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1.
RSC Adv ; 14(31): 22086-22090, 2024 Jul 12.
Artigo em Inglês | MEDLINE | ID: mdl-39005247

RESUMO

Cold rolling has been used as a real-time surface oxidation control method to create colored strips on flexible substrates. By controlling the extrusion rate in real time, a variety of colored strips have been fabricated on Ga-based liquid metal (LM) strips. X-ray photoelectron spectroscopy (XPS) analysis shows that the surfaces of the colored strips, which were obtained through extrusion rate control of LM-Al, consist primarily of metal oxide composites, including Ga2O3, Ga2O, Al2O3, SnO2, and In2O3. The colors of the strip surfaces are directly correlated with the oxide film thickness. Additionally, these cold-rolled colored thin strips demonstrate high conductivity and have significant potential for use as conductive flexible components with indicator functions in the flexible electronics realm.

2.
Langmuir ; 40(27): 14027-14036, 2024 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-38920353

RESUMO

The accurate measurement of pH in highly alkaline environments is critical for various industrial applications but remains a complex task. This paper discusses the development of novel Fe-doped SrCoOx-based FET sensors for the detection of extreme alkaline pH levels. Through a comprehensive investigation of the effects of Fe doping on the structure, electrical properties, and sensing performance of SrCoOx, we have identified the optimal doping level that significantly enhances the sensor's performance in highly alkaline conditions. With a Fe doping level of 5 mol %, the sensitivity of the sensor improves to 0.86 lg(Ω)/pH while maintaining the response rate. Further increasing the Fe doping to 10 mol % results in a sensor that demonstrates favorable response time, a suitable pH range, and a linear correlation between lg(R) and pH. The combination of X-ray photoelectron spectroscopy and X-ray diffraction analysis provides insight into the regulation mechanisms of Fe doping on the crystal structure, electronic structure, and oxygen vacancy concentration of SrCoOx. Our findings indicate that Fe doping leads to an increase in oxygen vacancy concentration and a decrease in the energy barrier for oxygen ion migration, which contributes to the improved sensing performance of the Fe-doped SrCoOx sensors. Additionally, the study highlights the influence of oxygen vacancy concentration on the electrical properties of SrCoOx. Precise control over the concentration of oxygen vacancies is crucial for optimizing the sensitivity and response speed of SrCoOx FET sensors under extreme alkalinity conditions.

3.
Biochim Biophys Acta Gen Subj ; 1868(6): 130602, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38513927

RESUMO

BACKGROUND: High methylation of the DFNA5 gene results in the absence of GSDME, a key protein that mediates pyroptosis, while decitabine demethylates the DFNA5 gene, resulting in high expression of the GSDME protein. Cold atmospheric plasma (CAP) is a novel anti-cancer method that induces tumor cell death. METHODS: The pyroptosis induced by decitabine in combination with CAP in Ovcar5 cells was evaluated. In particular, mitochondrial membrane potential was estimated by JC-1 staining, dehydrogenase (LDH) release was assessed by ELISA, Annexin V/PI staining was detected by flow cytometry, the cell cycle changes were evaluated using PI staining followed by detection by flow cytometry, and Caspase-9 cleavage, Caspase-3 cleavage and GSDME expression were evaluated by western blot. RESULTS: Decitabine resulted in high expression of the GSDME in Ovcar5 in a concentration-dependent manner and increased tumor cell sensitivity to CAP. CAP induced mitochondrial damage and activated the Caspase-9/Caspase-3 pathway. Therefore, decitabine combined with CAP induced Ovcar5 cell pyroptosis through Caspase-3 mediated GSDME cleavage. Reactive oxygen species (ROS) generated by CAP treatment played an important role in the CAP/decitabine combination-induced production of ROS, activation of Caspase-9/Caspase-3, GSDME cleavage and pyroptosis that ROS scavenger NAC inhibited all these processes. CONCLUSIONS: CAP combined with decitabine induced Caspase-3 activation, which cleaved decitabine-upregulated GSDME and ediated pyroptosis.


