERLs and elements that exist as charged species, particularly transition metal ions, may not be as effectively cleared and thus warrant further risk assessment; hydrophilic and some hydrophobic PERLs (log P < 4) are indeed well-cleared and thus present a lower overall safety risk.


Assuntos
Filtração , Ultrafiltração , Ultrafiltração/métodos , Filtração/métodos , Compostos Orgânicos , Íons
2.
J Am Chem Soc ; 138(48): 15682-15689, 2016 12 07.
Artigo em Inglês | MEDLINE | ID: mdl-27934019

RESUMO

The rational and predictable enhancement of protein stability is an important goal in protein design. Most efforts target the folded state, however stability is the free energy difference between the folded and unfolded states thus both are suitable targets. Strategies directed at the unfolded state usually seek to decrease chain entropy by introducing cross-links or by replacing glycines. Cross-linking has led to mixed results. Replacement of glycine with an l-amino acid, while reducing the entropy of the unfolded state, can introduce unfavorable steric interactions in the folded state, since glycine is often found in conformations that require a positive φ angle such as helical C-capping motifs or type I' and II″ ß-turns. l-Amino acids are strongly disfavored in these conformations, but d-amino acids are not. However, there are few reported examples and conflicting results have been obtained when glycines are replaced with d-Ala. We critically examine the effect of Gly-to-d-Ala substitutions on protein stability using experimental approaches together with molecular dynamics simulations and free energy calculations. The data, together with a survey of high resolution structures, show that the vast majority of proteins can be stabilized by substitution of C-capping glycines with d-Ala. Sites suitable for substitutions can be identified via sequence alignment with a high degree of success. Steric clashes in the native state due to the new side chain are rarely observed, but are likely responsible for the destabilizing or null effect observed for the small subset of Gly-to-d-Ala substitutions which are not stabilizing. Changes in backbone solvation play less of a role. Favorable candidates for d-Ala substitution can be identified using a rapid algorithm based on molecular mechanics.


Assuntos
Proteínas de Bactérias/química , Proteínas de Homeodomínio/química , Proteínas dos Microfilamentos/química , Simulação de Dinâmica Molecular , Desdobramento de Proteína , Albumina Sérica/química , Termodinâmica , Fatores de Transcrição/química , Alanina/química , Algoritmos , Animais , Galinhas , Produtos Finais de Glicação Avançada , Glicina/química , Modelos Moleculares , Estabilidade Proteica , Albumina Sérica Glicada
3.
PLoS One ; 11(1): e0147991, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26808496

RESUMO

Hepatitis C virus (HCV) poses a global threat to public health. HCV envelop protein E2 is the major component on the virus envelope, which plays an important role in virus entry and morphogenesis. Here, for the first time, we affinity purified E2 complex formed in HCV-infected human hepatoma cells and conducted comparative mass spectrometric analyses. 85 cellular proteins and three viral proteins were successfully identified in three independent trials, among which alphafetoprotein (AFP), UDP-glucose: glycoprotein glucosyltransferase 1 (UGT1) and HCV NS4B were further validated as novel E2 binding partners. Subsequent functional characterization demonstrated that gene silencing of UGT1 in human hepatoma cell line Huh7.5.1 markedly decreased the production of infectious HCV, indicating a regulatory role of UGT1 in viral lifecycle. Domain mapping experiments showed that HCV E2-NS4B interaction requires the transmembrane domains of the two proteins. Altogether, our proteomics study has uncovered key viral and cellular factors that interact with E2 and provided new insights into our understanding of HCV infection.


Assuntos
Hepacivirus/fisiologia , Interações Hospedeiro-Patógeno , Fígado/virologia , Proteômica , Glucuronosiltransferase/metabolismo , Células HEK293 , Humanos , Fígado/metabolismo , Espectrometria de Massas em Tandem , Proteínas não Estruturais Virais/metabolismo , alfa-Fetoproteínas/metabolismo
4.
Chem Biol Drug Des ; 86(6): 1433-7, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26109481

RESUMO

Hemopressin is a naturally occurring and therapeutically relevant peptide with applications in hypertension, pain, addiction, and obesity. We had previously demonstrated that hemopressin converts into amyloid-like fibrils under aqueous conditions. However, the amino acid residues that modulate the aggregation propensity of hemopressin were not identified. In this study, we designed and synthesized 25 different analogs of hemopressin and analyzed their aggregation properties using the principle of dynamic light scattering. As a result, we were able to identify four conservative changes in the peptide sequence (Val(2) →DVal(2), Asn(3) →Gln(3) Leu(7) →Npg(7) and C-OH→C-NH2) that minimize aggregation propensity of hemopressin. The results indicate that hemopressin aggregation is cooperative in nature and involves contribution from multiple amino acids within the peptide chain. The analogs and the corresponding aggregation propensity data reported in this study would be useful for researchers investigating therapeutic properties of hemopressin, which have been hampered due to the tendency of hemopressin to aggregate in aqueous solutions.


