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1.
J Clin Virol ; 146: 105030, 2022 01.
Artigo em Inglês | MEDLINE | ID: mdl-34839200

RESUMO

AIM AND METHODOLOGY: Several studies on hantavirus evolution have shown that genetic reassortment plays an important role in the evolution and epidemiology of this disease. To understand the genetic epidemiology of human hantaviruses, samples from rodent reservoirs were subjected to reverse-transcription nested polymerase chain reaction (RT-N-PCR) targeting the L- and S-segments of the hantavirus genome. RESULTS: Positive isolates from Gwangju, Boseong-gun (Jeollanam-do Province), and Jeju Island were confirmed as Hantaan virus using DNA sequencing. Phylogenetic analysis showed that all isolates grouped together as Hantaan virus but with each region forming a distinct cluster. In addition, these three clusters were distinct from other Hantaan isolates reported in previous studies from Korea and its neighboring countries China and Russia. CONCLUSION: This suggests Hantaan viruses exhibit a considerable degree of geographical clustering, and there may be a novel Hantaan genotype in southwestern ROK. This study helps expand our knowledge regarding the emergence of new hantavirus strains and their degree of geographical variation. IMPORTANCE: Hantaan virus, a pathogenic prototype hantavirus carried by Apodemus agrarius, is found throughout China, Russia, and Korea. Here, we examined the genetic diversity of hantaviruses to expand our knowledge regarding the emergence of new hantavirus strains and their degree of geographical variation. We found that hantaan viruses show a considerable degree of geographical clustering, which may allude to the development of a new genotype variant in the southwestern region of the ROK.


Assuntos
Orthohantavírus , Animais , Análise por Conglomerados , Genótipo , Humanos , Murinae , Filogenia , República da Coreia/epidemiologia
3.
Virol J ; 18(1): 150, 2021 07 19.
Artigo em Inglês | MEDLINE | ID: mdl-34281569

RESUMO

BACKGROUND: Mosquito-borne flaviviruses are prime pathogens and have been a major hazard to humans and animals. They comprise several arthropod-borne viruses, including dengue virus, yellow fever virus, Japanese encephalitis virus, and West Nile virus. Culex flavivirus (CxFV) is a member of the insect-specific flavivirus (ISF) group belonging to the genus Flavivirus, which is widely distributed in a variety of mosquito populations. METHODS: Viral nucleic acid was extracted from adult mosquito pools and subjected to reverse transcriptase nested polymerase chain reaction (PCR) using target-specific primers for detecting CxFV nonstructural protein 5 (NS5). The PCR-positive samples were then sequenced, and a phylogenetic tree was constructed, including reference sequences obtained from GenBank. RESULTS: 21 pools, belonging to Culex pipiens pallens (Cx. p. pallens) were found to be positive for the CxFV RNA sequence, with a minimum infection rate of 14.5/1000 mosquitoes. The phylogenetic analysis of the NS5 protein sequences indicated that the detected sequences were closely related to strains identified in China, with 95-98% sequence similarities. CONCLUSION: Our findings highlight the presence of CxFV in Cx. p. pallens mosquito species in Jeju province, Republic of Korea. This is the first study reporting the prevalence of CxFV in Culex Pipiens (Cx. pipiens) host in the Jeju province, which can create possible interaction with other flaviviruses causing human and animal diseases. Although, mosquito-borne disease causing viruses were not identified properly, more detailed surveillance and investigation of both the host and viruses are essential to understand the prevalence, evolutionary relationship and genetic characteristic with other species.


Assuntos
Culex , Flavivirus , Animais , Culex/virologia , Flavivirus/genética , Flavivirus/isolamento & purificação , Filogenia , República da Coreia
4.
Microbiol Immunol ; 62(9): 547-553, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30035807

RESUMO

Chiggers were collected from the central and southern parts of South Korea between April and November, 2009 with the aim of investigating the seasonal and geographical distribution of Or. A total of 1136 chiggers were identified. They included eight species belonging to four genera, as follows: Leptotrombidium scutellare (27.2%, n = 309), L. pallidum (54.6%, n = 621), L. orientale (6.25%, n = 71), L. palpale (1.59%, n = 18), L. zetum (2.0%, n = 23), Euschoengastia koreaensis (1.5%, n = 17), Cheladonta ikaoensis (0.08%, n = 1) and Neotrombicula japonica (1.05%, n = 12). The density of L. pallidum was high from April to May, whereas L. scutallare was not found in spring, being observed from October. Serotype-specific nested PCR targeting the 56 kDa protein gene and sequencing analysis identified that the strains of 1136 O. tsutsugamushi in the chiggers as Boryong (6.8%), Kanda (0.4%), Oishi (0.3%), Jecheon (0.1%), Youngworl (0.1%) and Wonju (0.1%). Our findings indicate that L. pallidum and L. scutellare are dominant species in Korea and have geographical and seasonal variations.