Assuntos
Caspase 3 , Decitabina , Gasderminas , Gases em Plasma , Piroptose , Espécies Reativas de Oxigênio , Transdução de Sinais , Piroptose/efeitos dos fármacos , Humanos , Decitabina/farmacologia , Espécies Reativas de Oxigênio/metabolismo , Caspase 3/metabolismo , Transdução de Sinais/efeitos dos fármacos , Gases em Plasma/farmacologia , Linhagem Celular Tumoral , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Antimetabólitos Antineoplásicos/farmacologia
4.
Int J Oral Sci ; 16(1): 19, 2024 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-38418457

RESUMO

The utilization of optimal orthodontic force is crucial to prevent undesirable side effects and ensure efficient tooth movement during orthodontic treatment. However, the sensitivity of existing detection techniques is not sufficient, and the criteria for evaluating optimal force have not been yet established. Here, by employing 3D finite element analysis methodology, we found that the apical distal region (A-D region) of mesial roots is particularly sensitive to orthodontic force in rats. Tartrate-resistant acidic phosphatase (TRAP)-positive osteoclasts began accumulating in the A-D region under the force of 40 grams (g), leading to alveolar bone resorption and tooth movement. When the force reached 80 g, TRAP-positive osteoclasts started appearing on the root surface in the A-D region. Additionally, micro-computed tomography revealed a significant root resorption at 80 g. Notably, the A-D region was identified as a major contributor to whole root resorption. It was determined that 40 g is the minimum effective force for tooth movement with minimal side effects according to the analysis of tooth movement, inclination, and hyalinization. These findings suggest that the A-D region with its changes on the root surface is an important consideration and sensitive indicator when evaluating orthodontic forces for a rat model. Collectively, our investigations into this region would aid in offering valuable implications for preventing and minimizing root resorption during patients' orthodontic treatment.


Assuntos
Perda do Osso Alveolar , Reabsorção da Raiz , Humanos , Ratos , Animais , Reabsorção da Raiz/diagnóstico por imagem , Osteoclastos , Microtomografia por Raio-X , Técnicas de Movimentação Dentária , Raiz Dentária/diagnóstico por imagem , Dente Molar/diagnóstico por imagem
5.
Analyst ; 149(5): 1447-1454, 2024 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-38197456

RESUMO

Ventilator-associated pneumonia (VAP) is a prevalent disease caused by microbial infection, resulting in significant morbidity and mortality within the intensive care unit (ICU). The rapid and accurate identification of pathogenic bacteria causing VAP can assist clinicians in formulating timely treatment plans. In this study, we attempted to differentiate bacterial species in VAP by utilizing the volatile organic compounds (VOCs) released by pathogens. We cultured 6 common bacteria in VAP in vitro, including Acinetobacter baumannii, Enterobacter cloacae, Escherichia coli, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Staphylococcus aureus, which covered most cases of VAP infection in clinic. After the VOCs released by bacteria were collected in sampling bags, they were quantitatively detected by a proton transfer reaction-mass spectrometry (PTR-MS), and the characteristic ions were qualitatively analyzed through a fast gas chromatography-proton transfer reaction-mass spectrometry (FGC-PTR-MS). After conducting principal component analysis (PCA) and analysis of similarities (ANOSIM), we discovered that the VOCs released by 6 bacteria exhibited differentiation following 3 h of quantitative cultivation in vitro. Additionally, we further investigated the variations in the types and concentrations of bacterial VOCs. The results showed that by utilizing the differences in types of VOCs, 6 bacteria could be classified into 5 sets, except for A. baumannii and E. cloacae which were indistinguishable. Furthermore, we observed significant variations in the concentration ratio of acetaldehyde and methyl mercaptan released by A. baumannii and E. cloacae. In conclusion, the VOCs released by bacteria could effectively differentiate the 6 pathogens commonly associated with VAP, which was expected to assist doctors in formulating treatment plans in time and improve the survival rate of patients.


Assuntos
Pneumonia Associada à Ventilação Mecânica , Compostos Orgânicos Voláteis , Humanos , Compostos Orgânicos Voláteis/análise , Prótons , Pneumonia Associada à Ventilação Mecânica/diagnóstico , Pneumonia Associada à Ventilação Mecânica/microbiologia , Espectrometria de Massas/métodos , Bactérias
6.
Molecules ; 28(23)2023 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-38067530

