Effect of GREM 1 gene on chemoradiotherapy sensitivity of cervical squamous carcinoma cells.

OBJECTIVE: To investigate the effect of GREM1 on the sensitivity of cervical squamous carcinoma cells to radiotherapy and chemotherapy. PATIENTS AND METHODS: The resected cancer tissues and paracancerous tissues of patients with cervical carcinoma were collected. The expressions of GREM1 protein and mRNA in SiHa cell line of cervical squamous carcinoma were tested by Western blot and reverse transcription-polymerase chain reaction (qRT-PCR). The effect of GREM1 on chemotherapy sensitivity of SiHa cells was tested by MTT assay. SiHa cells were irradiated with different doses of X-ray, and the changing trend of GREM1 in cell lines was observed. RESULTS: Compared with paracancerous tissues, the expression level of GREM1 in cervical squamous carcinoma was significantly higher than that in paracancerous tissues (p<0.05). After adding different concentrations of chemotherapeutic drugs, the relative survival rate of SiHa cells overexpressing GREM1 group was significantly increased. After high-energy X-ray irradiation with different radiation doses of SiHa cells, the expression levels of GREM1mRNA and protein in SiHa cell lines showed a significant downward trend. GREM1 was highly expressed in cervical squamous carcinoma. CONCLUSIONS: Down-regulating GREM1 expression can increase the chemotherapy sensitivity of SiHa cells. GREM1 may be related to the radiotherapy sensitivity of cervical squamous carcinoma.

Clinical and experimental observations of peripheral blood leukocytes and nucleated bone marrow cells after local irradiation.

AIM: Aim of the study was to observe the impact of bone marrow damage induced by local irradiation on leukopenia. METHODS: For the human study, five cancer patients received local radiation therapy. Bone marrow aspiration was conducted to measure nucleated cell count and 99mTc-Sc sulfur colloid ECT imaging was carried out to examine bone marrow function. For the animal study, fifty New Zealand white rabbits were divided into 3 groups: non-irradiated control group (N.=10), abdomen irradiation group (irradiation area did not cover bone marrow) (N.=20), chest irradiation group (irradiation area covered bone marrow) (N.=20). Nucleated cell counts were taken after confirming onset of leukopenia. RESULTS: Bone marrow of five patients proliferated normally. ECT imaging showed no abnormality in the pattern of red bone marrow distribution. Hematopoietic function was mildly active. CONCLUSION: Suppressed myeloproliferative function does not fully account for irradiation-induced leukopenia.

Interlaboratory study of identification and quantitation of multiresidue pyrethroids in agricultural products by gas chromatography-mass spectrometry.

This paper deals with the different GC-MS analytical conditions adopted by four laboratories in an attempt to confirm the accuracy of the GC-electron-capture detection (ECD) analytical results during the international collaborative study for the establishment of the AOAC Official Method 998. 01. What is especially noted is that two laboratories have conducted comparative analysis of the respective 12 blind samples with both methods of GC-ECD and GC-MS, and the analytical results of the two methods turn out to be basically identical. This fully demonstrates that GC-MS is not only an effective confirmation tool in the analysis of the pyrethroid residues but also of sufficient sensitivity regarding the maximum residue limit of determination prescribed by FAO/WHO. Moreover, its selectivity is better than GC-ECD.