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1.
Nanotechnology ; 25(21): 215602, 2014 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-24784358

RESUMO

Strain-free, vertically coupled GaAs quantum dots (QDs) with an ultra-low density below 1 × 10(7) cm(-2) are fabricated by filling of self-assembled nanoholes with a GaAs/AlGaAs/GaAs layer sequence. The sizes of the two QDs, forming a QD pair (QDP), as well as the AlGaAs tunnel-barrier between the dots are tuned independently. We present atomic force microscopy studies of the QDP formation steps. We have performed photoluminescence studies of single QDPs with varied dot size and tunnel-barrier thickness. The data indicate non-resonant tunnelling between the dots. Furthermore, we apply the quantum confined Stark effect to tune the photoluminescence energy by up to 25 meV.

2.
AORN J ; 69(5): 941-4, 1999 May.
Artigo em Inglês | MEDLINE | ID: mdl-10332550

RESUMO

Today's ORs are fast-paced. Generally, charge nurses are given little training to be effective in their role and are expected to keep the OR running smoothly. This article examines ways in which charge nurses can satisfy the needs of surgeons and staff members and offers suggestions for dealing with delays and disgruntled surgeons, fostering good working relationships, demonstrating responsibility, and maintaining a financial perspective in decision making.


Assuntos
Relações Interprofissionais , Recursos Humanos de Enfermagem Hospitalar/psicologia , Supervisão de Enfermagem/organização & administração , Enfermagem de Centro Cirúrgico/organização & administração , Atitude do Pessoal de Saúde , Comunicação , Humanos , Relações Médico-Enfermeiro , Gerenciamento do Tempo , Estados Unidos
3.
Br J Pharmacol ; 123(6): 1089-96, 1998 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-9559891

RESUMO

1. Studies were performed on isolated aortic rings without endothelium to investigate the effect of 17beta-oestradiol on cytokine-induced nitric oxide production by the inducible nitric oxide synthase (iNOS). 2. Treatment of the isolated aortic rings with interleukin-1beta (IL-1beta, 20 micro ml(-1)) led to the expression of iNOS mRNA and protein, as well as significant nitrite accumulation in the incubation media and suppression of phenylephrine (1 nM-10 microM)-evoked contraction. 3. Cycloheximide (1 microM), a protein synthesis inhibitor, prevented iNOS protein expression, nitrite accumulation and the suppression of contractility by IL-1beta on the isolated aortic rings. 17Beta-oestradiol (1 nM-10 microM) and the partial oestrogen receptor agonist 4-OH-tamoxifen (1 nM-10 microM) produced concentration-dependent inhibition of IL-1beta-induced nitrite accumulation and restored vasoconstrictor responsiveness to phenylephrine, similar to the iNOS inhibitor aminoguanidine (100 microM). 4. Semiquantitative PCR demonstrated decreased iNOS mRNA in the IL-1beta-induced and 17beta-oestradiol-treated rings. Western blot analysis of rat aorta homogenates revealed that 17beta-oestradiol treatment resulted in a reduction in IL-1beta-induced iNOS protein level. 5. Incubation with tumour necrosis factor alpha (TNF alpha, 1 ng ml(-1)) resulted in significant nitrite accumulation in the incubation media and suppression of the smooth muscle contractile response to phenylephrine, similar to IL-1beta. The effects of TNF alpha were also inhibited by co-incubation of the rings with 17beta-oestradiol and 4-OH-tamoxifen (1 microM). 6. The anti-transforming growth factor-beta1 (TGF-beta1) antibody, which inhibited TGF-beta1-induced suppression of nitrite production from IL-1beta-treated vascular rings, did not affect the inhibitory action of 17beta-oestradiol, suggesting that the effect of oestrogen on iNOS inhibition was not mediated by TGF-beta1. 7. These results show that the ovarian sex steroid, 17beta-oestradiol is a modulator of cytokine-induced iNOS activity in rat vascular smooth muscle and its mechanism of action involves decrease of iNOS mRNA and protein.


Assuntos
Aorta/efeitos dos fármacos , Estradiol/farmacologia , Interleucina-1/farmacologia , Óxido Nítrico/biossíntese , Animais , Anticorpos/imunologia , Aorta/enzimologia , Aorta/metabolismo , Técnicas In Vitro , Masculino , Contração Muscular/efeitos dos fármacos , Óxido Nítrico Sintase/genética , Óxido Nítrico Sintase Tipo II , Reação em Cadeia da Polimerase , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Ratos , Ratos Wistar , Tamoxifeno/análogos & derivados , Tamoxifeno/farmacologia , Fator de Crescimento Transformador beta/imunologia
4.
Am J Physiol ; 273(1 Pt 2): H506-9, 1997 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-9249525

RESUMO

Experiments were designed to examine the effect of 17 beta-estradiol on lipopolysaccharide (LPS)-induced excessive nitric oxide production in vivo. Ovariectomized and sham-operated female rats were injected with LPS (5 mg/kg ip), and different groups of ovariectomized, LPS-injected animals were treated with either 17 beta-estradiol, dexamethasone, or aminoguanidine. Nitric oxide generation was estimated by measuring plasma nitrite levels 12 h after LPS injection. LPS treatment of the rats resulted in a four- to fivefold increase in circulating plasma nitrite level, significantly higher in the ovariectomized animals compared with the sham-operated animals. The LPS-induced plasma nitrite increase could be prevented by dexamethasone (3 mg/kg ip) injected 1 h before LPS treatment. 17 beta-Estradiol (3 micrograms/rat sc), administered 48 h before LPS treatment, significantly attenuated the LPS-induced elevation in plasma nitrite levels. This effect was comparable to that achieved by aminoguanidine (200 microM/kg), an inhibitor of inducible nitric oxide synthase, injected 1 h after LPS treatment. The present findings suggest that estrogens may be beneficial in pathological conditions associated with excessive nitric oxide generation.


Assuntos
Estradiol/farmacologia , Lipopolissacarídeos/toxicidade , Óxido Nítrico/biossíntese , Análise de Variância , Animais , Dexametasona/farmacologia , Feminino , Guanidinas/farmacologia , Nitritos/sangue , Ovariectomia , Ratos , Ratos Wistar , Fatores de Tempo
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