RESUMO

Low-temperature plasma (LTP) is an emerging biomedical technique that has been proposed as a potential approach for cancer therapy. Meanwhile, berberine (BER), an active ingredient extracted from various medical herbs, such as Coptischinesis, has been proven antitumor effects in a broad spectrum of cancer cells. In this study, we seek to develop a novel dual cancer therapeutic method by integrating pre-administration of BER and LTP exposure and evaluating its comprehensive antitumor effect on the human non-small-cell lung cancer (NSCLC) cell lines (A549 and H1299) in vitro. Cell viability, cell cycle, cell apoptosis, and intracellular and extracellular ROS were investigated. The results showed that cotreatment of BER and LTP significantly decreased the cell viability, arrested the cell cycle in the S phase, promoted cell apoptosis, and increased intracellular and extracellular ROS. Additionally, RNA Sequencing (RNA-Seq) technology was used to explore potential mechanisms. The differentially expressed genes among different treatment groups of NSCLC cells were analyzed and were mainly enriched in the phosphatidylinositol-3-kinase (PI3K)/protein kinase B (AKT) signaling pathway. Moreover, cotreatment of BER and LTP notably depressed the total protein expression level of PI3K and AKT with immunoblotting. In conclusion, BER and LTP have a synergistic inhibitory effect on NSCLC cells via the PI3K-AKT signaling pathway, which could provide a promising strategy for supplementary therapy in the anti-NSCLC battle.


Assuntos
Berberina , Carcinoma Pulmonar de Células não Pequenas , Neoplasias Pulmonares , Humanos , Carcinoma Pulmonar de Células não Pequenas/patologia , Proteínas Proto-Oncogênicas c-akt/metabolismo , Fosfatidilinositol 3-Quinases/metabolismo , Berberina/farmacologia , Berberina/uso terapêutico , Neoplasias Pulmonares/patologia , Fosfatidilinositol 3-Quinase/metabolismo , Espécies Reativas de Oxigênio/farmacologia , Temperatura , Linhagem Celular Tumoral , Proliferação de Células , Transdução de Sinais , Apoptose
7.
J Transl Med ; 21(1): 853, 2023 11 25.
Artigo em Inglês | MEDLINE | ID: mdl-38007432

RESUMO

Pulp treatment is extremely common in endodontics, with the main purpose of eliminating clinical symptoms and preserving tooth physiological function. However, the effect of dental pulp treatment is closely related to the methods and materials used in the process of treatment. Plenty of studies about calcium silicate-based bioceramics which are widely applied in various endodontic operations have been reported because of their significant biocompatibility and bioactivity. Although most of these materials have superior physical and chemical properties, the differences between them can also have an impact on the success rate of different clinical practices. Therefore, this review is focused on the applications of several common calcium silicate-based bioceramics, including Mineral trioxide aggregate (MTA), Biodentine, Bioaggregate, iRoot BP Plus in usual endodontic treatment, such as dental pulp capping, root perforation repair, regenerative endodontic procedures (REPs), apexification, root-end filling and root canal treatment (RCT). Besides, the efficacy of these bioceramics mentioned above in human trials is also compared, which aims to provide clinical guidance for their clinical application in endodontics.


Assuntos
Materiais Restauradores do Canal Radicular , Humanos , Materiais Restauradores do Canal Radicular/farmacologia , Materiais Restauradores do Canal Radicular/uso terapêutico , Compostos de Cálcio/farmacologia , Compostos de Cálcio/uso terapêutico , Óxidos/farmacologia , Óxidos/uso terapêutico , Combinação de Medicamentos
8.
J Environ Radioact ; 270: 107303, 2023 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-37783189

RESUMO

Trichoderma harzianum has a certain resistance to Hexavalent Uranium (U(VI)), but its resistance mechanism is unknown. Based on proteomics sequencing using DIA mode, differentially expressed proteins (DEPs) of Trichoderma harzianum under U(VI) stress were identified. GO enrichment, KEGG annotation analysis and DEPs annotation were performed. The results showed that 8 DEPs, 8 DEPs and 15 DEPs were obtained in the low-dose, medium-dose and high-dose groups, respectively. The functional classification of GO demonstrated that DEPs were associated with 17 molecular functions, 5 biological processes, and 5 cellular components. Furthermore, DEPs were enriched in transport and catabolism, energy metabolism, translation, and signal transduction. These findings showed that Trichoderma harzianum was significantly changed in protein expression and signaling pathway after U(VI) exposure. Therefore, these results have provided Trichoderma harzianum with a theoretical background that can be applied to environmental cleanup.