Assuntos
Hemoglobinas/química , Fragmentos de Peptídeos/química , Sequência de Aminoácidos , Substituição de Aminoácidos , Animais , Desenho de Fármacos , Difusão Dinâmica da Luz , Hemoglobinas/síntese química , Hemoglobinas/farmacologia , Humanos , Hidrodinâmica , Camundongos , Dados de Sequência Molecular , Tamanho da Partícula , Fragmentos de Peptídeos/síntese química , Fragmentos de Peptídeos/farmacologia , Agregados Proteicos
5.
PLoS One ; 9(10): e110980, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25333937

RESUMO

Vaginal microbicides hold great promise for the prevention of viral diseases like HIV, but the failure of several microbicide candidates in clinical trials has raised important questions regarding the parameters to be evaluated to determine in vivo efficacy in humans. Clinical trials of the candidate microbicides nonoxynol-9 (N9) and cellulose sulfate revealed an increase in HIV infection, vaginal inflammation, and recruitment of HIV susceptible lymphocytes, highlighting the need to identify biomarkers that can accurately predict microbicide toxicity early in preclinical development and in human trials. We used quantitative proteomics and RT-PCR approaches in mice and rabbits to identify protein changes in vaginal fluid and tissue in response to treatment with N9 or benzalkonium chloride (BZK). We compared changes generated with N9 and BZK treatment to the changes generated in response to tenofovir gel, a candidate microbicide that holds promise as a safe and effective microbicide. Both compounds down regulated mucin 5 subtype B, and peptidoglycan recognition protein 1 in vaginal tissue; however, mucosal brush samples also showed upregulation of plasma proteins fibrinogen, plasminogen, apolipoprotein A-1, and apolipoprotein C-1, which may be a response to the erosive nature of N9 and BZK. Additional proteins down-regulated in vaginal tissue by N9 or BZK treatment include CD166 antigen, olfactomedin-4, and anterior gradient protein 2 homolog. We also observed increases in the expression of C-C chemokines CCL3, CCL5, and CCL7 in response to treatment. There was concordance in expression level changes for several of these proteins using both the mouse and rabbit models. Using a human vaginal epithelial cell line, the expression of mucin 5 subtype B and olfactomedin-4 were down-regulated in response to N9, suggesting these markers could apply to humans. These data identifies new proteins that after further validation could become part of a panel of biomarkers to effectively evaluate microbicide toxicity.


Assuntos
Citocinas/biossíntese , Genitália Feminina/efeitos dos fármacos , Fator Estimulador de Colônias de Granulócitos/biossíntese , Infecções por HIV/tratamento farmacológico , Mucina-5B/biossíntese , Administração Intravaginal , Animais , Anti-Infecciosos/efeitos adversos , Compostos de Benzalcônio/efeitos adversos , Biomarcadores/metabolismo , Celulose/efeitos adversos , Celulose/análogos & derivados , Feminino , Regulação da Expressão Gênica/efeitos dos fármacos , Genitália Feminina/metabolismo , Genitália Feminina/patologia , Infecções por HIV/metabolismo , Infecções por HIV/patologia , Humanos , Camundongos , Nonoxinol/efeitos adversos , Coelhos , Vagina/efeitos dos fármacos , Vagina/metabolismo
6.
J Proteome Res ; 13(12): 5909-17, 2014 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-25345801

RESUMO

To identify new cardiac biomarkers, a quantitative proteomic analysis has been performed on serum and heart tissue proteins from three species of nonhuman primates following isoproterenol (ISO) treatment. Three serum proteins--serum amyloid A (SAA), α-1-acid glycoprotein (A1AG), and apolipoprotein A-1 (Apo A1)--were consistently identified as changed and remained altered 72 h post dose in all three species post ISO treatment, indicating the potential of including these proteins in preclinical or clinical evaluation of drug-induced cardiac injury. Furthermore, proteomic analysis of heart tissue proteins following ISO treatment demonstrated detrimental effects on calcium signaling and energy generation in cardiac myocytes. It is worth noting that cardiac troponins were not identified in serum but were identified as altered in heart tissue lysate along with other cardiac-specific proteins. This strategy for cardiac biomarker discovery by proteomic screening of heart tissue proteins, followed by verification in serum samples using immunoassays or targeted mass spectrometry, could be applied in future biomarker studies.