Assuntos
Biodiversidade , Orientia tsutsugamushi/classificação , Orientia tsutsugamushi/isolamento & purificação , Trombiculidae/microbiologia , Animais , Vetores Artrópodes , Clonagem Molecular , DNA Bacteriano/análise , Mapeamento Geográfico , Murinae/microbiologia , Murinae/parasitologia , Orientia tsutsugamushi/genética , Orientia tsutsugamushi/patogenicidade , Filogenia , Filogeografia , Reação em Cadeia da Polimerase , República da Coreia , Doenças dos Roedores/epidemiologia , Doenças dos Roedores/microbiologia , Tifo por Ácaros/epidemiologia , Tifo por Ácaros/microbiologia , Tifo por Ácaros/veterinária , Estações do Ano , Análise de Sequência , Especificidade da Espécie , Trombiculidae/classificação
5.
Am J Trop Med Hyg ; 98(2): 616-624, 2018 02.
Artigo em Inglês | MEDLINE | ID: mdl-29313468

RESUMO

A phylogenetic analysis of Orientia tsutsugamushi was performed to elucidate its antigenic diversity in chiggers, small mammals, and patients. Between September 2014 and December 2016, a total of 3,816 chiggers were identified within nine species of four genera in the southwest region of Korea: Leptotrombidium scutellare (49.9%; 1,907/3,816), Leptotrombidium orientale (21.1%; 804/3,816), Leptotrombidium pallidum (12.4%; 474/3,816), Euchoengastia koreaensis (7.2%; 273/3,816), Leptotrombidium palpale (6.7%; 256/3,816), Neotrombicular gardellai (1.3%; 50/3,816), Leptotrombidium zetum (0.8%; 32/3,816), Walchia fragilis (0.5%; 18/3,816), and Neotrombicular japonica (> 0.1%; 2/3,816). Twelve chiggers (11 L. scutellare and one L. palpale) tested positive for O. tsutsugamushi by polymerase chain reaction and, except for 1 chigger (KY266830), were part of the Boryong strain cluster. Of the 413 small mammals that were analyzed for O. tsutsugamushi, Apodemus agrarius was the most common rodent species (89.5%; 370/413), followed by Crocidura lasiura (6.8%; 28/413) and Myodes regulus (3.6%; 15/413). The sequence identity of an O. tsutsugamushi sample obtained from the A. agrarius sample population belonged to the Saitama strain cluster. Furthermore, a phylogenetic analysis in 125 patients revealed four clusters (Boryong cluster: 82.4% [103/125], Karp: 13.6% [17/125], Kawasaki: 3.2% [4/125], and Saitama: 0.8% [1/125]). This study clarified the phylogenetic relationship for O. tsutsugamushi in chiggers, small mammals, and patients. The Boryong strain was the most common strain in chiggers and patients. In addition, various strains were identified, except for the Boryong strain, in the southwest region of Korea. Overall, the data presented here will be helpful for the establishment of prevention strategies for scrub typhus.


Assuntos
Epidemiologia Molecular/métodos , Roedores/genética , Trombiculidae/genética , Animais , Humanos , Infestações por Ácaros/complicações , Infestações por Ácaros/epidemiologia , Orientia tsutsugamushi/genética , Orientia tsutsugamushi/patogenicidade , Reação em Cadeia da Polimerase/métodos , República da Coreia/epidemiologia , Doenças dos Roedores/epidemiologia , Doenças dos Roedores/genética
6.
J Digit Imaging ; 28(1): 62-7, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25119874