Assuntos
Hypocreales , Monitoramento de Radiação , Trichoderma , Proteômica , Trichoderma/metabolismo
9.
Mar Pollut Bull ; 196: 115650, 2023 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-37839133

RESUMO

Marine radioactive pollution has a great impact on Marine microorganisms, but the damage mechanism by hexavalent uranium (U(VI)) exposure has been rarely known. In this study, Candida utilis (C. utilis) were exposed to U(VI) for 50, 100 and 150 mg/L, and then morphologic change and RNA-Seq in C. utilis were determined. U(VI) exposure significantly induced the changes of morphological characteristics of C. utilis. There were 39 DEGs in the 50 mg/L treated group, including 30 up-regulated genes and 9 down-regulated genes. There were 196 DEGs, 31 up-regulated and 165 down-regulated in the 100 mg/L treated group. The 150 mg/L treated group had 272 DEGs, 74 up-regulated and 198 down-regulated, compared with the control group. The results showed that the number of DEGs increased dose-dependently with U(VI) treatment. The results of this study provide a theoretical basis for the mechanism of radioactive wastewater damage to Marine microorganisms.


Assuntos
Candida , Transcriptoma , Candida/genética , Perfilação da Expressão Gênica
10.
Chemosphere ; 342: 140154, 2023 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-37714482

RESUMO

U(VI) pollution has already led to serious harm to the environment and human health with the increase of human activities. The viability of RAW264.7 cells was assessed under various U(VI) concentration stress for 24 and 48 h. The reactive oxygen species (ROS), mitochondrial membrane potential (MMP), and superoxide dismutase (SOD) activities of RAW264.7 cells under U(VI) stress were measured. The results showed that U(VI) decreased cell activity, induced intracellular ROS production, abnormal MMP, and increased SOD activity. The flow cytometry with Annexin-V/PI double labeling demonstrated that the rate of late apoptosis increased with the increase of U(VI) concentration, resulting in decreased Bcl-2 expression and increased Bax expression. The morphology of RAW264.7 cells dramatically changed after 48 h U(VI) exposure, including the evident bubble phenomenon. Besides, U(VI) also increased the proportion of LDH releases and increased GSDMD, and Ras, p38, JNK, and ERK1/2 protein expression, which indicated that the MAPK pathway was also involved. Therefore, U(VI) ultimately led to apoptosis and pyroptosis in RAW264.7 cells. This study offered convincing proof of U(VI) immunotoxicity and established the theoretical framework for further fundamental studies on U(VI) toxicity.


Assuntos
Apoptose , Piroptose , Humanos , Espécies Reativas de Oxigênio/metabolismo , Superóxido Dismutase/metabolismo
11.
Int J Mol Sci ; 24(16)2023 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-37629042

RESUMO

Atherosclerosis (AS) is an inflammatory vascular disease that constitutes a major underlying cause of cardiovascular diseases (CVD) and stroke. Infection is a contributing risk factor for AS. Epidemiological evidence has implicated individuals afflicted by periodontitis displaying an increased susceptibility to AS and CVD. This review concisely outlines several prevalent periodontal pathogens identified within atherosclerotic plaques, including Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Fusobacterium nucleatum. We review the existing epidemiological evidence elucidating the association between these pathogens and AS-related diseases, and the diverse mechanisms for which these pathogens may engage in AS, such as endothelial barrier disruption, immune system activation, facilitation of monocyte adhesion and aggregation, and promotion of foam cell formation, all of which contribute to the progression and destabilization of atherosclerotic plaques. Notably, the intricate interplay among bacteria underscores the complex impact of periodontitis on AS. In conclusion, advancing our understanding of the relationship between periodontal pathogens and AS will undoubtedly offer invaluable insights and potential therapeutic avenues for the prevention and management of AS.


Assuntos
Aterosclerose , Doenças Cardiovasculares , Placa Aterosclerótica , Humanos , Fusobacterium nucleatum , Porphyromonas gingivalis
12.
Biomolecules ; 13(7)2023 07 04.
Artigo em Inglês | MEDLINE | ID: mdl-37509109

RESUMO

This study explored the molecular mechanism of the plasma activation medium (PAM) inhibiting the migration ability of NSCLC (non-small cell lung cancer) cells. The effect of PAM incubation on the cell viability of NSCLC was detected through a cell viability experiment. Transwell cells and microfluidic chips were used to investigate the effects of PAM on the migration capacity of NSCLC cells, and the latter was used for the first time to observe the changes in the migration capacity of cancer cells treated with PAM. Moreover, the molecular mechanisms of PAM affecting the migration ability of NSCLC cells were investigated through intracellular and extracellular ROS detection, mitochondrial membrane potential, and Western blot experiments. The results showed that after long-term treatment with PAM, the high level of ROS produced by PAM reduced the level of the mitochondrial membrane potential of cells and blocked the cell division cycle in the G2/M phase. At the same time, the EMT process was reversed by inhibiting the Wnt/ß-catenin signaling pathway. These results suggested that the high ROS levels generated by the PAM treatment reversed the EMT process by inhibiting the WNT/ß-catenin pathway in NSCLC cells and thus inhibited the migration of NSCLC cells. Therefore, these results provide good theoretical support for the clinical treatment of NSCLC with PAM.