Assuntos
Proteínas Musculares/sangue , Animais , Biomarcadores/sangue , Feminino , Isoproterenol , Macaca fascicularis , Macaca mulatta , Masculino , Infarto do Miocárdio/sangue , Infarto do Miocárdio/induzido quimicamente , Miocárdio/metabolismo , Miocárdio/patologia , Proteoma/metabolismo , Proteômica , Espectrometria de Massas em Tandem
7.
Protein Pept Lett ; 21(5): 419-25, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-24304386

RESUMO

Tyrosine kinase inhibitors show great promise as clinical therapies, but small molecule inhibitors that are available in the clinic and under development bind to the adenosine triphosphate binding domain of the kinase, potentially limiting efficacy and selectivity. The development of antisense peptide inhibitors is a relatively unexplored area of research, and here we investigate inhibitory peptides specific for the Janus-associated kinase (JAK) family member, tyrosine kinase 2 (TYK2). We have developed peptides that are 2-3 times more selective for TYK2 than other JAK family members, with a TYK2 IC50 of 1.2 µM. In addition, TYK2 inhibitory peptides show specificity for TYK2-mediated functions over JAK1 functions in cell-based assays. These peptides provide a new tool for the development of specific peptide inhibitors for closely related tyrosine kinases.


Assuntos
Peptídeos/química , Peptídeos/farmacologia , Inibidores de Proteínas Quinases/química , Inibidores de Proteínas Quinases/farmacologia , TYK2 Quinase/antagonistas & inibidores , Sequência de Aminoácidos , Linhagem Celular , Desenho de Fármacos , Humanos , Dados de Sequência Molecular , TYK2 Quinase/química , TYK2 Quinase/metabolismo
8.
J Proteome Res ; 12(4): 1830-7, 2013 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-23473367

RESUMO

The assessment of cardiac toxicity is a major challenge in both drug development and clinical trials, and numerous marketed pharmaceuticals have been removed from the market due to unpredicted cardiac effects. Serum troponins are widely used indicators of cardiac injury; however, they are short-lived and have not been validated in preclinical animal models. In this study, we have used filter-aided sample preparation (FASP) and tandem mass tag (TMT) labeling to investigate serum protein alterations in isoproterenol-treated African green monkeys. Our results showed that the combination of FASP and TMT labeling provided highly reproducible and efficient sample preparation, which enables us to identify and quantify serum proteins with high confidence. We focused on the proteins that exhibit long-term alteration upon isoproterenol injection and discovered nine proteins exhibiting significant changes at 48 and 72 h postdosing. We further chose three proteins, serum amyloid A (SAA), frutose biphosphate aldolase A (FBAA), and fetuin A, for validation using enzyme-linked immunosorbent assay (ELISA). The serum concentration of SAA showed a ∼ 50 fold increase, while concentration of FBAA and fetuin A exhibited a significant decrease accompanying isoproterenol-induced cardiotoxicity. This work provides valuable insights for multimarker evaluation of long-term cardiac injury.


Assuntos
Biomarcadores/sangue , Proteínas Sanguíneas/metabolismo , Coração/efeitos dos fármacos , Isoproterenol/efeitos adversos , Animais , Proteínas Sanguíneas/análise , Chlorocebus aethiops , Ensaio de Imunoadsorção Enzimática , Frutose-Bifosfato Aldolase/sangue , Miocárdio/metabolismo , Miocárdio/patologia , Reprodutibilidade dos Testes , Proteína Amiloide A Sérica/análise , Proteína Amiloide A Sérica/metabolismo , Espectrometria de Massas em Tandem , alfa-2-Glicoproteína-HS/análise , alfa-2-Glicoproteína-HS/metabolismo
9.
Org Lett ; 14(3): 732-5, 2012 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-22257322

RESUMO

Serine-Proline (SP) dipeptide motifs have been shown to form unique hydrogen-bonding patterns in protein crystal structures. Peptides were designed to mimic these patterns by forming the 6 + 10 and the 9 + 10 hydrogen-bonded rings. Factors that contribute to the formation of SP turns include controlling backbone flexibility and amino acid chirality along with creating a hydrophobic environment around the intramolecular hydrogen bonds.


