Assuntos
Ácido Aspártico Endopeptidases/biossíntese , Ácido Aspártico Endopeptidases/genética , Candida albicans/enzimologia , Fungos/enzimologia , Filogenia , Sequência de Aminoácidos , Ácido Aspártico Endopeptidases/química , Sequência de Bases , Candida albicans/genética , Clonagem Molecular , DNA Complementar/metabolismo , Genes Fúngicos , Dados de Sequência Molecular , Fases de Leitura Aberta , Saccharomyces cerevisiae/enzimologia , Especificidade da EspécieRESUMO
Acid proteinases secreted by Candida tropicalis and C. parapsilosis were newly isolated. Their physico-chemical and enzymatic properties of molecular weight, pH stability, isoelectric points, specific activity, and N-terminal amino acid sequences were determined and compared with those of a C. albicans acid proteinase. The two acid proteinases secreted by C. parapsilosis were found to be new enzymes in their molecular weights. The acid proteinases from C. tropicalis and C. parapsilosis showed lower activity at neutral pH, less resistance to neutral and alkaline pH than that from C. albicans, and a half or a third of the specific activity of the C. albicans enzyme. These differences seemed to be associated with the difference of pathogenesis between Candida species. Of the 31 N-terminal amino acids, the enzymes of these three Candida species revealed 12 homologous amino acids.
Assuntos
Candida albicans/enzimologia , Candida/enzimologia , Endopeptidases/química , Sequência de Aminoácidos , Eletroforese em Gel de Poliacrilamida , Endopeptidases/metabolismo , Estabilidade Enzimática , Concentração de Íons de Hidrogênio , Ponto Isoelétrico , Dados de Sequência Molecular , Peso Molecular , Homologia de Sequência de AminoácidosRESUMO
Aureobasidins A to R were isolated from the fermentation broth of Aureobasidium pullulans R106. Aureobasidins are cyclic depsipeptide antibiotics with MW's ranging from 1.070 to 1,148. Aureobasidins showed high in vitro antifungal activity against Candida albicans.