Assuntos
Carcinoma Pulmonar de Células não Pequenas , Neoplasias Pulmonares , Humanos , Carcinoma Pulmonar de Células não Pequenas/metabolismo , Neoplasias Pulmonares/tratamento farmacológico , Neoplasias Pulmonares/metabolismo , beta Catenina/metabolismo , Via de Sinalização Wnt , Espécies Reativas de Oxigênio , Temperatura , Movimento Celular , Proliferação de Células , Linhagem Celular Tumoral
13.
Biomolecules ; 13(7)2023 07 17.
Artigo em Inglês | MEDLINE | ID: mdl-37509176

RESUMO

Malocclusion is one of the three major diseases, the incidence of which could reach 56% of the imperiled oral and systemic health in the world today. Orthodontics is still the primary method to solve the problem. However, it is clear that many orthodontic complications are associated with courses of long-term therapy. Photobiomodulation (PBM) therapy could be used as a popular way to shorten the course of orthodontic treatment by nearly 26% to 40%. In this review, the efficacy in cells and animals, mechanisms, relevant cytokines and signaling, clinical trials and applications, and the future developments of PBM therapy in orthodontics were evaluated to demonstrate its validity. Simultaneously, based on orthodontic mechanisms and present findings, the mechanisms of acceleration of orthodontic tooth movement (OTM) caused by PBM therapy were explored in relation to four aspects, including blood vessels, inflammatory response, collagen and fibers, and mineralized tissues. Also, the cooperative effects and clinical translation of PBM therapy in orthodontics have been explored in a growing numbers of studies. Up to now, PBM therapy has been gaining popularity for its non-invasive nature, easy operation, and painless procedures. However, the validity and exact mechanism of PBM therapy as an adjuvant treatment in orthodontics have not been fully elucidated. Therefore, this review summarizes the efficacy of PBM therapy on the acceleration of OTM comprehensively from various aspects and was designed to provide an evidence-based platform for the research and development of light-related orthodontic tooth movement acceleration devices.


Assuntos
Terapia com Luz de Baixa Intensidade , Técnicas de Movimentação Dentária , Animais , Técnicas de Movimentação Dentária/métodos , Terapia com Luz de Baixa Intensidade/métodos , Citocinas , Adjuvantes Imunológicos , Colágeno
14.
Int J Mol Med ; 52(1)2023 07.
Artigo em Inglês | MEDLINE | ID: mdl-37264964

RESUMO

Apical periodontitis is an oral common inflammatory disease initiated by infection of pulp chamber and is characterized by destruction and resorption of the periapical bone. As a local infection, pathogens and their products in periapical tissues, as well as inflammatory cytokines produced in periapical lesions, enter the blood circulation, triggering systemic immune responses and leading to the pathogenesis of various types of systemic disease. Therefore, apical periodontitis might be associated with systemic disease rather than solely simple local oral disease. In addition, the existence of a hyperinflammatory state in certain patients with chronic inflammation­related disorder may affect the progression or prognosis of apical periodontitis. However, the association and potential mechanisms between apical periodontitis and systemic diseases remain unclear. An in­depth understanding of the association between apical periodontitis and systemic disease will be useful for both dentists and physicians to eliminate the possible risk factors and promote the healing of apical periodontitis and systemic disease. Thus, the aim of the present review is to introduce the potential relationship between apical periodontitis and systemic disease.