Assuntos
Peptidomiméticos/química , Ligação de Hidrogênio , Modelos Moleculares , Conformação Molecular , Prolina/química , Serina/química
10.
Org Lett ; 13(21): 5878-81, 2011 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-21988552

RESUMO

Aiming to design short linear peptides featuring strong intramolecular hydrogen bonds in water, a series of tetrapeptides based on the sequence Ac-Ala-Pro-Ala-Ala-NH(2) containing all possible combinations of L- and D-amino acids was synthesized. A regiospecific combination of heterochiral residues (DDLL or its mirror image LLDD) can be used to increase turn formation and stability within short peptides in water.


Assuntos
Água/química , Amidas/química , Ligação de Hidrogênio , Estrutura Molecular
11.
Biochemistry ; 50(18): 3706-12, 2011 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-21449557

RESUMO

Villin-type headpiece domains are ∼70 residue motifs that reside at the C-terminus of a variety of actin-associated proteins. Villin headpiece (HP67) is a commonly used model system for both experimental and computational studies of protein folding. HP67 is made up of two subdomains that form a tightly packed interface. The isolated C-terminal subdomain of HP67 (HP35) is one of the smallest autonomously folding proteins known. The N-terminal subdomain requires the presence of the C-terminal subdomain to fold. In the structure of HP67, a conserved salt bridge connects N- and C-terminal subdomains. This buried salt bridge between residues E39 and K70 is unusual in a small protein domain. We used mutational analysis, monitored by CD and NMR, and functional assays to determine the role of this buried salt bridge. First, the two residues in the salt bridge were replaced with strictly hydrophobic amino acids, E39M/K70M. Second, the two residues in the salt bridge were swapped, E39K/K70E. Any change from the wild-type salt bridge residues results in unfolding of the N-terminal subdomain, even when the mutations were made in a stabilized variant of HP67. The C-terminal subdomain remains folded in all mutants and is stabilized by some of the mutations. Using actin sedimentation assays, we find that a folded N-terminal domain is essential for specific actin binding. Therefore, the buried salt bridge is required for the specific folding of the N-terminal domain which confers actin-binding activity to villin-type headpiece domains, even though the residues required for this specific interaction destabilize the C-terminal subdomain.


Assuntos
Actinas/química , Proteínas dos Microfilamentos/química , Animais , Galinhas , Dicroísmo Circular , Clonagem Molecular , Análise Mutacional de DNA , Escherichia coli/genética , Escherichia coli/metabolismo , Interações Hidrofóbicas e Hidrofílicas , Espectroscopia de Ressonância Magnética/métodos , Ligação Proteica , Conformação Proteica , Desnaturação Proteica , Dobramento de Proteína , Estrutura Terciária de Proteína , Sais/química , Termodinâmica
12.
J Am Chem Soc ; 132(13): 4508-9, 2010 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-20232912

RESUMO

Using a combination of an aromatic amino acid, a homoserine side chain, and a d-amino acid, a series of linear tetrapeptides were designed that adopt an "Hse turn" in water. The conformation was stabilized by intramolecular hydrogen bonds even in the presence of surrounding water molecules. In particular, the peptide with sequence H-Abz-Homoser-Ser-d-Gln-NH(2) showed significant through-space interactions and its free energy of folding is estimated to be on the order of -4 kcal/mol. We report the design of the tetrapeptides using a novel mimicry approach and their characterization based on NMR spectroscopy and MD simulations.


Assuntos
Peptídeos/química , Peptídeos/síntese química , Água/química , Ligação de Hidrogênio , Espectroscopia de Ressonância Magnética , Conformação Molecular , Simulação de Dinâmica Molecular , Estereoisomerismo
13.
Biochemistry ; 46(49): 14206-14, 2007 Dec 11.
Artigo em Inglês | MEDLINE | ID: mdl-18001140