RESUMO

To save and analyze the data from a positron emission tomography/computed tomography (PET/CT) scan, it is sometimes important to use a server away from the workstation of the equipment or to install and operate mini-picture archiving and communication system (PACS). Mini-PACS was developed to save the data from a scan and measure the standard uptake value (SUV) in PACS that could be measured only in PET/CT equipment manufactured by many companies. Against this background, this study examined whether the SUV measured in PET/CT equipment was the same value in mini-PACS. This study evaluated Biograph 16 and Biograph 40 manufactured by SIEMENS and Discovery Ste 8 manufactured by GE, all of which are installed in this hospital. The SUV of the aorta of 30 patients, who had undergone an (18)F-FDG whole body PET scan in the period from February to October 2012, was measured at the height of the liver and mediastinum. In the mini-PACS program, the SUV was also measured and analyzed in an image with the same phase. According to the study results, the coefficient of the SUV of the liver in PET/CT equipment and mini-PACS was 0.99, 0.98, and 0.64 in Biograph 16, Biograph 40, and Discovery Ste 8, respectively, where the coefficient of the SUV of aorta was 0.98, 0.98, and 0.66 in Biograph 16, Biograph 40, and Discovery Ste 8, showing a positive correlation in all equipment.


Assuntos
Imagem Multimodal/métodos , Tomografia por Emissão de Pósitrons/métodos , Sistemas de Informação em Radiologia/instrumentação , Tomografia Computadorizada por Raios X/métodos , Imagem Corporal Total/métodos , Adulto , Aorta/diagnóstico por imagem , Aortografia , Fluordesoxiglucose F18 , Humanos , Fígado/diagnóstico por imagem , Compostos Radiofarmacêuticos , Reprodutibilidade dos Testes , Tomografia Computadorizada Espiral
7.
Korean J Parasitol ; 52(4): 419-23, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-25246721

RESUMO

The present study was performed to know the infection status of intestinal helminths in a most common species of field mice, Apodemus agrarius, from 2 southern regions of Korea. Total 133 and 103 mice were collected by the mouse trap in Hapcheon-gun, Gyeongsangnam-do and Gurye-gun, Jeollanam-do, respectively, from July 2005 to June 2006. The small intestine of each mouse was resected and longitudinally opened with a pair of scissors. The intestinal contents were washed with 0.85% saline until the supernatant became clear. Helminths were collected with naked eyes or under a stereomicroscope from the sediment of the intestinal content. More than 11 species of helminths (4 nematode spp., 5 trematode spp., and 2 cestode spp.) were recovered. Among these, heligmosomoid nematodes (97.5%) was the most highly and heavily infected species. As the members of trematodes, Plagiorchis muris, Brachylaima sp., Echinostoma hortense, Echinostoma cinetorchis, and unidentified echinostome larvae were found in the small intestines of 35 (14.8%), 12 (5.1%), 6 (2.5%), 1 (0.4%), and 1 (0.4%) mice respectively. Two species of tapeworms, Hymenolepis nana and Hymenolepis diminuta were also detected in 79 (33.5%) and 21 (8.9%) mice, respectively. Conclusively, heligmosomoid nematodes were the most prevalent (dominant) species among more than 11 helminth species detected, and Brachylaima sp. fluke is newly added in the list of intestinal trematodes in Korea.


Assuntos
Helmintos/classificação , Helmintos/isolamento & purificação , Enteropatias Parasitárias/veterinária , Murinae/parasitologia , Doenças dos Roedores/epidemiologia , Animais , Helmintíase/epidemiologia , Helmintíase/parasitologia , Enteropatias Parasitárias/epidemiologia , Enteropatias Parasitárias/parasitologia , Coreia (Geográfico)/epidemiologia , Prevalência , Doenças dos Roedores/parasitologia
8.
Korean J Parasitol ; 52(2): 225-9, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24850971

RESUMO

A total of 9,281 larval chigger mites were collected from small mammals captured at Hwaseong-gun, Gyeonggi-do (Province) (2,754 mites from 30 small mammals), Asan city, Chungcheongnam-do (3,358 mites from 48 mammals), and Jangseong-gun, Jeollanam-do (3,169 for 62 mammals) from April-November 2009 in the Republic of Korea (= Korea) and were identified to species. Leptotrombidium pallidum was the predominant species in Hwaseong (95.8%) and Asan (61.2%), while Leptotrombidium scutellare was the predominant species collected from Jangseong (80.1%). Overall, larval chigger mite indices decreased from April (27.3) to June (4.9), then increased in September (95.2) and to a high level in November (169.3). These data suggest that L. pallidum and L. scutellare are the primary vectors of scrub typhus throughout their range in Korea. While other species of larval chigger mites were also collected with some implications in the transmission of Orientia tsutsugamushi, they only accounted for 11.2% of all larval chigger mites collected from small mammals.