Assuntos
Periodontite Periapical , Humanos , Periodontite Periapical/complicações , Fatores de Risco , Citocinas , Doença Crônica
15.
Biology (Basel) ; 12(3)2023 Mar 03.
Artigo em Inglês | MEDLINE | ID: mdl-36979097

RESUMO

Radioactive elements, such as tritium, have been released into the ocean in large quantities as a result of the reactor leakage accident. In this study, an MTT assay demonstrated that the viability of HacaT cells decreased after tritiated water treatment. Bioinformatics analysis was used to analyze gene changes in the HacaT cells. The sequencing results showed 267 significantly differentially expressed genes (DEGs), and GO enrichment analysis showed that the DEGs were mainly divided into three parts. The KEGG pathway analysis showed that the up-regulated DEGs were involved in Wnt and other pathways, while the down-regulated DEGs were involved in Jak-STAT and others. A Western blot assay was used to verify the parts of the sequencing results. This study was the first to explore the mechanism of tritiated water on HacaT cells using Transcriptome analysis. The results will provide a theoretical basis for the study of tritiated water hazard mechanisms.

16.
Int J Oral Sci ; 14(1): 53, 2022 11 14.
Artigo em Inglês | MEDLINE | ID: mdl-36376275

RESUMO

Bone regeneration remains a great clinical challenge. Low intensity near-infrared (NIR) light showed strong potential to promote tissue regeneration, offering a promising strategy for bone defect regeneration. However, the effect and underlying mechanism of NIR on bone regeneration remain unclear. We demonstrated that bone regeneration in the rat skull defect model was significantly accelerated with low-intensity NIR stimulation. In vitro studies showed that NIR stimulation could promote the osteoblast differentiation in bone mesenchymal stem cells (BMSCs) and MC3T3-E1 cells, which was associated with increased ubiquitination of the core circadian clock protein Cryptochrome 1 (CRY1) in the nucleus. We found that the reduction of CRY1 induced by NIR light activated the bone morphogenetic protein (BMP) signaling pathways, promoting SMAD1/5/9 phosphorylation and increasing the expression levels of Runx2 and Osterix. NIR light treatment may act through sodium voltage-gated channel Scn4a, which may be a potential responder of NIR light to accelerate bone regeneration. Together, these findings suggest that low-intensity NIR light may promote in situ bone regeneration in a CRY1-dependent manner, providing a novel, efficient and non-invasive strategy to promote bone regeneration for clinical bone defects.


Assuntos
Regeneração Óssea , Relógios Circadianos , Criptocromos , Animais , Ratos , Proteína Morfogenética Óssea 2/metabolismo , Diferenciação Celular , Criptocromos/metabolismo , Osteoblastos/metabolismo , Osteogênese , Fatores de Transcrição/metabolismo
17.
Molecules ; 27(18)2022 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-36144670

RESUMO

Low temperature plasma (LTP) is a promising cancer therapy in clinical practice. In this study, dielectric barrier discharge plasma with helium gas was used to generate LTP. Significant increases in extracellular and intracellular reactive species were found in lung cancer cells (CALU-1 and SPC-A1) after LTP treatments. Cells viability and apoptosis assays demonstrated that LTP inhibited cells viability and induced cells death, respectively. Moreover, Western blotting revealed that the growth of CALU-1 cells was suppressed by LTP via the VEGF/VEGFR2/RAS/ERK axis for the first time. The results showed that LTP-induced ROS and RNS could inhibit the growth of lung cancer cells via VEGF/VEGFR2/RAS/ERK axis. These findings advance our understanding of the inhibitory mechanism of LTP on lung cancer and will facilitate its clinical application.


Assuntos
Neoplasias Pulmonares , Fator A de Crescimento do Endotélio Vascular , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Hélio , Humanos , Espécies Reativas de Oxigênio , Transdução de Sinais , Temperatura , Receptor 2 de Fatores de Crescimento do Endotélio Vascular , Proteínas ras/metabolismo
18.
Theor Appl Genet ; 135(1): 51-64, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34689213

RESUMO

KEY MESSAGE: qGSN5, a novel quantitative trait locus coordinating grain size and grain number in rice, was fine-mapped to an 85.60-kb region. GS3 may be a suppressor of qGSN5. Grain size and grain number are two factors that directly determine rice grain yield; however, the underlying genetic mechanisms are complicated and remain largely unclear. In this study, a chromosome segment substitution line (CSSL), CSSL28, which showed increased grain size and decreased grain number per panicle, was identified in a set of CSSLs derived from a cross between 93-11 (recipient) and Nipponbare (donor). Four substitution segments were identified in CSSL28, and the substitution segment located on chromosome 5 was responsible for the phenotypes of CSSL28. Thus, we defined this quantitative trait locus (QTL) as grain size and grain number 5 (qGSN5). Cytological and quantitative PCR analysis showed that qGSN5 regulates the development of the spikelet hull by affecting cell proliferation. Genetic analysis showed that qGSN5 is a semi-dominant locus regulating grain size and grain number. Through map-based cloning and overlapping substitution segment analysis, qGSN5 was finally delimited to an 85.60-kb region. Based on sequence and quantitative PCR analysis, Os05g47510, which encodes a P-type pentatricopeptide repeat protein, is the most likely candidate gene for qGSN5. Pyramiding analysis showed that the effect of qGSN5 was significantly lower in the presence of a functional GS3 gene, indicating that GS3 may be a suppressor of qGSN5. In addition, we found that qGSN5 could improve the grain shape of hybrid rice. Together, our results lay the foundation for cloning a novel QTL coordinating grain size and grain number in rice and provide a good genetic material for long-grain hybrid rice breeding.