RESUMO

The traditional approach to studying protein folding involves applying a perturbation, usually denaturant or mutation, and determining the effect upon the free energy of folding, DeltaG0, and the activation free energy, DeltaG(not equal). Data collected as a function of the perturbation can be used to construct rate equilibrium free-energy relationships, which report on the development of interactions in the transition state for folding. We examine the use of the ionic-strength-dependent rate equilibrium free-energy relationship in protein folding using the N-terminal domain of L9, a small alpha-beta protein, as a model system. Folding is two-state for the range of ionic strength examined, 0.045-1.52 M. The plot of DeltaG(not equal) versus DeltaG0 is linear (r2= 0.918), with a slope equal to 0.45. The relatively low value of the slope indicates that the ionic-strength-dependent interactions are modestly developed in the transition state. The slope is, however, greater than that of a plot of DeltaG(not equal) versus DeltaG0 constructed by varying pH, thus demonstrating directly that ionic-strength-dependent studies probe more than simple electrostatic interactions. Potential transition movement was probed by analysis of the denaturant, ionic strength cross-interaction parameters. The values are small but nonzero and positive, suggesting a small shift of the transition state toward the native state as the protein is destabilized, i.e., Hammond behavior. The complications that arise in the interpretation of ionic-strength-dependent rate equilibrium free-energy relationships are discussed, and it is concluded that the ionic-strength-dependent studies do not provide a reliable indicator of the role of electrostatic interactions. Complications include incomplete screening of electrostatic interactions, specific ion binding, Hofmeister effects, and the potential presence of electrostatic interactions in the denatured state ensemble.


Assuntos
Dobramento de Proteína , Proteínas Ribossômicas/química , Cinética , Modelos Moleculares , Concentração Osmolar , Fragmentos de Peptídeos/química , Desnaturação Proteica , Termodinâmica
14.
Org Lett ; 9(24): 4935-7, 2007 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-17958432

RESUMO

Isotope-edited IR of proteins has generated considerable interest. Double labeling with 13C and 18O with high levels of isotopic enrichment is required for residue-specific resolution. Current methods for the preparation of doubly labeled amino acids give modest 18O enrichment, limiting the utility of the approach. We report a simple and economical method for preparing 13C,18O-doubly labeled N-(9-fluorenylmethoxycarbonyl)amino acids with high levels of enrichment for residues that do not require acid-labile side-chain protecting groups.


Assuntos
Aminoácidos/química , Aminoácidos/síntese química , Fluorenos/química , Fluorenos/síntese química , Proteínas/química , Isótopos de Carbono , Isótopos de Oxigênio , Sensibilidade e Especificidade , Espectrofotometria Infravermelho/métodos
15.
Biochemistry ; 46(43): 12091-9, 2007 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-17924651

RESUMO

Amyloid formation has been implicated in a wide range of human diseases including Alzheimer's disease, Parkinson's disease, and type 2 diabetes. In type 2 diabetes, islet amyloid polypeptide (IAPP, also known as amylin) forms cytotoxic amyloid deposits in the pancreas, and these are believed to contribute to the pathology of the disease. The mechanism of islet amyloid formation is not understood; however, recent proposals have invoked a role for incompletely processed proIAPP. In this model, incompletely processed proIAPP containing the N-terminal pro region is excreted and binds to heparan sulfate proteoglycans (HSPGs) of the basement membrane thereby establishing a high local concentration which can act as a seed for amyloid formation. Here we report biophysical proof-of-principle experiments designed to test the viability of this model. The model predicts that interactions with HSPGs should accelerate amyloid formation by the proIAPP processing intermediate, and this is indeed what is observed. Interaction with heparan sulfate leads to the rapid formation of an intermediate state with partial helical content which then converts, on a slower time scale, to amyloid fibrils. TEM shows that fibrils formed by the proIAPP processing intermediate in the presence and in the absence of heparan sulfate have the classic features of amyloid. Fibrils formed by the proIAPP processing intermediate are competent to seed amyloid formation by mature IAPP. The seeding experiments support a second major premise of the model, namely, that fibrils formed by the processing intermediate are capable of seeding amyloid formation by the mature peptide.


Assuntos
Amiloide/biossíntese , Diabetes Mellitus Tipo 2/metabolismo , Heparitina Sulfato/metabolismo , Sequência de Aminoácidos , Amiloide/química , Amiloide/metabolismo , Dicroísmo Circular , Diabetes Mellitus Tipo 2/patologia , Humanos , Polipeptídeo Amiloide das Ilhotas Pancreáticas , Dados de Sequência Molecular , Processamento de Proteína Pós-Traducional , Espectrofotometria Ultravioleta
16.
Biochemistry ; 46(11): 3255-61, 2007 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-17311418

