History of research on Aedes albopictus (Diptera: Culicidae) in Europe: approaching the world's most invasive mosquito species from a bibliometric perspective.

The Asian tiger mosquito, Aedes albopictus (Skuse), is an invasive species native to Southeast Asia. This insect, which is an important vector of arbovirus such as dengue, Zika, and chikungunya, has spread rapidly to several parts of the world over the last few decades. This study employed a bibliometric approach to explore, for the first time, Ae. albopictus research activity and output in Europe. We used the Web of Science Core Collection data source to characterize the current scientific research. A total of 903 publications from 1973 to 2022 were retrieved. We also provided a comprehensive analysis by year of publication; distribution by most productive European countries, institutions, and authors; collaboration networks; research topics; most productive journals; and most cited publications. Results showed a notable increase in the number of studies after the chikungunya virus outbreak in Northeast Italy in 2007. More than 60% of these publications across the entire European continent originated from France and Italy. Research output related to 'population and community ecology' topics was significantly high. The most common type of collaboration was national, which occurred between institutions in the same European country. By providing an overview of Ae. albopictus research in Europe, this work contributes to upcoming debates, decision-making, planning on research and development, and public health strategies on the continent and worldwide.

Evidence of interactions among apoptosis, cell proliferation, and dedifferentiation in the rudiment during whole-organ intestinal regeneration in the sea cucumber.

Sea cucumbers have an extraordinary regenerative capability. Under stressful conditions, Holothuria glaberrima can eviscerate their internal organs, including the digestive tract. From the mesentery, a rudiment grows and gives rise to a new intestine within a few weeks. In the last decades, the cellular events that occur during intestinal regeneration have been characterized, including apoptosis, cell proliferation, and muscle cell dedifferentiation. Nevertheless, their contribution to the formation and early growth of the rudiment is still unknown. Furthermore, these cellular events' relationship and potential interdependence remain a mystery. Using modulators to inhibit apoptosis and cell proliferation, we tested whether rudiment growth or other regenerative cellular events like muscle cell dedifferentiation were affected. We found that inhibition of apoptosis by zVAD and cell proliferation by aphidicolin and mitomycin did not affect the overall size of the rudiment seven days post-evisceration (7-dpe). Interestingly, animals treated with aphidicolin showed higher levels of muscle cell dedifferentiation in the distal mesentery, which could act as a compensatory mechanism. On the other hand, inhibition of apoptosis led to a decrease in cell proliferation in the rudiment and a delay in the spatiotemporal progression of muscle cell dedifferentiation throughout the rudiment-mesentery structure. Our findings suggest that neither apoptosis nor cell proliferation significantly contributes to early rudiment growth during intestinal regeneration in the sea cucumber. Nevertheless, apoptosis may play an essential role in modulating cell proliferation in the rudiment (a process known as apoptosis-induced proliferation) and the timing for the progression of muscle cell dedifferentiation. These findings provide new insights into the role and relationship of cellular events during intestinal regeneration in an emerging regeneration model.