Assuntos
Larva/microbiologia , Orientia tsutsugamushi/isolamento & purificação , Tifo por Ácaros/microbiologia , Trombiculidae/classificação , Trombiculidae/microbiologia , Animais , Vetores Aracnídeos , República da Coreia , Roedores
9.
Microbiol Immunol ; 57(7): 487-95, 2013 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-23621111

RESUMO

Rickettsia monacensis, a spotted fever group rickettsia, was isolated from Ixodes nipponensis ticks collected from live-captured small mammals in South Jeolla province, Korea in 2006. Homogenates of tick tissues were inoculated into L929 and Vero cell monolayers using shell vial assays. After several passages, Giemsa staining revealed rickettsia-like organisms in the inoculated Vero cells, but not the L929 cells. Sequencing analysis revealed that the ompA-small part (25-614 bp region), ompA-large part (2849-4455 bp region), nearly full-length ompB (58-4889 bp region) and gltA (196-1236 bp region) of the isolates had similarities of 100%, 99.8%, 99.3% and 99.5%, respectively, to those of R. monacensis. Furthermore, phylogenetic analysis showed that the isolate was grouped into the cluster in the same way as R. monacensis in the trees of all genes examined. These results strongly suggest that the isolate is closely related to R. monacensis. As far as is known, this is the first report of isolation of R. monacensis from ticks in Korea.


Assuntos
Ixodes/microbiologia , Rickettsia/classificação , Rickettsia/isolamento & purificação , Animais , Proteínas de Bactérias/genética , Linhagem Celular , Análise por Conglomerados , DNA Bacteriano/química , DNA Bacteriano/genética , Humanos , Dados de Sequência Molecular , Filogenia , Rickettsia/genética , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos
10.
J Microbiol ; 46(2): 160-4, 2008 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-18545965

RESUMO

Seroepidemological investigation of antibodies to Legionella species in 500 healthy individuals from a single geographical location in Korea was conducted by indirect fluorescent antibody assay (IFA). Considering an antibody titer of > or =1:128 as positive reaction, 15.2% of total sera were positive. In males and females older than 40 years old, levels of IgM and IgG were 1.2% and 14%, respectively. The sera with antibody titers of > or =1:128 to Legionella species accounted for 85 sera, and 9 sera of these were reacted to more than one Legionella species. Reactivity to L. bozemanii, L. micdadei, L. longbeachae, L. pneumophila sg 6, and L. gormanii were 32.9%, 20%, 15%, 10.6%, and 8%, respectively. However, L. pneumophila sg 1, sg 2, and sg 3 did not react to any sera. Serological analysis revealed that the level of antibody in response to L. bozemanii was more prevalent than L. pneumophila. Our results suggest that the antibodies of non-L. pneumophila species, such as L. bozemanii, may be highly prevalent in healthy population within Korea. Although conclusions based on the findings of this study must be cautiously considered given that the population sampled were sourced from a single province, we have added to the knowledge base of serodiagnosis of infections due to non-L. pneumophila species in Korea.


Assuntos
Anticorpos Antibacterianos/sangue , Saúde , Legionella/imunologia , Legionelose/imunologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Anticorpos Antibacterianos/imunologia , Criança , Feminino , Técnica Indireta de Fluorescência para Anticorpo , Humanos , Coreia (Geográfico)/epidemiologia , Legionelose/epidemiologia , Masculino , Pessoa de Meia-Idade , Prevalência , Estudos Soroepidemiológicos
11.
Microbiol Immunol ; 51(12): 1201-7, 2007.
Artigo em Inglês | MEDLINE | ID: mdl-18094538

RESUMO

Borrelia afzelii nucleic acids were detected in the sera of febrile disease patients by a nested PCR that targeted the rrf (5S)-rrl (23S) spacer of B. burgdorferi sensu lato. The B. afzelii-specific DNA fragment was detected in 8 out of 283 sera which were proven to have immunoglobulin G or M antibodies against B. burgdorferi antigens through IFA. The results were further confirmed through restriction fragment length polymorphism and sequencing analysis of the DNA fragments. The results indicated for the first time that Lyme borreliosis is prevalent in Korea.