RESUMO

Amyloid formation has been implicated in a wide range of human diseases, and a diverse set of proteins is involved. There is considerable interest in elucidating the interactions which lead to amyloid formation and which contribute to amyloid fibril stability. Recent attention has been focused upon the potential role of aromatic-aromatic and aromatic-hydrophobic interactions in amyloid formation by short to midsized polypeptides. Here we examine whether aromatic residues are necessary for amyloid formation by islet amyloid polypeptide (IAPP). IAPP is responsible for the formation of islet amyloid in type II diabetes which is thought to play a role in the pathology of the disease. IAPP is 37 residues in length and contains three aromatic residues, Phe-15, Phe-23, and Tyr-37. Structural models of IAPP amyloid fibrils postulate that Tyr-37 is near one of the phenylalanine residues, and it is known that Tyr-37 interacts with one of the phenylalanines during fibrillization; however, it is not known if aromatic-aromatic or aromatic-hydrophobic interactions are absolutely required for amyloid formation. An F15L/F23L/Y37L triple mutant (IAPP-3XL) was prepared, and its ability to form amyloid was tested. CD, thioflavin binding assays, AFM, and TEM measurements all show that the triple leucine mutant readily forms amyloid fibrils. The substitutions do, however, decrease the rate of fibril formation and alter the tendency of fibrils to aggregate. Thus, while aromatic residues are not an absolute requirement for amyloid formation by IAPP, they do play a role in the fibril assembly process.


Assuntos
Aminoácidos Aromáticos/metabolismo , Amiloide/biossíntese , Amiloide/genética , Amiloide/metabolismo , Amiloide/ultraestrutura , Humanos , Polipeptídeo Amiloide das Ilhotas Pancreáticas , Microscopia de Força Atômica , Microscopia Eletrônica de Transmissão
17.
Biochemistry ; 46(11): 3279-85, 2007 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-17305369

RESUMO

A major difficulty in experimental studies of protein folding is the lack of nonperturbing, residue specific probes of folding. Here, we demonstrate the ability to resolve protein folding dynamics at the level of a single residue using 13C=18O isotope-edited infrared spectroscopy. A single 13C=18O isotopic label was incorporated into the backbone of the 36 residue, three-helix bundle villin headpiece subdomain (HP36). The label was placed in a solvent protected region of the second alpha-helix of the protein. The 13C=18O isotopic label shifted the carbonyl stretching frequency to 1572.1 cm-1 in the folded state, well removed from the 12C=16O band of the unlabeled protein backbone. The unique IR signature of the 13C=18O label was exploited to probe the equilibrium thermal unfolding transition using temperature-dependent FTIR spectroscopy. The folding/unfolding dynamics were monitored using temperature-jump (T-jump) IR spectroscopy. The equilibrium unfolding studies showed conformational changes suggestive of a loss of helical structure in helix 2 prior to the global unfolding of the protein. T-jump relaxation kinetics probing both the labeled site and the 12C=16O band were found to be biphasic with similar relaxation rates. The slow relaxation phase (approximately 2 x 10(5) s-1) corresponds to the global folding transition. The location of the label, a buried position in helix 2, provides an important probe of the origin of the fast relaxation phase (approximately 10(7) s-1). This phase has significant amplitude for the labeled position even though it is well protected from solvent in the folded structure. The fast phase likely represents a rapid pre-equilibrium that involves solvent penetration around the label and possible partial unfolding of helix 2 prior to the global unfolding transition. This work represents the first experimental study of ultrafast folding dynamics with residue specific resolution.


Assuntos
Proteínas de Neurofilamentos/química , Fragmentos de Peptídeos/química , Dobramento de Proteína , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Isótopos de Carbono , Cinética , Isótopos de Oxigênio , Estrutura Secundária de Proteína , Temperatura
18.
J Am Chem Soc ; 126(41): 13194-5, 2004 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-15479052

RESUMO

A major goal of protein engineering is the enhancement of protein stability. Here we demonstrate a rational method for enhancing the stability of globular proteins by targeting glycine residues which adopt conformations with Phi > 0. Replacement of such a glycine by d-alanine can lead to a significant increase in stability. The approach is tested at three sites in two model proteins. NMR and CD indicated that the substitutions do not alter the structure. Replacement of glycine-24 of the N-terminal domain of L9 (NTL9) with d-Ala results in an increase in stability of 1.3 kcal mol-1, while replacement of glycine-34 of NTL9 leads to an increase of 1.9 kcal mol-1. Replacement of glycine-331 of the UBA domain with d-Ala leads to an increase in stability of 0.6 kcal mol-1.


Assuntos
Alanina/química , Glicina/química , Proteínas Ribossômicas/química , Substituição de Aminoácidos , Modelos Moleculares , Mutagênese Sítio-Dirigida , Dobramento de Proteína , Estrutura Terciária de Proteína , Relação Estrutura-Atividade , Termodinâmica
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