Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 3 de 3
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Cells ; 10(8)2021 07 26.
Artigo em Inglês | MEDLINE | ID: mdl-34440662

RESUMO

Neuronal culture was used to investigate neuronal function in physiological and pathological conditions. Despite its inevitability, primary neuronal culture remained a gold standard method that requires laborious preparation, intensive training, and animal resources. To circumvent the shortfalls of primary neuronal preparations and efficiently give rise to functional neurons, we combine a neural stem cell culture method with a direct cell type-conversion approach. The lucidity of this method enables the efficient preparation of functional neurons from mouse neural progenitor cells on demand. We demonstrate that induced neurons (NPC-iNs) by this method make synaptic connections, elicit neuronal activity-dependent cellular responses, and develop functional neuronal networks. This method will provide a concise platform for functional neuronal assessments. This indeed offers a perspective for using these characterized neuronal networks for investigating plasticity mechanisms, drug screening assays, and probing the molecular and biophysical basis of neurodevelopmental and neurodegenerative diseases.


Assuntos
Células-Tronco Neurais/fisiologia , Neurogênese , Animais , Técnicas de Cultura de Células , Linhagem Celular , Sinapses Elétricas/fisiologia , Potenciais Evocados , Regulação da Expressão Gênica no Desenvolvimento , Camundongos Endogâmicos C57BL , Rede Nervosa/fisiologia , Neurogênese/genética , Fenótipo , Transmissão Sináptica
3.
Development ; 146(20)2019 10 02.
Artigo em Inglês | MEDLINE | ID: mdl-31578189

RESUMO

The functions of the hippocampus are conserved between birds and mammals; however, it is not known whether similar mechanisms are responsible for its development in these two classes. In mammals, hippocampus development is known to be regulated by the hem organizer. Here, we have identified that, in birds, Wnt7b secreted from the hem is sufficient for inducing the expression of hippocampal markers. Furthermore, we have demonstrated that a microRNA, miR-19b, which is selectively excluded from the hem region, is necessary and sufficient for restricting the expression of Wnt7b to the hem. This study suggests that the role of the Wnt signal emanating from the hem is conserved between birds and mammals, and that a microRNA-based mechanism is crucial for determining the position of the hippocampus.


Assuntos
Córtex Cerebral/metabolismo , Hipocampo/metabolismo , MicroRNAs/metabolismo , Proteínas Wnt/metabolismo , Animais , Córtex Cerebral/citologia , Embrião de Galinha , Células HEK293 , Hipocampo/citologia , Humanos , Hibridização In Situ , MicroRNAs/genética , Neurogênese/genética , Neurogênese/fisiologia , Prosencéfalo/citologia , Prosencéfalo/metabolismo , Proteínas Wnt/genética
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...