Assuntos
Grupo Borrelia Burgdorferi/isolamento & purificação , Doença de Lyme/diagnóstico , Doença de Lyme/microbiologia , Anticorpos Antibacterianos/sangue , Antígenos de Superfície/genética , Proteínas da Membrana Bacteriana Externa/genética , Vacinas Bacterianas/genética , Grupo Borrelia Burgdorferi/genética , DNA Bacteriano/sangue , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Espaçador Ribossômico/sangue , DNA Espaçador Ribossômico/química , DNA Espaçador Ribossômico/genética , Técnica Indireta de Fluorescência para Anticorpo , Humanos , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Coreia (Geográfico) , Lipoproteínas/genética , Dados de Sequência Molecular , Filogenia , Reação em Cadeia da Polimerase/métodos , Polimorfismo de Fragmento de Restrição , Análise de Sequência de DNA , Homologia de Sequência , Soro/microbiologia
12.
J Korean Med Sci ; 22(6): 957-62, 2007 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-18162706

RESUMO

To determine the characteristics of seroprevalence of herpes simplex virus type 2 (HSV-2) infection among Korean people, a cross-sectional study was conducted on three groups in 2004. The three groups consisted of the general public who visited public health centers, commercial sex workers (CSWs), and human immunodeficiency virus (HIV)-infected persons. Among the general public, HSV-2 seroprevalence rates for age under the 20s, in the 20s, 30s, 40s and the above 22.6%, 32.7% and 32.3%, respectively, which showed rapid increase of the rate in the 30s (p<0.0001). In case of the above of 19 yr old, women (28.0%) was higher than men (21.7%) (p<0.0001). The rate of CSWs (81.6%) was about 10 times higher than that of general women. In case of HIV-infected men (47.6%), the figure was about 2-3 times higher than that of general men. The low rate in the teens and the 20s proved that it is essential to develop sexually transmitted infections (STIs) prevention programs of education and publicity for them as a precaution measure. This study is the first major study of its kind on HSV-2 and would provide basic data for prevention of STIs including information about target groups subject to vaccination program.


Assuntos
Anticorpos Antivirais/sangue , Herpesvirus Humano 2/imunologia , Adulto , Fatores Etários , Idoso , Feminino , Infecções por HIV/complicações , Herpes Genital , Humanos , Coreia (Geográfico)/epidemiologia , Masculino , Pessoa de Meia-Idade , Estudos Soroepidemiológicos
13.
Am J Trop Med Hyg ; 76(5): 806-9, 2007 May.
Artigo em Inglês | MEDLINE | ID: mdl-17488895

RESUMO

Eschar is an important finding for the diagnosis of scrub typhus. The IFA test for possible scrub typhus was performed. The presence or absence of eschar was thoroughly examined. Among the 176 scrub typhus cases confirmed by IFA, 162 (92.0%) cases had eschar; 128 patients (79.5%) had eschars on the front of the body. Eschars were primarily detected in males within 30 cm below the umbilicus (19 patients, 35.8%). Distributions on the lower extremities and the front chest above the umbilicus were 22.6% (12 patients) and 20.8% (11 patients), respectively. A different pattern was seen in females. The most prevalent area was the front chest above the umbilicus, which accounted for 40.7% (44 patients) of all the detected eschars. Our study is the first report of a schematic diagram that shows the differences between the males and females with respect to eschar location in scrub typhus patients.


Assuntos
Vetores Aracnídeos , Mordeduras e Picadas/patologia , Ácaros , Tifo por Ácaros/patologia , Adulto , Animais , Anticorpos Antibacterianos/sangue , China , Feminino , Humanos , Masculino , Orientia tsutsugamushi/imunologia , Estudos Prospectivos , Tifo por Ácaros/diagnóstico , Fatores Sexuais
14.
Microbiol Immunol ; 51(3): 307-12, 2007.
Artigo em Inglês | MEDLINE | ID: mdl-17380050

RESUMO

This study revealed the presence of various rickettsial agents in mites from wild rodents collected in Southern Jeolla Province, Korea, by nested polymerase chain reaction (PCR) and sequence analysis of a partial citrate synthase and rickettsia outer membrane protein B genes. Rickettsial agents closely related to the Rickettsia species TwKM02, R. australis, and the Rickettsia species Cf15 were successfully identified in this study, for the first time in Korea, and R. japonica, R. akari, R. conorii, R. felis, and R. typhi were also detected, as previously described. The data presented in this paper extend knowledge on the geographic distribution of SFG rickettsiae in eastern Asia and it may necessary to consider the role of mites in rickettsial transmission.