Assuntos
Genes de Plantas , Oryza/genética , Locos de Características Quantitativas , Sementes/genética , Mapeamento Cromossômico , Cromossomos de Plantas , Grão Comestível/genética , Estudos de Associação Genética , Fenótipo , Sementes/anatomia & histologia
19.
J Colloid Interface Sci ; 607(Pt 2): 1730-1740, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34598030

RESUMO

A facile one-step method for synthesis of magnetic core-shell nanocomposite composed of h-Fe3O4 (hollow Fe3O4) core and stable PDA (polydopamine) shell with functional Ag NPs (silver nanoparticles) evenly distributed between them is developed. The h-Fe3O4@Ag/PDA nanocomposite showed excellent catalytic activity in the reaction for reducing azo dyes (methyl orange, methylene blue, and congo red), and the ratios of k values to the weight of h-Fe3O4@Ag/PDA were calculated to be 0.302, 0.0545, and 0.895 min-1 mg-1, respectively. Besides, the h-Fe3O4@Ag/PDA nanocomposite also exhibited good antibacterial activity in the experiment of culturing Bacillus subtilis, and the MIC (minimum inhibitory concentration) was as low as 12.5 µg/mL. Because the Ag NPs will not be leached in the solution under the protection of the PDA shell, the catalytic and antibacterial activities of h-Fe3O4@Ag/PDA nanocomposite could maintain more than 90% after five cycles. Intriguingly, this simple synthetic method can be extended to fabricate different multifunctional nanocomposites such as the spherical SiO2@Ag/PDA and rod-like Fe2O3@Ag/PDA. Overall, the facile fabrication process, the superior catalytic and antibacterial activity, and the excellent stability, endow the h-Fe3O4@Ag/PDA to be a promising nanocomposite.


Assuntos
Nanopartículas Metálicas , Nanocompostos , Antibacterianos/farmacologia , Fenômenos Magnéticos , Dióxido de Silício , Prata/farmacologia
20.
Sci Total Environ ; 786: 147443, 2021 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-33965824

RESUMO

Research evidence suggests that pesticide residues are one of the leading potential causes of the decline in pollinators, especially during vulnerable periods such as foraging in the early springtime. In China, no research quantifies pesticide residues in the nectar and pollen of honey bee colonies during this period or examines the potential risks and toxicity of pesticides to honey bees. Oilseed rape is one of the first and primary bee-attractive plants in most parts of China. Here, we investigated the pesticide residues in the oilseed rape of the years 2017 and 2018 in China. The hazard quotient (HQ) from pollen and nectar and the BeeREX risk assessment were used to evaluate the potential risks of the pesticide residues to honey bees. We detected 48 pesticides in pollen samples and 34 chemicals in nectar samples. The maximum pollen HQ (PHQ) values (contact or oral) ranged from 0.16 to 706,421, and the maximum nectar HQ (NHQ) values (contact or oral) ranged from 0.07 to 185,135. In particular, carbofuran, cyfluthrin, deltamethrin, and fenpropathrin have relatively high PHQ and NHQ values. Our results indicated that further investigation of nearly half of the tested compounds is needed because their PHQ or NHQ values are more than 50. Especially cyfluthrin and carbofuran need advanced tier assessment due to their maximum RQ (risk quotient) values exceeding the level of concern. These results provide valuable guidance for protecting bees and other pollinators in China.


Assuntos
Brassica napus , Inseticidas , Resíduos de Praguicidas , Animais , Abelhas , China , Inseticidas/análise , Resíduos de Praguicidas/análise , Resíduos de Praguicidas/toxicidade , Néctar de Plantas , Pólen/química
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