Assuntos
Rickettsieae/genética , Trombiculidae/microbiologia , Animais , Coreia (Geográfico) , Filogenia , Reação em Cadeia da Polimerase/métodos , Rickettsieae/isolamento & purificação , Roedores/parasitologia , Análise de Sequência de DNA/métodos
15.
Am J Trop Med Hyg ; 77(6): 1120-3, 2007 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-18165533

RESUMO

We experienced a fatal case caused by Brucella abortus with multifocal necrosis confirmed by culture and polymerase chain reaction. Our case highlights that the clinician should be aware of the potential for fatality when a patient with brucellosis shows dissemination of abscess or nodules with no calcification in the liver, lung, pleura, and spine.


Assuntos
Brucella abortus/patogenicidade , Brucelose/microbiologia , Brucelose/patologia , Fígado/patologia , Pulmão/patologia , Animais , Brucella abortus/genética , Brucella abortus/isolamento & purificação , Brucelose/diagnóstico , Bovinos , Evolução Fatal , Febre , Genes Bacterianos/genética , Humanos , Coreia (Geográfico) , Fígado/diagnóstico por imagem , Dor Lombar , Pulmão/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , RNA Ribossômico 16S/genética , Tomografia Computadorizada por Raios X
16.
Korean J Parasitol ; 44(3): 243-6, 2006 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-16969063

RESUMO

In order to determine the current infection status of pond smelts, Hypomesus olidus, and other freshwater fishes with trematode metacercariae, a total of 4,861 pond smelts and 18 other freshwater fishes, collected from the Soyang and Uiam Lakes in Gangwon-do, the Unam Lake in Jeollabuk-do, the Jangseong Lake in Jeollanam-do, the Uirim-ji (lake) in Chungcheongbuk-do, and the Andong Lake in Gyeongsangbuk-do, were individually digested with 1% pepsin-HCl and examined under a dissecting microscope. In all pond smelts caught from the 6 lakes, we were unable to detect any known human infectious trematode metacercariae in Korea. However, in other freshwater fishes, such as, Squalidus japonicus coreanus (Unam Lake), and Zacco platypus (Jangseong Lake) and Opsariichthys uncirostris amurensis (Jangseong Lake), metacercariae of human-infecting trematodes, i.e., Clonorchis sinensis and Metagonimus sp. were detected, respectively.


Assuntos
Doenças dos Peixes/parasitologia , Osmeriformes/parasitologia , Infecções por Trematódeos/veterinária , Animais , Água Doce , Humanos , Coreia (Geográfico)
17.
Am J Trop Med Hyg ; 74(6): 1117-21, 2006 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-16760530

RESUMO

Several previous studies have reported a high prevalence of hepatitis C virus (HCV) infection among commercial sex workers (CSWs), but the situation is often compounded by coexisting factors, such as intravenous drug use. We conducted a seroepidemiologic study of 1,527 female CSWs in South Korea. All participants tested negative for human immunodeficiency virus and were not illicit intravenous drug users (IDUs). Twenty-one participants (1.4%) were positive for antibodies to HCV. According to the multivariate logistic regression analysis, a history of acupuncture and diabetes mellitus were associated with the seroprevalence of HCV, whereas time spent as a CSW showed only borderline significance. Sexual activity was not an independent predictor of increased risk for HCV infection in female CSWs who had never been illicit IDUs.


Assuntos
Anticorpos Anti-Hepatite C/sangue , Hepatite C/epidemiologia , Trabalho Sexual , Doenças Virais Sexualmente Transmissíveis/epidemiologia , Adulto , Demografia , Feminino , Hepatite C/virologia , Humanos , Coreia (Geográfico)/epidemiologia , Modelos Logísticos , Análise Multivariada , Fatores de Risco , Estudos Soroepidemiológicos , Comportamento Sexual , Fatores de Tempo
18.
Korean J Parasitol ; 44(1): 7-13, 2006 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-16514276

RESUMO

Heterophyid metacercarial infections in brackish water fishes, i.e., perch, shad, mullet, redlip mullet, and goby, of Jinju-man (Bay), Kyongsangnam-do, Korea, were investigated using a digestion technique. Among 45 perch (Lateolabrax japonicus), the metacercariae of Heterophyopsis continua were found in 55.6% (18.5 metacercariae per fish), Stictodora spp. in 28.9% (3.6), and Metagonimus takahashii in 6.7% (17.0). The metacercariae of H. continua were detected in 23 (65.7%) of 35 shad (Konosirus punctatus). Among 15 mullet (Mugil cephalus), the metacercariae of Pygidiopsis summa were found in 100% (105.9 metacercariae per fish), Heterophyes nocens in 40.0% (8.5), H. continua in 13.3%, and Stictodora spp. in 6.7%. Among 12 redlip mullet (Chelon haematocheilus), the metacercariae of P. summa were detected in 91.7% (1,299 metacercariae per fish), H. nocens in 16.7%, and Stictodora spp. in 16.7%. Among 35 gobies (Acanthogobius flavimanus), the metacercariae of Stictodora spp. were found in 82.9% (44.5 metacercariae per fish), and H. continua in 25.7% (2.8). The adult flukes recovered from a cat experimentally infected with metacercariae from perches were compatible with M. takahashii. The results confirm that the estuarine fish in Jinju-man (Bay) are heavily infected with heterophyid metacercariae. Furthermore, it was found for the first time that the perch acts as a second intermediate host for M. takahashii.


Assuntos
Doenças dos Peixes/epidemiologia , Doenças dos Peixes/parasitologia , Heterophyidae/isolamento & purificação , Infecções por Trematódeos/veterinária , Animais , Gatos , Peixes , Heterophyidae/anatomia & histologia , Coreia (Geográfico)/epidemiologia , Estágios do Ciclo de Vida , Prevalência , Água do Mar , Infecções por Trematódeos/epidemiologia , Infecções por Trematódeos/parasitologia
19.
Korean J Parasitol ; 43(3): 111-4, 2005 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-16192752

RESUMO

The present study investigated the prevalence rate of Cryptosporidium parvum as a cause of diarrhea. We examined 942 stools of unidentified reasons occurring in patients in whom no immunosuppression had been detected. We examined the stools for Cryptosporidium parvum via modified acid-fast staining. The clinical records of all of the positive patients were then analyzed. Nine (1%) of the stools among the 942 diarrheal patients were positive for C. parvum. The positive rate in the males was 1.1% (6/522) and the positive rate of the females was 0.7% (3/420). Age distribution revealed that the highest positive rates were in patients in their sixties, with a positive rate of 2.5% (4/158). In the clinical tests, levels of c-reactive protein, erythrocyte sedimentation rates, and neutrophil proportions were normally increased in the peripheral blood, whereas the lymphocyte proportion exhibited a tendency towards decrease. The pathological findings were compatible with an inflammatory reaction in the host.


Assuntos
Criptosporidiose/epidemiologia , Cryptosporidium , Diarreia/epidemiologia , Adulto , Idoso , Animais , Pré-Escolar , Criptosporidiose/imunologia , Cryptosporidium/isolamento & purificação , Cryptosporidium parvum/isolamento & purificação , Diarreia/imunologia , Diarreia/parasitologia , Fezes/parasitologia , Feminino , Soronegatividade para HIV , Humanos , Imunocompetência , Coreia (Geográfico)/epidemiologia , Contagem de Linfócitos , Masculino , Pessoa de Meia-Idade , Prevalência , Coloração e Rotulagem
20.
Infect Immun ; 70(8): 4692-6, 2002 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-12117985

RESUMO

Orientia tsutsugamushi shows both pro- and antiapoptotic activities in infected vertebrate cells. Apoptosis of THP-1 cells induced by beauvericin was inhibited by O. tsutsugamushi infection. Beauvericin-induced calcium redistribution was significantly reduced and retarded in cells infected with O. tsutsugamushi. Antiapoptotic activities of O. tsutsugamushi in infected cells are most probably due to inhibition of the increase in the cytosolic calcium concentration.


Assuntos
Apoptose , Cálcio/metabolismo , Depsipeptídeos , Orientia tsutsugamushi/fisiologia , Peptídeos , Antibacterianos/farmacologia , Linhagem Celular , Humanos , Líquido Intracelular , Macrófagos/citologia , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo
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